Downregulation of yidC in Escherichia coli by Antisense RNA Expression Results in Sensitization to Antibacterial Essential Oils Eugenol and Carvacrol
Open accessAbstract
BACKGROUND: The rising drug resistance in pathogenic bacteria and inefficiency of current antibiotics to meet clinical requirements has augmented the need to establish new and innovative approaches for antibacterial drug discovery involving identification of novel antibacterial targets and inhibitors. Being obligatory for bacterial growth, essential gene products are considered vital as drug targets. The bacterial protein YidC is highly conserved among pathogens and is essential for membrane protein insertion due to which it holds immense potential as a promising target for antibacterial therapy. METHODS/PRINCIPAL FINDINGS: The aim of this study was to explore the feasibility and efficacy of expressed antisense-mediated gene silencing for specific downregulation of yidC in Escherichia coli. We induced RNA silencing of yidC which resulted in impaired growth of the host cells. This was followed by a search for antibacterial compounds sensitizing the YidC depleted cells as they may act as inhibitors of the essential protein or its products. The present findings affirm that reduction of YidC synthesis results in bacterial growth retardation, which warrants the use of this enzyme as a viable target in search of novel antibacterial agents. Moreover, yidC antisense expression in E. coli resulted in sensitization to antibacterial essential oils eugenol and carvacrol. Fractional Inhibitory Concentration Indices (FICIs) point towards high level of synergy between yidC silencing and eugenol/carvacrol treatment. Finally, as there are no known YidC inhibitors, the RNA silencing approach applied in this study put forward rapid means to screen novel potential YidC inhibitors. CONCLUSIONS/SIGNIFICANCE: The present results suggest that YidC is a promising candidate target for screening antibacterial agents. High level of synergy reported here between yidC silencing and eugenol/carvacrol treatment is indicative of a potential antibacterial therapy. This is the first report indicating that the essential gene yidC is a therapeutic target of the antibacterial essential oils eugenol and carvacrol in E. coli.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Decreasing YidC expression made E. coli more sensitive to the activity of carvacrol and eugenol.
“Patil et al. [91] explored the feasibility and efficacy of using antisense-mediated gene silencing to specifically down-regulate YidC in E. coli and found that decreasing YidC expression made the microorganism more sensitive to the activity of carvacrol and eugenol.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [91]
The essential gene YidC may represent a therapeutic target of the antibacterial essential oils eugenol and carvacrol.
“thus, the essential gene YidC may represent a therapeutic target of the antibacterial EOs eugenol and carvacrol.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [91]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488 · see citing works
Drugs for bad bugs: confronting the challenges of antibacterial discovery
Payne, D. J., Gwynn, M. N., Holmes, D. J., & Pompliano, D. L. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2201 · Cited by 2,512 · see citing works
Synergy, antagonism, and what the chequerboard puts between them
Odds, F. C. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg301 · Cited by 2,498 · see citing works
In vitro antibacterial activity of some plant essential oils
Prabuseenivasan, S., Jayakumar, M., & Savarimuthu Ignacimuthu, S. J. (2006) · BMC Complementary and Alternative Medicine · doi:10.1186/1472-6882-6-39 · Cited by 1,031 · see citing works
Trends in antimicrobial drug development: Implications for the future
Spellberg, B., Powers, J. H., Brass, E. P., Miller, L. G., & Edwards, J. E. (2004) · Clinical Infectious Diseases · doi:10.1086/420937 · Cited by 867 · see citing works
Platensimycin is a selective FabF inhibitor with potent antibiotic properties
Wang, J., Soisson, S. M., Young, K., Shoop, W. L., Kodali, S., Galgoci, A. M., Painter, R. E., Parthasarathy, G., Tang, Y. S., Cummings, R. T., et al. (2006) · Nature · doi:10.1038/nature04784 · Cited by 812 · see citing works
THE MECHANISM OF ACTION OF FOSFOMYCIN (PHOSPHONOMYCIN)
Kahan, F. M., Kahan, J. S., Cassidy, P. J., & Kropp, H. (1974) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1974.tb43277.x · Cited by 778 · see citing works
Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coliPreprint
Massé, É., Escorcia, F. E., & Gottesman, S. (2003) · Genes & Development · doi:10.1101/gad.1127103 · Cited by 705 · see citing works
Ouattara, B., SIMARD, R. E., Holley, R. A., Piette, G., & Bégin, A. (1997) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(97)00070-6 · Cited by 599 · see citing works
YidC mediates membrane protein insertion in bacteria.
