Cited by 108Tuberculosis Research and EpidemiologyImmune responses and vaccinationsImmunotherapy and Immune Responses

Enhanced Immune Response and Protective Effects of Nano-chitosan-based DNA Vaccine Encoding T Cell Epitopes of Esat-6 and FL against Mycobacterium Tuberculosis Infection

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Feng, Ganzhu, Jiang, Qingtao, Xia, Mei, Lu, Yanlai, Qiu, Wen, Zhao, Dan, Lu, Liwei, Peng, Guangyong +1 more·2013·PLoS ONE

Abstract

Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.

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  • A nano-chitosan-based recombinant DNA vaccine elevated the immunologic and protective effects against TB in a mouse model.

    “A nano-chitosan-based recombinant DNA vaccine elevated the immunologic and protective effects against TB in a mouse model 136”

    From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [136]

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Authors
Feng, G., Jiang, Q., Xia, M., Lu, Y., Qiu, W., Zhao, D., Lu, L., Peng, G., & Wang, Y.
Affiliations
  • Nanjing Medical University (CN)
  • University of Hong Kong (HK)
  • Saint Louis University (US)
Corresponding author
Wang, Yingwei
Venue
PLoS ONE
Published
2013-04-23
Volume
8
Issue
4
Pages
e61135
ISSN
1932-6203 (ISSN-L)
OpenAlex
W1968939141
Cited by
108
References
53
Citations by year
2026: 1 · 2025: 4 · 2024: 10 · 2023: 9 · 2022: 16 · 2021: 12 · 2020: 12 · 2019: 5 · 2018: 7 · 2017: 5 · 2016: 8 · 2015: 8 · 2014: 6 · 2013: 5
FWCI
6.57
Type
journal article
Language
en
Status
Retracted
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
Funders
  • Government of Jiangsu Province
  • Southeast University
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
Mycobacterium tuberculosis, tuberculosis vaccine, DNA vaccine, chitosan nanoparticles, ESAT-6, T cell epitopes, FMS-like tyrosine kinase 3 ligand, T cell response, protective immunity
MeSH terms
T-bet Transcription Factor, Amino Acid Sequence, Animals, Antigens, Bacterial, Bacterial Proteins, Cell Count, Female, Gene Expression Regulation, Immunization, Interferon-gamma, Ligands, Mice, Inbred C57BL, Molecular Sequence Data, Mycobacterium tuberculosis, Particle Size, Recombinant Proteins, Spleen, T-Lymphocytes, Cytotoxic, Transfection, Tuberculosis, Cytokines, Epitopes, T-Lymphocyte, Vaccines, DNA, T-Box Domain Proteins, Chitosan, Cell Proliferation, GATA3 Transcription Factor, Mice, fms-Like Tyrosine Kinase 3, Nanoparticles

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