The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Comparative Analysis of Human Gut Microbiota by Barcoded Pyrosequencing
Open accessAbstract
Humans host complex microbial communities believed to contribute to health maintenance and, when in imbalance, to the development of diseases. Determining the microbial composition in patients and healthy controls may thus provide novel therapeutic targets. For this purpose, high-throughput, cost-effective methods for microbiota characterization are needed. We have employed 454-pyrosequencing of a hyper-variable region of the 16S rRNA gene in combination with sample-specific barcode sequences which enables parallel in-depth analysis of hundreds of samples with limited sample processing. In silico modeling demonstrated that the method correctly describes microbial communities down to phylotypes below the genus level. Here we applied the technique to analyze microbial communities in throat, stomach and fecal samples. Our results demonstrate the applicability of barcoded pyrosequencing as a high-throughput method for comparative microbial ecology.
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Cites
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Cited by
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The human microbiome: at the interface of health and disease
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Jandhyala, S. M. (2015) · World Journal of Gastroenterology · doi:10.3748/wjg.v21.i29.8787 · Cited by 3,001
Metadata
- Authors
- Andersson, A. F., Lindberg, M., Jakobsson, H., Bäckhed, F., Nyrén, P., & Engstrand, L.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2008-07-30
- Volume
- 3
- Issue
- 7
- Pages
- e2836
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2066452478
- Cited by
- 1,038
- References
- 37
- FWCI
- 25.19
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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