Samuelson, J. C., Chen, M., Jiang, F., Möller, I., Wiedmann, M., Kuhn, A., Phillips, G. J., & Dalbey, R. E. (2000) · Nature · doi:10.1038/35020586 · Cited by 538 · see citing works
A genome‐wide strategy for the identification of essential genes in Staphylococcus aureus
Forsyth, A., Haselbeck, R. J., Ohlsen, K. L., Yamamoto, R. T., Xu, H. H., Trawick, J. D., Wall, D., Wang, L., Brown‐Driver, V., Froelich, J. M., et al. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02832.x · Cited by 488 · see citing works
Bactericidal antisense effects of peptide–PNA conjugates
Good, L., Awasthi, S. K., Dryselius, R., Larsson, O. S., & Nielsen, P. E. (2001) · Nature Biotechnology · doi:10.1038/86753 · Cited by 391 · see citing works
Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties
Wang, J., Kodali, S., Lee, S. H., Galgoci, A. M., Painter, R. E., Dorso, K., Racine, F., Motyl, M. R., Hernandez, L. D., Tinney, E., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700746104 · Cited by 379 · see citing works
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase.
Scotti, P. A., Urbanus, M. L., Brunner, J., de Gier, J. L., von Heijne, G., van der Does, C., Driessen, A. J. M., Oudega, B., & Luirink, J. (2000) · The EMBO Journal · doi:10.1093/emboj/19.4.542 · Cited by 374 · see citing works
Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering
Olano, C., Lombó, F., Méndez, C., & Salas, J. A. (2008) · Metabolic Engineering · doi:10.1016/j.ymben.2008.07.001 · Cited by 299 · see citing works
Antisense Properties of Duplex- and Triplex-Forming PNAs
Knudsen, H., & Nielsen, P. E. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.3.494 · Cited by 258 · see citing works
Gill, A. O., & Holley, R. A. (2006) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.04.046 · Cited by 226 · see citing works
Genetic Evidence for Inhibition of Bacterial Division Protein FtsZ by Berberine
Boberek, J. M., Stach, J., & Good, L. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013745 · Cited by 211 · see citing works
Escherichia coli YidC is a membrane insertase for Sec-independent proteins.
Serek, J., Bauer‐Manz, G., Struhalla, G., van den Berg, L., Kiefer, D., Dalbey, R., & Kuhn, A. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600063 · Cited by 211 · see citing works
Discovery of FabH/FabF Inhibitors from Natural Products
Young, K., Jayasuriya, H., Ondeyka, J. G., Herath, K. B., Zhang, C., Kodali, S., Galgoci, A. M., Painter, R. E., Brown‐Driver, V., Yamamoto, R., et al. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.50.2.519-526.2006 · Cited by 201 · see citing works
A conserved function of YidC in the biogenesis of respiratory chain complexes
van der Laan, M., Urbanus, M. L., ten Hagen‐Jongman, C. M., Nouwen, N. P., Oudega, B., Harms, N., Driessen, A. J. M., & Luirink, J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0636761100 · Cited by 154 · see citing works
Trends in antimicrobial drug development: Implications for the future
Sergio Mella, M. (2005) · Revista chilena de infectología · doi:10.4067/s0716-10182005000200011 · Cited by 143 · see citing works
Genetic strategies for antibacterial drug discovery
Miesel, L., Greene, J. R., & Black, T. A. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1086 · Cited by 141 · see citing works
DAVIES, J. E. (2007) · EMBO Reports · doi:10.1038/sj.embor.7401022 · Cited by 125 · see citing works
Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting
Pathania, R., Zlitni, S., Barker, C. A., Das, R., Gerritsma, D. A., Lebert, J. M., Awuah, E., Melacini, G., Capretta, F. A., & Brown, E. D. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.221 · Cited by 124 · see citing works
Bugs, drugs and chemical genomics
Roemer, T., DAVIES, J. E., Giaever, G. N., & Nislow, C. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.744 · Cited by 121 · see citing works
New (and not so new) antibacterial targets – from where and when will the novel drugs come?
