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Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
Open accessAbstract
Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the sample is compensated by illuminating the sample consecutively from opposing directions. The resulting two images are computationally fused yielding a superior image. The effective light sheet is thinner, and the axial resolution is increased by square root 2 over single-directional SPIM. The multidirectional illumination proves essential in biological specimens such as millimeter-sized embryos. The performance of mSPIM is demonstrated by the imaging of live zebrafish embryos.
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Cited by
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Selective plane illumination microscopy techniques in developmental biology
Huisken, J., & Stainier, D. Y. R. (2009) · Development · doi:10.1242/dev.022426 · Cited by 608
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Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., et al. (2016) · eLife · doi:10.7554/elife.12068 · Cited by 359
Light-sheet microscopy: a tutorial
Olarte, O. E., Andilla, J., Gualda, E. J., & Loza-Alvarez, P. (2018) · Advances in Optics and Photonics · doi:10.1364/aop.10.000111 · Cited by 298
A versatile cortical pattern-forming circuit based on Rho, F-actin, Ect2, and RGA-3/4
Michaud, A., Leda, M., Swider, Z. T., Kim, S., He, J., Landino, J., Valley, J. R., Huisken, J., Goryachev, A. B., von Dassow, G., et al. (2022) · Journal of Cell Biology · doi:10.1083/jcb.202203017 · Cited by 59
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Metadata
- Authors
- Huisken, J., & Stainier, D. Y. R.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 2007-08-27
- Volume
- 32
- Issue
- 17
- Pages
- 2608
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2110021835
- Cited by
- 424
- References
- 0
- FWCI
- 4.95
- Type
- journal article
- Language
- en
- Open access
- green
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