The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Polygenic Sex Determination System in Zebrafish
Open accessAbstract
BACKGROUND: Despite the popularity of zebrafish as a research model, its sex determination (SD) mechanism is still unknown. Most cytogenetic studies failed to find dimorphic sex chromosomes and no primary sex determining switch has been identified even though the assembly of zebrafish genome sequence is near to completion and a high resolution genetic map is available. Recent publications suggest that environmental factors within the natural range have minimal impact on sex ratios of zebrafish populations. The primary aim of this study is to find out more about how sex is determined in zebrafish. METHODOLOGY/PRINCIPAL FINDINGS: Using classical breeding experiments, we found that sex ratios across families were wide ranging (4.8% to 97.3% males). On the other hand, repeated single pair crossings produced broods of very similar sex ratios, indicating that parental genotypes have a role in the sex ratio of the offspring. Variation among family sex ratios was reduced after selection for breeding pairs with predominantly male or female offspring, another indication that zebrafish sex is regulated genetically. Further examinations by a PCR-based "blind assay" and array comparative genomic hybridization both failed to find universal sex-linked differences between the male and female genomes. Together with the ability to increase the sex bias of lines by selective breeding, these data suggest that zebrafish is unlikely to utilize a chromosomal sex determination (CSD) system. CONCLUSIONS/SIGNIFICANCE: Taken together, our study suggests that zebrafish sex is genetically determined with limited, secondary influences from the environment. As we have not found any sign for CSD in the species, we propose that the zebrafish has a polygenic sex determination system.
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Wild Sex in Zebrafish: Loss of the Natural Sex Determinant in Domesticated Strains
Wilson, C. A., High, S. K., McCluskey, B. M., Amores, A., Yan, Y.-L., Titus, T. A., Anderson, J. L., Batzel, P., Carvan, M. J., Schartl, M., et al. (2014) · Genetics · doi:10.1534/genetics.114.169284 · Cited by 331
Advancing biology through a deeper understanding of zebrafish ecology and evolution.
Parichy, D. M. (2015) · eLife · doi:10.7554/elife.05635 · Cited by 203
Telomerase Is Required for Zebrafish Lifespan
Henriques, C. M., Carneiro, M. C., Tenente, I. M., Jacinto, A., & Ferreira, M. G. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003214 · Cited by 133
Wiggin, T. D., Peck, J. H., & Masino, M. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0109117 · Cited by 20
Zebrafish relatives as models for functional comparative genetics and genomics
Podobnik, M., Lu, Y., Irion, U., & Currie, P. D. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-026-01007-4 · Cited by 1
Metadata
- Authors
- Liew, W. C., Bartfai, R., Lim, Z., Sreenivasan, R., Siegfried, K. R., & Orban, L.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-04-10
- Volume
- 7
- Issue
- 4
- Pages
- e34397
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2137168087
- Cited by
- 264
- References
- 63
- FWCI
- 12.76
- Type
- journal article
- Language
- en
- Open access
- gold
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