The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Development of Polydimethylsiloxane Substrates with Tunable Elastic Modulus to Study Cell Mechanobiology in Muscle and Nerve
Open accessAbstract
Mechanics is an important component in the regulation of cell shape, proliferation, migration and differentiation during normal homeostasis and disease states. Biomaterials that match the elastic modulus of soft tissues have been effective for studying this cell mechanobiology, but improvements are needed in order to investigate a wider range of physicochemical properties in a controlled manner. We hypothesized that polydimethylsiloxane (PDMS) blends could be used as the basis of a tunable system where the elastic modulus could be adjusted to match most types of soft tissue. To test this we formulated blends of two commercially available PDMS types, Sylgard 527 and Sylgard 184, which enabled us to fabricate substrates with an elastic modulus anywhere from 5 kPa up to 1.72 MPa. This is a three order-of-magnitude range of tunability, exceeding what is possible with other hydrogel and PDMS systems. Uniquely, the elastic modulus can be controlled independently of other materials properties including surface roughness, surface energy and the ability to functionalize the surface by protein adsorption and microcontact printing. For biological validation, PC12 (neuronal inducible-pheochromocytoma cell line) and C2C12 (muscle cell line) were used to demonstrate that these PDMS formulations support cell attachment and growth and that these substrates can be used to probe the mechanosensitivity of various cellular processes including neurite extension and muscle differentiation.
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Citations
Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
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Cell locomotion and focal adhesions are regulated by substrate flexibility
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Local force and geometry sensing regulate cell functions
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“However, the RMS roughness was ,1 nm for all the PDMS formulations, which is generally considered below the detectable range of cells [39].”
BackgroundControl of Stem Cell Fate by Physical Interactions with the Extracellular Matrix
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Myotubes differentiate optimally on substrates with tissue-like stiffness
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Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images
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Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
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“Other types of hydrogels have also been used over a similar stiffness range including synthetic systems based on polyethylene glycol [16] as well as naturallyderived polymers including hyaluron [17,18], methylcellulose [19], dextran [20], gelatin [21] and fibrin [22].”
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Cited by
Hinton, T. J., Jallerat, Q., Palchesko, R. N., Park, J. H., Grodzicki, M. S., Shue, H.-J., Ramadan, M. H., Hudson, A. R., & Feinberg, A. W. (2015) · Science Advances · doi:10.1126/sciadv.1500758 · Cited by 1,805
Self-citationPDMS with designer functionalities—Properties, modifications strategies, and applications
Wolf, M. P., Salieb-Beugelaar, G. B., & Hunziker, P. (2018) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2018.06.001 · Cited by 853
Wang, Z., Volinsky, A. A., & Gallant, N. D. (2014) · Journal of Applied Polymer Science · doi:10.1002/app.41050 · Cited by 522
Small molecule absorption by PDMS in the context of drug response bioassays
van Meer, B. J., de Vries, H., Firth, K. S. A., van Weerd, J., Tertoolen, L. G. J., Karperien, H. B. J., Jonkheijm, P., Denning, C., IJzerman, A. P., & Mummery, C. L. (2017) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.11.062 · Cited by 502
3D Printing PDMS Elastomer in a Hydrophilic Support Bath via Freeform Reversible Embedding
Hinton, T. J., Hudson, A., Pusch, K., Lee, A., & Feinberg, A. W. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00170 · Cited by 437
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Metadata
- Authors
- Palchesko, R. N., Zhang, L., Sun, Y., & Feinberg, A. W.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-12-11
- Volume
- 7
- Issue
- 12
- Pages
- e51499
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2060613028
- Cited by
- 559
- References
- 63
- FWCI
- 9.67
- Type
- journal article
- Language
- en
- Open access
- gold
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