Metabolic response to iron deficiency in Saccharomyces cerevisiae
Open accessAbstract
Iron is an essential cofactor for enzymes involved in numerous cellular processes, yet little is known about the impact of iron deficiency on cellular metabolism or iron proteins. Previous studies have focused on changes in transcript and proteins levels in iron-deficient cells, yet these changes may not reflect changes in transport activity or flux through a metabolic pathway. We analyzed the metabolomes and transcriptomes of yeast grown in iron-rich and iron-poor media to determine which biosynthetic processes are altered when iron availability falls. Iron deficiency led to changes in glucose metabolism, amino acid biosynthesis, and lipid biosynthesis that were due to deficiencies in specific iron-dependent enzymes. Iron-sulfur proteins exhibited loss of iron cofactors, yet amino acid synthesis was maintained. Ergosterol and sphingolipid biosynthetic pathways had blocks at points where heme and diiron enzymes function, whereas Ole1, the essential fatty acid desaturase, was resistant to iron depletion. Iron-deficient cells exhibited depletion of most iron enzyme activities, but loss of activity during iron deficiency did not consistently disrupt metabolism. Amino acid homeostasis was robust, but iron deficiency impaired lipid synthesis, altering the properties and functions of cellular membranes.
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Erg11 function likely decreases due to the drop in heme levels when iron is scarce.
“Erg11 function is likely to decrease due to the drop in heme levels that occurs when iron is scarce”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [9]
Subsequent lanosterol accumulation may inhibit Erg1.
“the subsequent lanosterol accumulation may inhibit Erg1 [9,65].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [9]
Protein levels of both Erg1 and Erg11 decrease in response to iron depletion.
“Consistent with this, the protein levels of both Erg1 and Erg11 decrease in response to iron depletion [9].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [9]
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- Authors
- Shakoury-Elizeh, M., Protchenko, O., Berger, A., Cox, J., Gable, K., Dunn, T. M., Prinz, W. A., Bard, M., & Philpott, C. C.
- Affiliations
- National Institutes of Health (US)
- Metabolon (United States) (US)
- University of Utah (US)
- Uniformed Services University of the Health Sciences (US)
- Indiana University – Purdue University Indianapolis (US)
- Corresponding author
- Philpott, Caroline C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 285
- Issue
- 19
- Pages
- 14823-14833
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC2863190
- OpenAlex
- W2028004137
- Cited by
- 182
- References
- 48
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 9 · 2023: 14 · 2022: 12 · 2021: 14 · 2020: 13 · 2019: 11 · 2018: 11 · 2017: 10 · 2016: 11 · 2015: 10 · 2014: 8 · 2013: 13 · 2012: 13
- FWCI
- 8.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- U.S. Public Health Service
- National Institute of General Medical Sciences (R01GM062104, GM 62104)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK072437)
- National Institute of Neurological Disorders and Stroke (NS47717, R01NS047717)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- iron deficiency, Saccharomyces cerevisiae, iron metabolism, iron-sulfur proteins, glucose metabolism, amino acid biosynthesis, lipogenesis, ergosterol biosynthesis, sphingolipid biosynthesis, heme biosynthesis, metabolome, transcriptome, membrane lipid composition
- MeSH terms
- Iron Deficiencies, Saccharomyces cerevisiae, Blotting, Western, Biomarkers, Gene Expression Regulation, Fungal, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Saccharomyces cerevisiae Proteins, Immunoprecipitation, Gene Regulatory Networks, Metabolomics
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