Projan, S. J. (2002) · Current Opinion in Pharmacology · doi:10.1016/s1471-4892(02)00197-2 · Cited by 121 · see citing works
The Translation Start Codon Region Is Sensitive to Antisense PNA Inhibition in Escherichia coli
Dryselius, R., Aswasti, S. K., Rajarao, G. K., Nielsen, P. E., & Good, L. (2003) · Oligonucleotides · doi:10.1089/154545703322860753 · Cited by 118 · see citing works
Hasona, A., Crowley, P. J., Levesque, C. M., Mair, R. W., Cvitkovitch, D. G., Bleiweis, A. S., & Brady, L. J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0508778102 · Cited by 111 · see citing works
Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli
Nakashima, N., Tamura, T., & Good, L. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl697 · Cited by 107 · see citing works
Donald, R. G. K., Skwish, S., Forsyth, A., Anderson, J. W., Zhong, T., Burns, C. M., Lee, S., Meng, X., LoCastro, L., Jarantow, L. W., et al. (2009) · Chemistry & Biology · doi:10.1016/j.chembiol.2009.07.004 · Cited by 98 · see citing works
Ji, Y., Yin, D., Fox, B. A., Holmes, D. J., Payne, D. J., & Rosenberg, M. (2004) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(03)00931-5 · Cited by 76 · see citing works
Concurrent Growth Rate and Transcript Analyses Reveal Essential Gene Stringency in Escherichia coli
Goh, S., Boberek, J. M., Nakashima, N., Stach, J., & Good, L. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006061 · Cited by 73 · see citing works
Xu, H. H., Trawick, J. D., Haselbeck, R. J., Forsyth, A., Yamamoto, R. T., Archer, R., Patterson, J., Allen, M., Froelich, J. M., Taylor, I., et al. (2010) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00308-10 · Cited by 51 · see citing works
Antimicrobial synergy between mRNA- and protein-level inhibitors
Dryselius, R., Nekhotiaeva, N., & Good, L. (2005) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dki173 · Cited by 45 · see citing works
The evolution of YidC/Oxa/Alb3 family in the three domains of life: a phylogenomic analysis
Zhang, Y., Tian, H., & Wen, J. (2009) · BMC Evolutionary Biology · doi:10.1186/1471-2148-9-137 · Cited by 44 · see citing works
Vergidis, P. I., & Falagas, M. E. (2008) · PubMed · Cited by 43 · see citing works
Novel Antibacterials: A Genomics Approach to Drug Discovery
Chan, P. F., Macarrón, R., Payne, D. J., Zalacaín, M., & Holmes, D. J. (2002) · Current Drug Targets - Infectious Disorders · doi:10.2174/1568005023342227 · Cited by 42 · see citing works
Moir, D. T., Opperman, T. J., Butler, M. M., & Bowlin, T. L. (2012) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2012.07.004 · Cited by 40 · see citing works
Meng, J., Kanzaki, G., Meas, D., Lam, C. K., Crummer, H., Tain, J., & Xu, H. H. (2012) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2012.02503.x · Cited by 39 · see citing works
Variable coordination of cotranscribed genes in Escherichia coli following antisense repression.
Dryselius, R., Nikravesh, A., Kulyté, A., Goh, S., & Good, L. (2006) · BMC Microbiology · doi:10.1186/1471-2180-6-97 · Cited by 20 · see citing works
Pathania, R., & Brown, E. D. (2008) · Biochemistry and Cell Biology · doi:10.1139/o08-011 · Cited by 17 · see citing works
Kim, B. C., & Gu, M. B. (2006) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2005.11.032 · Cited by 10 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Effect of essential oils on pathogenic bacteria
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) · Pharmaceuticals · doi:10.3390/ph6121451 · Cited by 2,186 · see citing works
Bouyahya, A., Abrini, J., Dakka, N., & Bakri, Y. (2019) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2019.03.001 · Cited by 227 · see citing works
Friedman, M. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf5023862 · Cited by 203 · see citing works
Friedman, M. (2015) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5b00778 · Cited by 164 · see citing works
Application of Antibiotics/Antimicrobial Agents on Dental Caries
Qiu, W., Zhou, Y., Li, Z., Huang, T., Xiao, Y., Cheng, L., Peng, X., Zhang, L., & Ren, B. (2020) · BioMed Research International · doi:10.1155/2020/5658212 · Cited by 141 · see citing works
Strategies for target identification of antimicrobial natural products
Farha, M. A., & Brown, E. D. (2016) · Natural Product Reports · doi:10.1039/c5np00127g · Cited by 116 · see citing works
Dalbey, R., Kühn, A., Zhu, L., & Kiefer, D. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2013.12.022 · Cited by 100 · see citing works
Navani, N. K., Ghosh, Patil, D., Sharma, T., Srivastava, & Pathania, R. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s49649 · Cited by 100 · see citing works
Pichika, M. R., Mak, K., Kamal, M. B. B., Buru, A. S., Sakirolla, R., Kang, Y.-B., Mohandas, K., Balijepalli, M., & Ahmad, S. (2019) · Pharmacognosy Reviews/Bioinformatics Trends/Pharmacognosy review · doi:10.4103/phrev.phrev_46_18 · Cited by 89 · see citing works
Jensen, P. A., Zhu, Z., & van Opijnen, T. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.07.062 · Cited by 85 · see citing works
Antisense RNA: the new favorite in genetic research
Xu, J.-Z., Zhang, J.-L., & Zhang, W. (2018) · Journal of Zhejiang University SCIENCE B · doi:10.1631/jzus.b1700594 · Cited by 85 · see citing works
Co-translational protein targeting in bacteria.
