Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs
Open accessAbstract
We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.
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There were no adverse effects of feeding GE corn on small intestinal morphology or the gut microbiota.
“there were no adverse effects of feeding GE corn on small intestinal morphology or the gut microbiota.”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
Organ pathology and function were similar between animals fed GE and non-GE corn.
“Organ pathology and function were similar between animals fed GE and nonGE corn”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
There were statistically significant differences in some of the parameters measured in the two comprehensive studies.
“there were statistically significant differences in some of the parameters mea sured.”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
Results from comprehensive studies revealed the compositional and nutritional noninferiority of GE corn to its isogenic control and an absence of long-term adverse effects from GE corn consumption.
“Results from these comprehensive studies revealed the compositional and nutritional noninferiority of GE corn to its isogenic control and an absence of long-term adverse effects from GE corn consumption.”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
Antibodies specific to the GE corn protein Cry1Ab were not detected in the blood, indicating the absence of an allergic-type immune response to the protein.
“Antibodies specific to the GE corn protein (Cry1Ab) were not detected in the blood, indicating the absence of an allergic-type immune response to the protein.”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
Neither the cry1Ab gene nor the Cry1Ab protein was found in the blood, organs, or products of animals fed GE corn.
“Neither the cry1Ab gene nor the Cry1Ab protein was found in the blood, organs, or products of animals fed GE corn”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Walsh et al., 2011
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- Authors
- Walsh, M. C., Buzoianu, S. G., Gardiner, G. E., Rea, M. C., Gelencsér, E., Jánosi, A., Epstein, M. M., Ross, R. P., & Lawlor, P. G.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Waterford Institute of Technology (IE)
- Central Environmental and Food Science Research Institute (HU)
- Medical University of Vienna (AT)
- Corresponding author
- Lawlor, Peadar G.
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-11-23
- Volume
- 6
- Issue
- 11
- Pages
- e27177
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3223173
- OpenAlex
- W2005635431
- Cited by
- 82
- References
- 40
- Citations by year
- 2026: 1 · 2024: 2 · 2022: 19 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 10 · 2016: 2 · 2015: 10 · 2014: 6 · 2013: 9 · 2012: 11
- FWCI
- 10.38
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Arrow@dit (Dublin Institute of Technology) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
- Funders
- European Commission (211820, 2007–2013, FP7 2007-2013, FP7/2007)
- Teagasc
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
- Keywords
- transgenic maize, pigs, weaned piglets, immune response, growth performance, Cry1Ab toxin, IL-12, interferon-gamma, interleukin-6, IL-4, CD4+ T cells, intestinal lymphocytes, food safety
- MeSH terms
- Acclimatization, Bacillus thuringiensis Toxins, Administration, Oral, Animals, Bacillus thuringiensis, Bacterial Proteins, Body Weight, Zea mays, Diet, Endotoxins, Feeding Behavior, Hemolysin Proteins, Immunity, Leukocytes, Mycotoxins, Organ Specificity, Pesticide Residues, Swine, Time Factors, Polymerase Chain Reaction, Genes, Plant, DNA, Plant, Transgenes, Plants, Genetically Modified, Food, Genetically Modified
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