Candidate Glutamatergic Neurons in the Visual System of Drosophila
Open accessAbstract
The visual system of Drosophila contains approximately 60,000 neurons that are organized in parallel, retinotopically arranged columns. A large number of these neurons have been characterized in great anatomical detail. However, studies providing direct evidence for synaptic signaling and the neurotransmitter used by individual neurons are relatively sparse. Here we present a first layout of neurons in the Drosophila visual system that likely release glutamate as their major neurotransmitter. We identified 33 different types of neurons of the lamina, medulla, lobula and lobula plate. Based on the previous Golgi-staining analysis, the identified neurons are further classified into 16 major subgroups representing lamina monopolar (L), transmedullary (Tm), transmedullary Y (TmY), Y, medulla intrinsic (Mi, Mt, Pm, Dm, Mi Am), bushy T (T), translobula plate (Tlp), lobula intrinsic (Lcn, Lt, Li), lobula plate tangential (LPTCs) and lobula plate intrinsic (LPi) cell types. In addition, we found 11 cell types that were not described by the previous Golgi analysis. This classification of candidate glutamatergic neurons fosters the future neurogenetic dissection of information processing in circuits of the fly visual system.
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One wet-lab study reports a different expression from what we have predicted in some optic lobe cell types, in a study based on MARCM clones generated from drivers such as Cha-GAL4.
“We note that one wet-lab study reports a different expression from what we have predicted in some optic lobe cell types, in a study based on mosaic analysis with repressible cell marker (MARCM) clones generated from drivers such as Cha-GAL4.179”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [179]
The Results describe a wet-lab study of candidate glutamatergic neurons in optic-lobe cell types, and the Discussion compares some findings with earlier expression reports. But the Results and Materials and Methods identify the study’s approach as Flp-FRT clonal analysis using dvGlutCNSIII-Gal4, not MARCM clones generated with Cha-GAL4. The citing paper’s own predictions cannot be assessed from this source. (LLM, conf=0.87)
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Meier, M. (2011) · MPG.PuRe (Max Planck Society)
- Authors
- Raghu, S. V., & Borst, A.
- Affiliations
- Max Planck Institute of Neurobiology (DE)
- Max Planck Society (DE)
- Corresponding author
- Raghu, Shamprasad Varija
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-05-05
- Volume
- 6
- Issue
- 5
- Pages
- e19472
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3088675
- OpenAlex
- W1993569414
- Cited by
- 58
- References
- 72
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 10 · 2013: 5 · 2012: 3
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion, other-oa)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
- Keywords
- Drosophila visual system, glutamatergic neurons, neurotransmitter identification, lamina, medulla, lobula, lobula plate, neuron classification, synaptic signaling
- MeSH terms
- Animals, Drosophila, Immunohistochemistry, Microscopy, Neurons, Visual Pathways, Glutamic Acid
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