The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Identification of Human GnIH Homologs, RFRP-1 and RFRP-3, and the Cognate Receptor, GPR147 in the Human Hypothalamic Pituitary Axis
Open accessAbstract
The existence of a hypothalamic gonadotropin-inhibiting system has been elusive. A neuropeptide named gonadotropin-inhibitory hormone (GnIH, SIKPSAYLPLRF-NH(2)) which directly inhibits gonadotropin synthesis and release from the pituitary was recently identified in quail hypothalamus. Here we identify GnIH homologs in the human hypothalamus and characterize their distribution and biological activity. GnIH homologs were isolated from the human hypothalamus by immunoaffinity purification, and then identified as MPHSFANLPLRF-NH(2) (human RFRP-1) and VPNLPQRF-NH(2) (human RFRP-3) by mass spectrometry. Immunocytochemistry revealed GnIH-immunoreactive neuronal cell bodies in the dorsomedial region of the hypothalamus with axonal projections to GnRH neurons in the preoptic area as well as to the median eminence. RT-PCR and subsequent DNA sequencing of the PCR products identified human GnIH receptor (GPR147) mRNA expression in the hypothalamus as well as in the pituitary. In situ hybridization further identified the expression of GPR147 mRNA in luteinizing hormone producing cells (gonadotropes). Human RFRP-3 has recently been shown to be a potent inhibitor of gonadotropin secretion in cultured sheep pituitary cells by inhibiting Ca(2+) mobilization. It also directly modulates GnRH neuron firing. The identification of two forms of GnIH (RFRP-1 and RFRP-3) in the human hypothalamus which targets human GnRH neurons and gonadotropes and potently inhibit gonadotropin in sheep models provides a new paradigm for the regulation of hypothalamic-pituitary-gonadal axis in man and a novel means for manipulating reproductive functions.
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Isolation and characterization
Turksen, K. (2006) · Humana Press eBooks · Cited by 2,146
Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence
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A Novel Avian Hypothalamic Peptide Inhibiting Gonadotropin Release
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Self-citationGonadotropin-Releasing Hormone Receptors
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Self-citationNew neuropeptides containing carboxy-terminal RFamide and their receptor in mammals
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Identification and characterization of a gonadotropin-inhibitory system in the brains of mammals
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Self-citationPrimary Structure of the Ovine Hypothalamic Luteinizing Hormone-Releasing Factor (LRF)
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Self-citationSmith, J. T., Coolen, L. M., Kriegsfeld, L. J., Sari, I. P., Jaafarzadehshirazi, M. R., Maltby, M., Bateman, K., Goodman, R. L., Tilbrook, A. J., Ubuka, T., et al. (2008) · Endocrinology · doi:10.1210/en.2008-0581 · Cited by 387
Self-citationKirby, E. D., Geraghty, A. C., Ubuka, T., Bentley, G. E., & Kaufer, D. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0901176106 · Cited by 379
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Cited by
Ubuka, T., Inoue, K., Fukuda, Y., Mizuno, T., Ukena, K., Kriegsfeld, L. J., & Tsutsui, K. (2012) · Endocrinology · doi:10.1210/en.2011-1110 · Cited by 279
Self-citationKisspeptin-10 is a potent stimulator of LH and increases pulse frequency in men
George, J. T., Veldhuis, J. D., Roseweir, A. K., Newton, C. L., Faccenda, E., Millar, R. P., & Anderson, R. A. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-0089 · Cited by 186
Self-citationGlucocorticoids and reproduction: Traffic control on the road to reproduction
Whirledge, S., & Cidlowski, J. A. (2017) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2017.02.005 · Cited by 178
Knockdown of hypothalamic RFRP3 prevents chronic stress-induced infertility and embryo resorption
Geraghty, A. C., Muroy, S. E., Zhao, S., Bentley, G. E., Kriegsfeld, L. J., & Kaufer, D. (2015) · eLife · doi:10.7554/elife.04316 · Cited by 87
Self-citationIwasa, T., Matsuzaki, T., Murakami, M., Kinouchi, R., Osugi, T., Gereltsetseg, G., Yoshida, S., Irahara, M., & Tsutsui, K. (2012) · International Journal of Developmental Neuroscience · doi:10.1016/j.ijdevneu.2011.10.003 · Cited by 52
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Metadata
- Authors
- Ubuka, T., Morgan, K., Pawson, A. J., Osugi, T., Chowdhury, V. S., Minakata, H., Tsutsui, K., Millar, R. P., & Bentley, G. E.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-12-22
- Volume
- 4
- Issue
- 12
- Pages
- e8400
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2077412978
- Cited by
- 261
- References
- 43
- FWCI
- 19.46
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ubuka, T., Morgan, K., Pawson, A. J., Osugi, T., Chowdhury, V. S., Minakata, H., Tsutsui, K., Millar, R. P., & Bentley, G. E. (2009). Identification of Human GnIH Homologs, RFRP-1 and RFRP-3, and the Cognate Receptor, GPR147 in the Human Hypothalamic Pituitary Axis. PLoS ONE. https://doi.org/10.1371/journal.pone.0008400
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