Cited by 18Fungal Infections and StudiesFungal and yeast genetics researchMicrobial Natural Products and Biosynthesis

Influence of Iron Regulation on the Metabolome of Cryptococcus neoformans

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Choi, Jung Nam, Kim, Jeongmi, Kim, Jiyoung, Jung, Won Hee, Lee, Choong Hwan·2012·PLoS ONE

Abstract

Iron is an essential nutrient for virtually all organisms and acts as a cofactor for many key enzymes of major metabolic pathways. Furthermore, iron plays a critical role in pathogen-host interactions. In this study, we analyzed metabolomic changes associated with iron availability and the iron regulatory protein Cir1 in a human fungal pathogen Cryptococcus neoformans. Our metabolite analysis revealed that Cir1 influences the glycolytic pathway, ergosterol biosynthesis and inositol metabolism, which require numerous iron-dependent enzymes and play important roles in pathogenesis and antifungal sensitivity of the fungus. Moreover, we demonstrated that increased cellular iron content and altered gene expression in the cir1 mutant contributed to metabolite changes. Our study provides a new insight into iron regulation and the role of Cir1 in metabolome of C. neoformans.

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  • An as-yet unknown Fe uptake system independent of the reductive high-affinity iron uptake system may play a role in the acidic host microenvironment in a phagolysosome.

    “These observations suggest that an as-yet unknown Fe uptake system, which is independent of the reductive high-affinity iron uptake system, may play a role in the acidic host microenvironment in a phagolysosome [79].”

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Authors
Choi, J. N., Kim, J., Kim, J., Jung, W. H., & Lee, C. H.
Affiliations
  • Konkuk University (KR)
  • Chung-Ang University (KR)
Corresponding author
Lee, Choong Hwan
Venue
PLoS ONE
Published
2012-07-23
Volume
7
Issue
7
Pages
e41654
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2034922205
Cited by
18
References
33
Citations by year
2025: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 1 · 2013: 4
FWCI
1.43
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • National Research Foundation (2010-0019306)
  • National Research Foundation of Korea (2010-0019306, 2010-0027204)
  • Ministry of Education, Science and Technology (2010-0019306)
Topics
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
Keywords
Cryptococcus neoformans, iron regulation, iron regulatory proteins, metabolome, glycolysis, ergosterol biosynthesis, inositol metabolism, antifungal susceptibility, fungal pathogenesis, iron-dependent enzymes
MeSH terms
Antifungal Agents, Azoles, Cryptococcus neoformans, Ergosterol, Fungal Proteins, Glycolysis, Humans, Inositol, Iron, Microbial Sensitivity Tests, Mutation, Mass Spectrometry, Discriminant Analysis, Least-Squares Analysis, Principal Component Analysis, Iron-Regulatory Proteins, Metabolomics, Metabolome

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