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Additional Selection for Insecticide Resistance in Urban Malaria Vectors: DDT Resistance in Anopheles arabiensis from Bobo-Dioulasso, Burkina Faso

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Jones, Christopher M., Toé, Hyacinthe K., Sanou, Antoine, Namountougou, Moussa, Hughes, Angela, Diabaté, Abdoulaye, Dabiré, Roch, Simard, Frederic +1 more·2012·PLoS ONE
DOI 10.1371/journal.pone.0045995PMC PMC3457957
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Abstract

In the city of Bobo-Dioulasso in Burkina Faso, Anopheles arabiensis has superseded Anopheles gambiae s.s. as the major malaria vector and the larvae are found in highly polluted habitats normally considered unsuitable for Anopheles mosquitoes. Here we show that An. gambiae s.l. adults emerging from a highly polluted site in the city centre (Dioulassoba) have a high prevalence of DDT resistance (percentage mortality after exposure to diagnostic dose=65.8% in the dry season and 70.4% in the rainy season, respectively). An investigation into the mechanisms responsible found an unexpectedly high frequency of the 1014S kdr mutation (allele frequency=0.4), which is found at very low frequencies in An. arabiensis in the surrounding rural areas, and an increase in transcript levels of several detoxification genes, notably from the glutathione transferase and cytochrome P450 gene families. A number of ABC transporter genes were also expressed at elevated levels in the DDT resistant An. arabiensis. Unplanned urbanisation provides numerous breeding grounds for mosquitoes. The finding that Anopheles mosquitoes adapted to these urban breeding sites have a high prevalence of insecticide resistance has important implications for our understanding of the selective forces responsible for the rapid spread of insecticide resistant populations of malaria vectors in Africa.

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  • These mutations have also been detected with high frequencies in An. gambiae (s.l.) in many regions of West Africa, with ecological similarities in Burkina Faso and Benin.

    “These mutations have also been detected with high frequencies in An. gambiae (s.l.) in many regions of West Africa [28, 29] with ecological similarities in Burkina Faso and Benin (cotton, rice and urban/sub-urban vegetable growing areas) [30, 31].”

    From Mapping insecticide resistance in Anopheles gambiae (s.l.) from Côte d'Ivoire (2018) · cites this paper as [30]

    The Introduction says the resistance alleles are widely distributed in An. gambiae s.l., and the Results report high 1014S frequency in An. arabiensis at Dioulassoba, Burkina Faso. The Study Site and Mosquito Collections section describes urban vegetable cropping there, but the paper does not establish comparable cotton, rice, and vegetable-growing ecologies in both Burkina Faso and Benin. Page numbers are unavailable in our copy. (LLM, conf=0.84)

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Authors
Jones, C. M., Toé, H. K., Sanou, A., Namountougou, M., Hughes, A., Diabaté, A., Dabiré, R., Simard, F., & Ranson, H.
Affiliations
  • Liverpool School of Tropical Medicine (GB)
  • Centre National de Recherche et de Formation sur le Paludisme (BF)
  • Centre Muraz (BF)
Corresponding author
Jones, Christopher M.
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2012-09-25
Volume
7
Issue
9
Pages
e45995
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0045995
PMC
PMC3457957
OpenAlex
W1992417356
Cited by
97
References
4
Citations by year
2026: 1 · 2025: 1 · 2024: 8 · 2023: 8 · 2022: 6 · 2021: 8 · 2020: 2 · 2019: 13 · 2018: 8 · 2017: 6 · 2016: 6 · 2015: 7 · 2014: 15 · 2013: 8
FWCI
9.27
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LSTM Online Archive (Liverpool School of Tropical Medicine) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
UNICEF
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Social Sciences › Sociology and Political Science › Dengue and Mosquito Control Research
Keywords
DDT resistance, Anopheles arabiensis, urban malaria vectors, kdr mutation, detoxification genes, glutathione S-transferase, cytochrome P450, ABC transporters, insecticide resistance selection, urbanization, mosquito breeding sites, Anopheles gambiae s.l., Bobo-Dioulasso
MeSH terms
Animals, Anopheles, Biological Assay, Burkina Faso, DDT, Female, Gene Frequency, Genotype, Glutathione Transferase, Humans, Insecticide Resistance, Insecticides, Models, Genetic, Culicidae, Mutation, Species Specificity, Transcription, Genetic, Urbanization, Oligonucleotide Array Sequence Analysis

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