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Rat Embryonic Mast Cells Originate in the AGM

Guiraldelli, Michel Farchi, França, Carolina Nunes, de Souza, Devandir Antonio, da Silva, Elaine Zayas Marcelino, Toso, Vanina Danuza, Carvalho, Celiane Cardoso, Jamur, Maria Célia, Oliver, Constance·2013·PLoS ONE
DOI 10.1371/journal.pone.0057862PMC PMC3591394
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Figure 1 Transversal section of aorta, gonads, and mesonephros region (AGM) of embryos at E11.5. (A) AGM region stained with Hematoxilin & Eosin (H.E) (B) Combined image of Differential Interference Contrast (DIC) and fluorescence microscopy showing a MCcp (arrow) labeled with mAb BGD6-FITC located near the inner face of the dorsal aorta wall. Inset: Clusters of MCcps are occasionally seen in the AGM.
Figure 1 Transversal section of aorta, gonads, and mesonephros region (AGM) of embryos at E11.5. (A) AGM region stained with Hematoxilin & Eosin (H.E) (B) Combined image of Differential Interference Contrast (DIC) and fluorescence microscopy showing a MCcp (arrow) labeled with mAb BGD6-FITC located near the inner face of the dorsal aorta wall. Inset: Clusters of MCcps are occasionally seen in the AGM.CC BY · PubMed Central
Figure 2 MCcps (AA4−/BGD6+) were immunomagnetically isolated from E11.5 rat embryos. After immunostaining, the cells were positive for the expression of CD34, c-kit and CD13. The secondary antibody was conjugated to FITC. Arrows, immunolabeled cells; arrowheads, magnetic beads conjugated to mAb BDG6.
Figure 2 MCcps (AA4−/BGD6+) were immunomagnetically isolated from E11.5 rat embryos. After immunostaining, the cells were positive for the expression of CD34, c-kit and CD13. The secondary antibody was conjugated to FITC. Arrows, immunolabeled cells; arrowheads, magnetic beads conjugated to mAb BDG6.CC BY · PubMed Central
Figure 3 RT-PCR analyses of the expression pattern of the alpha and beta subunits of FcεRI and mast cell specific proteases. Lane A. MCcp (AA4−/BGD6+) isolated at E11.5; Lane B. Adult rat bone marrow MCcp (AA4−/BGD6+); Lane C. Adult rat bone marrow mast cells (AA4+/BGD6+); Lane D. RBL-2H3 mast cells. FcεRIα: α subunit of the high affinity IgE receptor; FcεRIβ: β subunit of the high affinity IgE receptor; RMCP1: rat mast cell protease-1; RMCP5: rat mast cell protease-5; RCPA: rat carboxypeptidase A.
Figure 3 RT-PCR analyses of the expression pattern of the alpha and beta subunits of FcεRI and mast cell specific proteases. Lane A. MCcp (AA4−/BGD6+) isolated at E11.5; Lane B. Adult rat bone marrow MCcp (AA4−/BGD6+); Lane C. Adult rat bone marrow mast cells (AA4+/BGD6+); Lane D. RBL-2H3 mast cells. FcεRIα: α subunit of the high affinity IgE receptor; FcεRIβ: β subunit of the high affinity IgE receptor; RMCP1: rat mast cell protease-1; RMCP5: rat mast cell protease-5; RCPA: rat carboxypeptidase A.CC BY · PubMed Central
Figure 4 MCcps immunomagnetically isolated with mAb BGD6 from E11.5 embryos. (A) After 21 days in culture with SCF and IL-3 the MCcp grew as small colonies around the immunomagnetic beads (white arrows). (B) After 21 days in culture, many of the mast cells displayed metachromatic granules. Inset: A higher magnification of a metachromatic mast cell attached to a immunomagnetic bead. (Immunomagnetic beads, black arrows).
Figure 4 MCcps immunomagnetically isolated with mAb BGD6 from E11.5 embryos. (A) After 21 days in culture with SCF and IL-3 the MCcp grew as small colonies around the immunomagnetic beads (white arrows). (B) After 21 days in culture, many of the mast cells displayed metachromatic granules. Inset: A higher magnification of a metachromatic mast cell attached to a immunomagnetic bead. (Immunomagnetic beads, black arrows).CC BY · PubMed Central
Figure 5 Representative fields of liver from E12.5embryos. (A) At this stage, several mast cells are labeled with mAb BGD6 conjugated to FITC (arrows). (B) In contrast, only one immature mast cell (arrow) is immunolabeled with mAb AA4 conjugated to FITC.
Figure 5 Representative fields of liver from E12.5embryos. (A) At this stage, several mast cells are labeled with mAb BGD6 conjugated to FITC (arrows). (B) In contrast, only one immature mast cell (arrow) is immunolabeled with mAb AA4 conjugated to FITC.CC BY · PubMed Central
Figure 6 AA4−/BGD6+ and AA4+/BGD6+ cells were immunomagnetically isolated from dissociated embryos. With increasing days of gestation the percent of AA4−/BGD6+ mast cells decreases and the percent of AA4+/BGD6+ mast cells increases. Data is expressed as the mean±SD of 6 independent experiments.
Figure 6 AA4−/BGD6+ and AA4+/BGD6+ cells were immunomagnetically isolated from dissociated embryos. With increasing days of gestation the percent of AA4−/BGD6+ mast cells decreases and the percent of AA4+/BGD6+ mast cells increases. Data is expressed as the mean±SD of 6 independent experiments.CC BY · PubMed Central
Figure 7 From E13.5–19.5 mast cells colonize peripheral sites. Very immature mast cells (AA4+/BGD6+) could be identified by immunostaining in the caudal mesenchyme (A) at E13.5. At E15.5, the first metachromatic mast cells (arrows) appeared in the caudal mesenchyme (B). At E18.5 mast cells first appear in the bone marrow (C). At this time mast cells could only be identified in the bone marrow and in the liver (D) by immunostaining. A, C and D immunostained with mAb AA4-FITC. B, toluidine blue.
Figure 7 From E13.5–19.5 mast cells colonize peripheral sites. Very immature mast cells (AA4+/BGD6+) could be identified by immunostaining in the caudal mesenchyme (A) at E13.5. At E15.5, the first metachromatic mast cells (arrows) appeared in the caudal mesenchyme (B). At E18.5 mast cells first appear in the bone marrow (C). At this time mast cells could only be identified in the bone marrow and in the liver (D) by immunostaining. A, C and D immunostained with mAb AA4-FITC. B, toluidine blue.CC BY · PubMed Central

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Authors
Guiraldelli, M. F., França, C. N., de Souza, D. A., da Silva, E. Z. M., Toso, V. D., Carvalho, C. C., Jamur, M. C., & Oliver, C.
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-03-07
Volume
8
Issue
3
Pages
e57862
ISSN
1932-6203
DOI
10.1371/journal.pone.0057862
PMC
PMC3591394
Cited by
5
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)

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CoChat. (2026). Citation check: Rat Embryonic Mast Cells Originate in the AGM. https://app.cochat.ai/papers/10.1371/journal.pone.0057862
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Guiraldelli, M. F., França, C. N., de Souza, D. A., da Silva, E. Z. M., Toso, V. D., Carvalho, C. C., Jamur, M. C., & Oliver, C. (2013). Rat Embryonic Mast Cells Originate in the AGM. PLoS ONE. https://doi.org/10.1371/journal.pone.0057862