Osteocytic Osteolysis in PTH-treated Wild-type and Rankl −/− Mice Examined by Transmission Electron Microscopy, Atomic Force Microscopy, and Isotope Microscopy
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To demonstrate the ultrastructure of osteocytic osteolysis and clarify whether osteocytic osteolysis occurs independently of osteoclastic activities, we examined osteocytes and their lacunae in the femora and tibiae of 11-week-old male wild-type and Rankl −/− mice after injection of human parathyroid hormone (PTH) [1-34] (80 µg/kg/dose). Serum calcium concentration rose temporarily 1 hr after PTH administration in wild-type and Rankl −/− mice, when renal arteries and veins were ligated. After 6 hr, enlargement of osteocytic lacunae was evident in the cortical bones of wild-type and Rankl −/− mice, but not so in their metaphyses. Von Kossa staining and transmission electron microscopy showed broadly demineralized bone matrix peripheral to enlarged osteocytic lacunae, which contained fragmented collagen fibrils and islets of mineralized matrices. Nano-indentation by atomic force microscopy revealed the reduced elastic modulus of the PTH-treated osteocytic perilacunar matrix, despite the microscopic verification of mineralized matrix in that region. In addition, 44 Ca deposition was detected by isotope microscopy and calcein labeling in the eroded osteocytic lacunae of wild-type and Rankl −/− mice. Taken together, our findings suggest that osteocytes can erode the bone matrix around them and deposit minerals on their lacunar walls independently of osteoclastic activity, at least in the murine cortical bone. (J Histochem Cytochem 68: –XXX, 2020)
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Three hours after PTH exposure, demineralization of the bone matrix surrounding osteocytes accompanied by compensatory mineral deposition has been observed.
“Interestingly, in addition to BMU activation, three hours after PTH exposure, demineralization of the bone matrix surrounding osteocytes accompanied by compensatory mineral deposition has been observed.82”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [82]
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- Authors
- Hongo, H., Hasegawa, T., Saito, M., Tsuboi, K., Yamamoto, T., Sasaki, M., Abe, M., Henrique Luiz de Freitas, P., Yurimoto, H., Udagawa, N., Li, M., & Amizuka, N.
- Affiliations
- Hokkaido University (JP)
- Bruker Japan K.K. (Japan) (JP)
- Haibara General Hospital (JP)
- Japan Ground Self-Defense Force (JP)
- Nagasaki University (JP)
- Department of Dentistry, Federal University of Sergipe at Lagarto, Aracaju, Brazil
- Matsumoto Dental University (JP)
- Shandong University (CN)
- Corresponding author
- Amizuka, Norio
- ORCID
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 2020-09-18
- Volume
- 68
- Issue
- 10
- Pages
- 651-668
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- PMC
- PMC7534098
- OpenAlex
- W3087310921
- Cited by
- 12
- References
- 62
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1
- FWCI
- 0.49
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Histochemistry & Cytochemistry (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- japan society for the promotion of science (18H02964, 19K10040)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Keywords
- osteocytes, osteocyte lacunae, parathyroid hormone, cortical bone, elastic modulus, calcium deposition, bone remodeling
- MeSH terms
- Animals, Injections, Intravenous, Male, Mice, Inbred ICR, Osteocytes, Osteolysis, Mice, Knockout, Microscopy, Atomic Force, Teriparatide, Microscopy, Electron, Transmission, Mice, RANK Ligand
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