Transfer of MicroRNAs by Embryonic Stem Cell Microvesicles
Open accessAbstract
Microvesicles are plasma membrane-derived vesicles released into the extracellular environment by a variety of cell types. Originally characterized from platelets, microvesicles are a normal constituent of human plasma, where they play an important role in maintaining hematostasis. Microvesicles have been shown to transfer proteins and RNA from cell to cell and they are also believed to play a role in intercellular communication. We characterized the RNA and protein content of embryonic stem cell microvesicles and show that they can be engineered to carry exogenously expressed mRNA and protein such as green fluorescent protein (GFP). We demonstrate that these engineered microvesicles dock and fuse with other embryonic stem cells, transferring their GFP. Additionally, we show that embryonic stem cells microvesicles contain abundant microRNA and that they can transfer a subset of microRNAs to mouse embryonic fibroblasts in vitro. Since microRNAs are short (21-24 nt), naturally occurring RNAs that regulate protein translation, our findings open up the intriguing possibility that stem cells can alter the expression of genes in neighboring cells by transferring microRNAs contained in microvesicles. Embryonic stem cell microvesicles may be useful therapeutic tools for transferring mRNA, microRNAs, protein, and siRNA to cells and may be important mediators of signaling within stem cell niches.
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microRNA's presence in exosomes has been reported in different cells and cancer types.
“microRNA's presence in exosomes have been already reported in different cells and cancer types (44–50).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [49]
The Results section reports miRNAs in embryonic stem-cell microvesicles, and the Introduction reports prior findings in peripheral-blood and ovarian-tumor microvesicles. The paper does not identify these vesicles as exosomes or establish miRNA presence across multiple cancer types. (LLM, conf=0.85)
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- Authors
- Yuan, A., Farber, E. L., Rapoport, A. L., Tejada, D., Deniskin, R., Akhmedov, N. B., & Farber, D. B.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Farber, Debora B.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-03-06
- Volume
- 4
- Issue
- 3
- Pages
- e4722
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2648987
- OpenAlex
- W2014455148
- Cited by
- 416
- References
- 3
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 7 · 2023: 7 · 2022: 10 · 2021: 11 · 2020: 13 · 2019: 24 · 2018: 25 · 2017: 23 · 2016: 41 · 2015: 46 · 2014: 39 · 2013: 37 · 2012: 52
- FWCI
- 16.63
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- Vision of Children Foundation
- Hope For Vision
- National Institutes of Health
- National Eye Institute (R21EY018739, EY018739)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- microvesicles, embryonic stem cells, microRNA transfer, extracellular vesicles, intercellular communication, microRNA delivery, mouse embryonic fibroblasts, cell fusion
- MeSH terms
- Animals, Biological Transport, Cell Communication, MicroRNAs, Green Fluorescent Proteins, Mice, Embryonic Stem Cells, Cell-Derived Microparticles
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