Cited by 76Seaweed-derived Bioactive CompoundsImmune Response and InflammationNF-κB Signaling Pathways

Degraded Carrageenan Causing Colitis in Rats Induces TNF Secretion and ICAM-1 Upregulation in Monocytes through NF-κB Activation

Open access
Benard, Claudine, Cultrone, Antonietta, Michel, Catherine, Rosales, Carlos, Segain, Jean-Pierre, Lahaye, Marc, Galmiche, Jean-Paul, Cherbut, Christine +1 more·2010·PLoS ONE

Abstract

Carrageenan (CGN) is a high molecular weight sulphated polysaccharide derived from red seaweeds. In rodents, its degraded forms (dCGN) can induce intestinal inflammation associated with macrophage recruitment and activation. The aim of this study was: 1) to analyze the size-dependent effects of dCGN on colon inflammation in vivo, and 2) to correlate these effects with monocyte/macrophage proliferation, cytokine production and expression of various cell surface antigens including ICAM-1 adhesion molecule. Peripheral blood monocytes (PBM) and THP-1 monocytic cells were cultured in the presence of either 10 or 40 kDa, dCGN. The 40 kDa, but not the 10 kDa dCGN, induced colitis in in vivo. Degraded CGN inhibited THP-1 cell proliferation in vitro, arresting the cells in G1 phase. In addition, dCGN increased ICAM-1 expression in both PBM and THP-1 cells with a major effect seen after 40 kDa dCGN exposure. Also, dCGN stimulated monocyte aggregation in vitro that was prevented by incubation with anti-ICAM-1 antibody. Finally, dCGN stimulated TNF-alpha expression and secretion by both PBM and THP-1 cells. All these effects were linked to NF-kappaB activation. These data strongly suggest that the degraded forms of CGN have a pronounced effect on monocytes, characteristic of an inflammatory phenotype.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0008666

Cites

52 of 52 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [2]Stanley NF 1990 Carrageenans. 79 117 In “Foods Gels”. Harris P. (Ed), Elsevier Science Publishing Co., New York

Authors
Benard, C., Cultrone, A., Michel, C., Rosales, C., Segain, J.-P., Lahaye, M., Galmiche, J.-P., Cherbut, C., & Blottière, H. M.
Affiliations
  • Microbiologie de l’alimentation au service de la santé (FR)
  • PhysioPathologie des Adaptations Nutritionnelles (FR)
  • Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (MX)
  • Biopolymères Interactions Assemblages (FR)
  • Inserm (FR)
  • Nestlé (Switzerland) (CH)
Corresponding author
Blottière, Hervé M.
Venue
PLoS ONE
Published
2010-01-13
Volume
5
Issue
1
Pages
e8666
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2027389535
Cited by
76
References
52
Citations by year
2026: 6 · 2025: 6 · 2024: 9 · 2023: 4 · 2022: 3 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 4 · 2012: 1
FWCI
2.94
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Institut National de la Recherche Agronomique
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
colitis, intestinal inflammation, ICAM-1, TNF-α, NF-κB activation, monocytes, THP-1 cells, G1 cell cycle arrest, seaweed polysaccharides, macrophage recruitment
MeSH terms
Animals, Base Sequence, Carrageenan, Cell Line, Colitis, Flow Cytometry, Male, Monocytes, Tumor Necrosis Factor-alpha, Blotting, Western, Up-Regulation, NF-kappa B, Rats, Wistar, DNA Primers, Intercellular Adhesion Molecule-1, Reverse Transcriptase Polymerase Chain Reaction, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review