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Cited by 146Neuroscience and Neuropharmacology ResearchNeural dynamics and brain functionMemory and Neural Mechanisms

Homeostatic Plasticity Studied Using In Vivo Hippocampal Activity-Blockade: Synaptic Scaling, Intrinsic Plasticity and Age-Dependence

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Echegoyen, Julio, Neu, Axel, Graber, Kevin D., Soltesz, Ivan·2007·PLoS ONE
DOI 10.1371/journal.pone.0000700PMC PMC1933594
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Abstract

Homeostatic plasticity is thought to be important in preventing neuronal circuits from becoming hyper- or hypoactive. However, there is little information concerning homeostatic mechanisms following in vivo manipulations of activity levels. We investigated synaptic scaling and intrinsic plasticity in CA1 pyramidal cells following 2 days of activity-blockade in vivo in adult (postnatal day 30; P30) and juvenile (P15) rats. Chronic activity-blockade in vivo was achieved using the sustained release of the sodium channel blocker tetrodotoxin (TTX) from the plastic polymer Elvax 40W implanted directly above the hippocampus, followed by electrophysiological assessment in slices in vitro. Three sets of results were in general agreement with previous studies on homeostatic responses to in vitro manipulations of activity. First, Schaffer collateral stimulation-evoked field responses were enhanced after 2 days of in vivo TTX application. Second, miniature excitatory postsynaptic current (mEPSC) amplitudes were potentiated. However, the increase in mEPSC amplitudes occurred only in juveniles, and not in adults, indicating age-dependent effects. Third, intrinsic neuronal excitability increased. In contrast, three sets of results sharply differed from previous reports on homeostatic responses to in vitro manipulations of activity. First, miniature inhibitory postsynaptic current (mIPSC) amplitudes were invariably enhanced. Second, multiplicative scaling of mEPSC and mIPSC amplitudes was absent. Third, the frequencies of adult and juvenile mEPSCs and adult mIPSCs were increased, indicating presynaptic alterations. These results provide new insights into in vivo homeostatic plasticity mechanisms with relevance to memory storage, activity-dependent development and neurological diseases.

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  • In hippocampal circuits, activity blockade can cause inhibition to change in the same direction as excitation under some conditions.

    “Further, in hippocampal circuits activity blockade has revealed that under some conditions inhibition can change in the same direction as excitation (Echegoyen et al. 2007), but whether this acts to enhance or oppose stability is not entirely clear.”

    From Homeostatic Synaptic Plasticity: Local and Global Mechanisms for Stabilizing Neuronal Function (2012) · cites this paper as Echegoyen et al. 2007

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Authors
Echegoyen, J., Neu, A., Graber, K. D., & Soltesz, I.
Affiliations
  • University of California, Irvine (US)
  • Stanford University (US)
Corresponding author
Echegoyen, Julio
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2007-08-08
Volume
2
Issue
8
Pages
e700
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0000700
PMC
PMC1933594
OpenAlex
W2093725075
Cited by
146
References
2
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 4 · 2019: 10 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 6 · 2014: 9 · 2013: 22 · 2012: 12
FWCI
3.98
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
Funders
  • Deutsche Forschungsgemeinschaft
  • National Institutes of Health (NS35915)
  • University of California, Irvine
  • National Institute of Neurological Disorders and Stroke (R29NS035915, R01NS035915, NS-35915, K08NS002167, NS02167, R37NS035915)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
homeostatic plasticity, synaptic scaling, intrinsic plasticity, tetrodotoxin, CA1 pyramidal neurons, hippocampus, age-dependent plasticity, miniature excitatory postsynaptic currents, miniature inhibitory postsynaptic currents, neuronal excitability, Schaffer collateral stimulation, field response, activity-dependent development
MeSH terms
Action Potentials, Animals, Hippocampus, Homeostasis, Nerve Net, Synaptic Transmission, Neuronal Plasticity, Tetrodotoxin, Rats, Wistar, Pyramidal Cells, Patch-Clamp Techniques, Excitatory Postsynaptic Potentials, Sodium Channel Blockers, Rats, Inhibitory Postsynaptic Potentials

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