Do pollutants affect insecticide-driven gene selection in mosquitoes? Experimental evidence from transcriptomics
Abstract
The control of mosquitoes transmitting infectious diseases relies mainly on the use of chemical insecticides. However, the emergence of insecticide resistance threatens mosquito control programs. Until now, most research efforts have been focused on elucidating resistance mechanisms caused by insecticide treatments. Less attention has been paid to the impact of the mosquito chemical environment on insecticide-driven selection mechanisms. Here the mosquito Aedes aegypti was used as a model species to conduct laboratory experiments combining the exposure of mosquito larvae to a sub-lethal concentration of xenobiotics and their selection with the insecticide permethrin. After 10 generations, bioassays and a transcriptome profiling with a 15 k microarray were performed comparatively on all strains. The three selected strains showed a small but significant increase of permethrin resistance compared to the susceptible parental strain. Microarray analysis revealed that the transcription of many genes was altered by insecticide selection. Exposing larvae to sub-lethal concentrations of the pollutant fluoranthene or the insecticide permethrin prior to selection at each generation affected the selection of several genes, including those involved in detoxification, transport and cell metabolism. Genes potentially involved in permethrin resistance and cross-responses between xenobiotics and insecticide were identified. The present study investigated for the first time the impact of the presence of pollutants in mosquito environment on insecticide-driven selection mechanisms. Our results revealed that mosquitoes exposed to xenobiotics show a different adaptive response to insecticide selection pressure. This suggests that insect chemical environment can shape the long-term selection of metabolic mechanisms leading to insecticide resistance.
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Some studies have shown that mosquito larvae exposed to sub-lethal doses of pollutants, herbicides or pesticides display insecticide resistance through increased activities of insecticide-detoxifying enzymes.
“Some studies have also shown that mosquito larvae exposed to sub-lethal doses of pollutants, herbicides or pesticides display an insecticide resistance through increased activities of insecticide-detoxifying enzymes [26, 27].”
From Mapping insecticide resistance in Anopheles gambiae (s.l.) from Côte d'Ivoire (2018) · cites this paper as [27]
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- Authors
- Poupardin, R., Riaz, M. A., Jones, C. M., Chandor-Proust, A., Reynaud, S., & David, J.-P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Liverpool School of Tropical Medicine (GB)
- Corresponding author
- David, Jean-Philippe
- ORCID
- Venue
- Aquatic Toxicology
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 114-115
- Pages
- 49-57
- ISSN
- 0166-445X (ISSN-L)
- OpenAlex
- W1982823780
- Cited by
- 76
- References
- 44
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 5 · 2016: 2 · 2015: 6 · 2014: 6 · 2013: 9 · 2012: 3
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Aquatic Toxicology (publishedVersion)
- PubMed (publishedVersion)
- LSTM Online Archive (Liverpool School of Tropical Medicine) (submittedVersion)
- Funders
- Agence Nationale de la Recherche
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Social Sciences › Sociology and Political Science › Dengue and Mosquito Control Research
- Keywords
- insecticide resistance, permethrin, Aedes aegypti, fluoranthene, xenobiotics, mosquito larvae, gene selection, detoxification genes, cross-resistance, gene expression profiling, mosquito control
- MeSH terms
- Aedes, Animals, Biological Assay, Drug Interactions, Environmental Pollutants, Gene Expression Regulation, Insecticides, Larva, Selection, Genetic, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Permethrin, Protein Array Analysis
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