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Multispectral photoacoustic imaging of fluorochromes in small animals
Abstract
Fluorochromes have become essential reporter molecules in biological research. We show that the depth-resolved distribution of fluorochromes in small animals can be imaged with 25 fmol sensitivity and 150 microm spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on self-reference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally.
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Cited by
Photoacoustic tomography: In vivo imaging from organelles to organs
Wang, L. V., & Hu, S. (2012) · Science · doi:10.1126/science.1216210 · Cited by 4,331
Going deeper than microscopy: the optical imaging frontier in biology
Ntziachristos, V. (2010) · Nature Methods · doi:10.1038/nmeth.1483 · Cited by 1,896
Self-citationPolymer-encapsulated organic nanoparticles for fluorescence and photoacoustic imaging
Li, K., & Liu, B. (2014) · Chem. Soc. Rev. · doi:10.1039/c4cs00014e · Cited by 987
Photoacoustic Imaging of Mesenchymal Stem Cells in Living Mice via Silica-Coated Gold Nanorods
Jokerst, J. V., Thangaraj, M., Kempen, P. J., Sinclair, R., & Gambhir, S. S. (2012) · ACS Nano · doi:10.1021/nn302042y · Cited by 317
Volumetric real-time multispectral optoacoustic tomography of biomarkers
Razansky, D., Buehler, A., & Ntziachristos, V. (2011) · Nature Protocols · doi:10.1038/nprot.2011.351 · Cited by 309
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Metadata
- Authors
- Razansky, D., Vinegoni, C., & Ntziachristos, V.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 2007-09-28
- Volume
- 32
- Issue
- 19
- Pages
- 2891
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2095128908
- Cited by
- 193
- References
- 0
- FWCI
- 7.77
- Type
- journal article
- Language
- en
- Open access
- closed
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