Degraded Carrageenan Causing Colitis in Rats Induces TNF Secretion and ICAM-1 Upregulation in Monocytes through NF-κB Activation
Open accessAbstract
Carrageenan (CGN) is a high molecular weight sulphated polysaccharide derived from red seaweeds. In rodents, its degraded forms (dCGN) can induce intestinal inflammation associated with macrophage recruitment and activation. The aim of this study was: 1) to analyze the size-dependent effects of dCGN on colon inflammation in vivo, and 2) to correlate these effects with monocyte/macrophage proliferation, cytokine production and expression of various cell surface antigens including ICAM-1 adhesion molecule. Peripheral blood monocytes (PBM) and THP-1 monocytic cells were cultured in the presence of either 10 or 40 kDa, dCGN. The 40 kDa, but not the 10 kDa dCGN, induced colitis in in vivo. Degraded CGN inhibited THP-1 cell proliferation in vitro, arresting the cells in G1 phase. In addition, dCGN increased ICAM-1 expression in both PBM and THP-1 cells with a major effect seen after 40 kDa dCGN exposure. Also, dCGN stimulated monocyte aggregation in vitro that was prevented by incubation with anti-ICAM-1 antibody. Finally, dCGN stimulated TNF-alpha expression and secretion by both PBM and THP-1 cells. All these effects were linked to NF-kappaB activation. These data strongly suggest that the degraded forms of CGN have a pronounced effect on monocytes, characteristic of an inflammatory phenotype.
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In vitro, degraded carrageenan preparation inhibited proliferation of THP-1 cells and arrested the cells in the G1 phase.
“However, in experiments in vitro, the preparation inhibited proliferation of THP-1 cells and arrested the cells in the G1 phase [100].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [100]
Degraded carrageenan induces colitis in rats in vivo and induces inflammation.
“Later, it was confirmed that degraded carrageenan induces colitis in rats in vivo and induces inflammation.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [100]
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- Authors
- Benard, C., Cultrone, A., Michel, C., Rosales, C., Segain, J.-P., Lahaye, M., Galmiche, J.-P., Cherbut, C., & Blottière, H. M.
- Affiliations
- Microbiologie de l’alimentation au service de la santé (FR)
- PhysioPathologie des Adaptations Nutritionnelles (FR)
- Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (MX)
- Biopolymères Interactions Assemblages (FR)
- Inserm (FR)
- Nestlé (Switzerland) (CH)
- Corresponding author
- Blottière, Hervé M.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-01-13
- Volume
- 5
- Issue
- 1
- Pages
- e8666
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2800179
- OpenAlex
- W2027389535
- Cited by
- 76
- References
- 52
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 9 · 2023: 4 · 2022: 3 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 4 · 2012: 1
- FWCI
- 2.94
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, public-domain)
- PubMed Central (publishedVersion, public-domain)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Institut National de la Recherche Agronomique
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Keywords
- colitis, intestinal inflammation, ICAM-1, TNF-α, NF-κB activation, monocytes, THP-1 cells, G1 cell cycle arrest, seaweed polysaccharides, macrophage recruitment
- MeSH terms
- Animals, Base Sequence, Carrageenan, Cell Line, Colitis, Flow Cytometry, Male, Monocytes, Tumor Necrosis Factor-alpha, Blotting, Western, Up-Regulation, NF-kappa B, Rats, Wistar, DNA Primers, Intercellular Adhesion Molecule-1, Reverse Transcriptase Polymerase Chain Reaction, Rats
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