The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation
Open accessAbstract
Staphylococcus aureus is a potent biofilm former on host tissue and medical implants, and biofilm growth is a critical virulence determinant for chronic infections. Recent studies suggest that many clinical isolates form polysaccharide-independent biofilms. However, a systematic screen for defective mutants has not been performed to identify factors important for biofilm formation in these strains. We created a library of 14,880 mariner transposon mutants in a S. aureus strain that generates a proteinaceous and extracellular DNA based biofilm matrix. The library was screened for biofilm defects and 31 transposon mutants conferred a reproducible phenotype. In the pool, 16 mutants overproduced extracellular proteases and the protease inhibitor alpha(2)-macroglobulin restored biofilm capacity to 13 of these mutants. The other 15 mutants in the pool displayed normal protease levels and had defects in genes involved in autolysis, osmoregulation, or uncharacterized membrane proteins. Two transposon mutants of interest in the GraRS two-component system and a putative inositol monophosphatase were confirmed in a flow cell biofilm model, genetically complemented, and further verified in a community-associated methicillin-resistant S. aureus (CA-MRSA) isolate. Collectively, our screen for biofilm defective mutants identified novel loci involved in S. aureus biofilm formation and underscored the importance of extracellular protease activity and autolysis in biofilm development.
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Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
Extracellular DNA Required for Bacterial Biofilm Formation
Whitchurch, C. B., Tolker-Nielsen, T., Ragas, P. C., & Mattick, J. S. (2002) · Science · doi:10.1126/science.295.5559.1487 · Cited by 2,203
Diep, B. A., Gill, S. R., Chang, R. F., Phan, T. H., Chen, J. H., Davidson, M. G., Lin, F., Lin, J., Carleton, H. A., Mongodin, E. F., et al. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68231-7 · Cited by 1,633
Bacterial Biofilms: An Emerging Link to Disease Pathogenesis
Parsek, M. R., & Singh, P. K. (2003) · Annual Review of Microbiology · doi:10.1146/annurev.micro.57.030502.090720 · Cited by 1,562
Sociomicrobiology: the connections between quorum sensing and biofilms
Parsek, M. R., & Greenberg, E. P. (2005) · Trends in Microbiology · doi:10.1016/j.tim.2004.11.007 · Cited by 1,174
agr-Mediated Dispersal of Staphylococcus aureus Biofilms
Boles, B. R., & Horswill, A. R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000052 · Cited by 963
Self-citationBap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation
Cucarella, C., Solano, C., Valle, J., Amorena, B., Lasa, I., & Penadés, J. R. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2888-2896.2001 · Cited by 861
Heilmann, C., Hussain, M., Peters, G., & Götz, F. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.4101774.x · Cited by 700
Horsburgh, M. J., Aish, J. L., White, I. J., Shaw, L., Lithgow, J. K., & Foster, S. J. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.19.5457-5467.2002 · Cited by 699
Cited by
Biofilm, pathogenesis and prevention—a journey to break the wall: a review
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Peptide Signaling in the Staphylococci
Thoendel, M., Kavanaugh, J. S., Flack, C. E., & Horswill, A. R. (2011) · Chemical Reviews · doi:10.1021/cr100370n · Cited by 438
Self-citationMethicillin resistance and the biofilm phenotype in Staphylococcus aureus
McCarthy, H., Rudkin, J. K., Black, N. S., Gallagher, L., O'Neill, E., & O'Gara, J. P. (2015) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2015.00001 · Cited by 426
Kaplan, J. B., Izano, E. A., Gopal, P., Karwacki, M. T., Kim, S., Bose, J. L., Bayles, K. W., & Horswill, A. R. (2012) · mBio · doi:10.1128/mbio.00198-12 · Cited by 287
Self-citationStaphylococcal biofilm disassembly
Boles, B. R., & Horswill, A. R. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.06.004 · Cited by 276
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Metadata
- Authors
- Boles, B. R., Thoendel, M., Roth, A. J., & Horswill, A. R.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-04-14
- Volume
- 5
- Issue
- 4
- Pages
- e10146
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2067215762
- Cited by
- 408
- References
- 54
- FWCI
- 7.92
- Type
- journal article
- Language
- en
- Open access
- gold
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