Steinberg, R., Knüpffer, L., Origi, A., Asti, R., & Koch, H.-G. (2018) · FEMS Microbiology Letters · doi:10.1093/femsle/fny095 · Cited by 81 · see citing works
Bouyahya, A., Assemian, I. C. C., Mouzount, H., Bourais, I., Et-Touys, A., Fellah, H., Benjouad, A., Dakka, N., & Bakri, Y. (2018) · Industrial Crops and Products · doi:10.1016/j.indcrop.2018.11.001 · Cited by 76 · see citing works
Ahmad, A., Elisha, I. L., van Vuuren, S. F., & Viljoen, A. M. (2021) · Phytochemistry · doi:10.1016/j.phytochem.2021.112864 · Cited by 65 · see citing works
Antibiotic targeting of the bacterial secretory pathway
Smitha Rao, C. V., Waelheyns, E. D., Economou, A., & Anné, J. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2014.02.004 · Cited by 61 · see citing works
Alavi, M., & Rai, M. (2021) · Cellular Molecular and Biomedical Reports · doi:10.55705/cmbr.2021.142436.1014 · Cited by 49 · see citing works
Ghosh, T., Srivastava, S. K., Gaurav, A., Kumar, A., Kumar, P., Yadav, A. S., Pathania, R., & Navani, N. K. (2018) · Applied and Environmental Microbiology · doi:10.1128/aem.02487-18 · Cited by 47 · see citing works
Luong, A. D., Buzid, A., & Luong, J. H. T. (2022) · Journal of Functional Biomaterials · doi:10.3390/jfb13040175 · Cited by 32 · see citing works
Wu, S., Liu, Y., Zhang, H., & Lei, L. (2019) · Journal of Dental Sciences · doi:10.1016/j.jds.2019.09.006 · Cited by 24 · see citing works
Abdelhamid, S. M., Edris, A. E., & Sadek, Z. (2023) · Food Chemistry · doi:10.1016/j.foodchem.2023.135877 · Cited by 23 · see citing works
Strain engineering via regulatory noncoding RNAs: not a one-blueprint-fits-all
Cho, S. H., Haning, K., & Contreras, L. M. (2015) · Current Opinion in Chemical Engineering · doi:10.1016/j.coche.2015.07.008 · Cited by 21 · see citing works
Stress Conditions Induced by Carvacrol and Cinnamaldehyde on Acinetobacter baumannii
Montagu, A., Joly‐Guillou, M., Rossines, E., Cayon, J., Kempf, M., & Saulnier, P. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01133 · Cited by 21 · see citing works
Genomic tools to profile antibiotic mode of action
Cardona, S. T., Selin, C., & Gislason, A. S. (2014) · Critical Reviews in Microbiology · doi:10.3109/1040841x.2013.866073 · Cited by 19 · see citing works
Rapid and high-throughput construction of microbial cell-factories with regulatory noncoding RNAs
Chaudhary, A., Na, D., & Lee, E. Y. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.05.009 · Cited by 19 · see citing works
YidC as a potential antibiotic target
Dalbey, R., Kaushik, S., & Kühn, A. (2022) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2022.119403 · Cited by 14 · see citing works
Antimicrobial activities of microbial essential fatty acid against foodborne pathogenic bacteria
Firoozabad, M. S. M., & Mohammadinasr, M. (2022) · Iranian Journal of Microbiology · doi:10.18502/ijm.v14i2.9190 · Cited by 14 · see citing works
Suzuki, Y., Ishimoto, T., Fujita, S., Kiryu, S., Wada, M., Akatsuka, T., Saito, M., & Kawano, M. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.06.088 · Cited by 14 · see citing works
Yamamoto, K., Yamamoto, N., Ayukawa, S., Yasutake, Y., Ishiya, K., & Nakashima, N. (2022) · Scientific Reports · doi:10.1038/s41598-022-09635-6 · Cited by 14 · see citing works
Benmakhlouf, Z., Benserradj, O., & KELLAB, R. (2022) · Biodiversitas Journal of Biological Diversity · doi:10.13057/biodiv/d230540 · Cited by 11 · see citing works
Patil, S. D., Sharma, R., Bhattacharyya, T., Kumar, P., Gupta, M., Chaddha, B. S., Navani, N. K., & Pathania, R. (2015) · The Journal of Microbiology · doi:10.1007/s12275-015-4686-3 · Cited by 11 · see citing works
Tzeng, S., Huang, Y., Zhang, Y.-Q., Yang, C., Chien, H.-S., Chen, Y., Yu, S., Chen, C. S., & Chiu, H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21239312 · Cited by 11 · see citing works
