MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation
Open accessAbstract
Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein found in dental and skeletal tissues. Although information regarding the role of MEPE in bone and disorders of phosphate metabolism is emerging, the role of MEPE in dental tissues remains unclear. We performed RNA in situ hybridization and immunohistochemistry analyses to delineate the expression pattern of MEPE during embryonic and postnatal development in craniofacial mineralizing tissues. Mepe RNA expression was seen within teeth from cap through root formation in association with odontoblasts and cellular cementoblasts. More intense expression was seen in the alveolar bone within the osteoblasts and osteocytes. MEPE immunohistochemistry showed biphasic dentin staining in incisors and more intense staining in alveolar bone matrix and in forming cartilage. Analysis of Mepe null mouse molars showed overall mineralized tooth volume and density of enamel and dentin comparable with that of wild-type samples. However, Mepe -/- molars exhibited increased thickness of predentin, dentin, and enamel over controls and decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opn by RT-PCR. In vitro Mepe overexpression in odontoblasts led to significant reductions in Dspp reporter activity. These data suggest MEPE may be instrumental in craniofacial and dental matrix maturation, potentially functioning in the maintenance of non-mineralized matrix.
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Genes with HHMCs such as PLXNA2, EVC2, MEPE, OMD, and SPAG17 are known to affect craniofacial bone and tooth morphologies.
“genes with HHMCs such as PLXNA2139, EVC2140, MEPE141, OMD142, and SPAG17143 are known to affect craniofacial bone and tooth morphologies.”
From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [141]
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A catalog of single nucleotide changes distinguishing modern humans from archaic hominins
Kuhlwilm, M., & Boeckx, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-44877-x · Cited by 108 · see citing works
- Authors
- Gullard, A., Gluhak-Heinrich, J., Papagerakis, S., Sohn, P., Unterbrink, A., Chen, S., & MacDougall, M.
- Affiliations
- The University of Texas at San Antonio Health Science Center (US)
- Institute of Oral Health Research, Dental School (AG, PS, MM), University of Alabama at Birmingham, Birmingham, Alabama
- Corresponding author
- MacDougall, Mary
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 2016-02-29
- Volume
- 64
- Issue
- 4
- Pages
- 224-236
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- PMC
- PMC4817730
- OpenAlex
- W2288291608
- Cited by
- 20
- References
- 34
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 4 · 2019: 2 · 2017: 1
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Histochemistry & Cytochemistry (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (F30DE021945)
- Topics
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Keywords
- MEPE, dentin formation, odontoblasts, cementoblasts, alveolar bone, extracellular matrix maturation, enamel, predentin, dentin sialophosphoprotein, osteoblasts, osteocytes, tooth development, craniofacial development, phosphate metabolism
- MeSH terms
- Alveolar Process, Animals, Dentin, Glycoproteins, Immunohistochemistry, Mice, Inbred C57BL, Molar, Odontoblasts, Phosphoproteins, RNA, Messenger, Skull, Extracellular Matrix Proteins, Gene Deletion, In Situ Hybridization, Gene Expression Regulation, Developmental
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