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Single molecule imaging reveals differences in microtubule track selection between Kinesin motors

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Cai, Dawen, McEwen, Dyke P., Martens, Jeffery R., Meyhofer, Edgar, Verhey, Kristen J.·2009·PLoS Biology
DOI 10.1371/journal.pbio.1000216PMC PMC2749942
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Abstract

Cells generate diverse microtubule populations by polymerization of a common alpha/beta-tubulin building block. How microtubule associated proteins translate microtubule heterogeneity into specific cellular functions is not clear. We evaluated the ability of kinesin motors involved in vesicle transport to read microtubule heterogeneity by using single molecule imaging in live cells. We show that individual Kinesin-1 motors move preferentially on a subset of microtubules in COS cells, identified as the stable microtubules marked by post-translational modifications. In contrast, individual Kinesin-2 (KIF17) and Kinesin-3 (KIF1A) motors do not select subsets of microtubules. Surprisingly, KIF17 and KIF1A motors that overtake the plus ends of growing microtubules do not fall off but rather track with the growing tip. Selection of microtubule tracks restricts Kinesin-1 transport of VSVG vesicles to stable microtubules in COS cells whereas KIF17 transport of Kv1.5 vesicles is not restricted to specific microtubules in HL-1 myocytes. These results indicate that kinesin families can be distinguished by their ability to recognize microtubule heterogeneity. Furthermore, this property enables kinesin motors to segregate membrane trafficking events between stable and dynamic microtubule populations.

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  • Kinesin-1 moves preferentially along stable microtubules enriched in acetylation and detyrosination.

    “Given the intrinsic property of Kinesin-1 to move selectively along the subset of MTs enriched in acetylation and detyrosination (Cai et al., 2009; Dunn et al., 2008; Liao and Gundersen 1998; Kaul et al., 2014), we set out to test the application of rigor Kinesin-1 as a marker for stable MTs.”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Cai et al., 2009

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Cited by

Sorted by citations

Authors
Cai, D., McEwen, D. P., Martens, J. R., Meyhofer, E., & Verhey, K. J.
Affiliations
University of Michigan (US)
Corresponding author
Verhey, Kristen J.
ORCID
Venue
PLoS Biology
Publisher
Public Library of Science (PLoS)
Published
2009-10-13
Volume
7
Issue
10
Pages
e1000216
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.1000216
PMC
PMC2749942
OpenAlex
W2028375545
Cited by
300
References
1
Citations by year
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FWCI
6.77
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
  • National Institutes of Health (HL0270973)
  • National Heart, Lung, and Blood Institute (HL0270973)
  • National Institute on Deafness and Other Communication Disorders (T32DC000011)
  • National Institute of General Medical Sciences (GM083254, GM076476, GM070862, R01GM076476, R01GM083254, R01GM070862)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
kinesin motors, kinesin-1, KIF17, KIF1A, post-translational modification, vesicle trafficking, membrane trafficking, microtubule stability, single-molecule imaging
MeSH terms
Animals, Cell Line, Chlorocebus aethiops, Microtubules, Kinesins, COS Cells, Protein Transport, Muscle Cells, Mice, Kv1.5 Potassium Channel

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