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A Guide to the Perplexed on the Specificity of Antibodies
Open accessAbstract
Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.
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Cited by
Ramos-Vara, J. A., & Miller, M. A. (2014) · Veterinary Pathology · doi:10.1177/0300985813505879 · Cited by 437
Considerations for the control of background fluorescence in clinical flow cytometry
Hulspas, R., O'Gorman, M. R. G., Wood, B. L., Gratama, J. W., & Sutherland, D. R. (2009) · Cytometry Part B: Clinical Cytometry · doi:10.1002/cyto.b.20485 · Cited by 255
Antibody H3 structure prediction
Marks, C., & Deane, C. M. (2017) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2017.01.010 · Cited by 56
Kulkarni, P. S., Hurwitz, J. L., Simões, E. A. F., & Piedra, P. A. (2018) · Viral Immunology · doi:10.1089/vim.2017.0147 · Cited by 53
Angiotensin Receptor Blockers Are Not Just for Hypertension Anymore
Saavedra, J. M. (2021) · Physiology · doi:10.1152/physiol.00036.2020 · Cited by 37
Metadata
- Authors
- Saper, C. B.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 2008-09-15
- Volume
- 57
- Issue
- 1
- Pages
- 1-5
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2122743532
- Cited by
- 184
- References
- 0
- FWCI
- 4.54
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: A Guide to the Perplexed on the Specificity of Antibodies. https://app.cochat.ai/papers/10.1369/jhc.2008.952770
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- Saper, C. B. (2009). A Guide to the Perplexed on the Specificity of Antibodies. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1369/jhc.2008.952770
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