Goodarzi, N. N., Asforooshani, M. K., Shahbazi, B., Rahimi, N. R., & Badmasti, F. (2024) · Frontiers in Bioinformatics · doi:10.3389/fbinf.2024.1482338 · Cited by 10 · see citing works
Legionella Pneumophila and Dendrimers-Mediated Antisense Therapy
Pashaei‐Asl, R., Khodadadi, K., Pashaei-Asl, F., Haqshenas, G., Ahmadian, N., Pashaiasl, M., & Baghdadabadi, R. H. H. (2017) · Advanced Pharmaceutical Bulletin · doi:10.15171/apb.2017.022 · Cited by 10 · see citing works
A Quest to the Therapeutic Arsenal: Novel Strategies to Combat Multidrug- resistant Bacteria
Ashwath, P., & Sannejal, A. D. (2021) · Current Gene Therapy · doi:10.2174/1566523221666210419084836 · Cited by 9 · see citing works
Kadeřábková, N., Mahmood, A. J. S., Furniss, R. C. D., & Mavridou, D. A. I. (2023) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/bs.ampbs.2023.05.003 · Cited by 8 · see citing works
The versatile role of YidC in membrane protein biosynthesis and quality control
Çalıseki, M., Schaffitzel, C., & Kabasakal, B. V. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2025.119956 · Cited by 7 · see citing works
Ashwath, P., & Sannejal, A. D. (2021) · Journal of Health and Allied Sciences NU · doi:10.1055/s-0041-1731914 · Cited by 7 · see citing works
Inhibition of Growth and Gene Expression in Staphylococcus Aureus by Anti- gyrA Peptide Nucleic Acid
Tavakoli, M., Hashemi, A., Vaezjalali, M., Mohammadzadeh, M., & Goudarzi, H. (2019) · Future Microbiology · doi:10.2217/fmb-2019-0103 · Cited by 5 · see citing works
Ashwath, P., Deekshit, V. K., Rohit, A., Rai, P., Aditya, V., Babu, N., Karunasagar, I., & Sannejal, A. D. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00585-y · Cited by 4 · see citing works
Antimicrobial Resistance: A Dentists’ Prospective
K. Swapna Kumari1, P. D. (2020) · Indian Journal of Forensic Medicine & Toxicology · doi:10.37506/ijfmt.v14i4.13018 · Cited by 2 · see citing works
Jensen, P. A., Zhu, Z., & van Opijnen, T. (2016) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/071704 · Cited by 1 · see citing works
Mahmoud, M. A. (2016) · ERA: Education and Research Archive (University of Alberta) · doi:10.7939/r3ft8dq31
Tong, X., Ferm, D. D., Liu, H., Wang, Y., Chandramouli, K., & Ganta, R. R. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.05.697664
Tong, X.-S., Ferm, D., Liu, H.-T., Wang, Y., Kondethimmanahalli, C., & Ganta, R. (2026) · Journal of Bacteriology · doi:10.1128/jb.00334-26
- Authors
- Patil, S. D., Sharma, R., Srivastava, S., Navani, N. K., & Pathania, R.
- Affiliations
- Indian Institute of Technology Roorkee (IN)
- Corresponding author
- Pathania, Ranjana
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-03-04
- Volume
- 8
- Issue
- 3
- Pages
- e57370
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3587592
- OpenAlex
- W1972232256
- Cited by
- 45
- References
- 43
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 4 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 4 · 2013: 2
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Department of Science and Technology, Ministry of Science and Technology, India
- Council of Scientific and Industrial Research, India
- Ministry of Education, India
- Royal Veterinary College
- Division of Human Resource Development
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- YidC, antisense RNA, gene silencing, eugenol, carvacrol, essential oils, synergistic antibacterial activity, membrane protein insertion, antibiotic targets, Escherichia coli, fractional inhibitory concentration index
- MeSH terms
- Cymenes, Anti-Bacterial Agents, Escherichia coli, Eugenol, Microbial Sensitivity Tests, Oils, Volatile, Colony Count, Microbial, Gene Expression Regulation, Bacterial, Genes, Essential, Gene Silencing, Membrane Transport Proteins, Escherichia coli Proteins, RNA, Small Interfering, Monoterpenes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
