The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae
Open accessAbstract
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into the genome by homologous recombination. However, the available yeast tagging plasmids have not kept pace with the development of new and improved fluorescent proteins. Here, we have constructed yeast optimized versions of 19 different fluorescent proteins and tested them for use as fusion tags in yeast. These include two blue, seven green, and seven red fluorescent proteins, which we have assessed for brightness, photostability and perturbation of tagged proteins. We find that EGFP remains the best performing green fluorescent protein, that TagRFP-T and mRuby2 outperform mCherry as red fluorescent proteins, and that mTagBFP2 can be used as a blue fluorescent protein tag. Together, the new tagging vectors we have constructed provide improved blue and red fluorescent proteins for yeast tagging and three color imaging.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Coding-Sequence Determinants of Gene Expression in Escherichia coli
Kudla, G., Murray, A. W., Tollervey, D., & Plotkin, J. B. (2009) · Science · doi:10.1126/science.1170160 · Cited by 1,458
Improving FRET dynamic range with bright green and red fluorescent proteins
Lam, A. J., St-Pierre, F., Gong, Y., Marshall, J. D., Cranfill, P. J., Baird, M. A., McKeown, M. R., Wiedenmann, J., Davidson, M. W., Schnitzer, M. J., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2171 · Cited by 732
Cited by
Phototoxicity in live fluorescence microscopy, and how to avoid it
Icha, J., Weber, M., Waters, J. C., & Norden, C. (2017) · BioEssays · doi:10.1002/bies.201700003 · Cited by 622
Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling
Williams, C. C., Jan, C. H., & Weissman, J. S. (2014) · Science · doi:10.1126/science.1257522 · Cited by 391
Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis
Chong, Y. T., Koh, J. L. Y., Friesen, H., Kaluarachchi Duffy, S., Cox, M. J., Moses, A., Moffat, J., Boone, C., & Andrews, B. J. (2015) · Cell · doi:10.1016/j.cell.2015.04.051 · Cited by 274
Metadata
- Authors
- Lee, S., Lim, W. A., & Thorn, K. S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-07-02
- Volume
- 8
- Issue
- 7
- Pages
- e67902
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2064995454
- Cited by
- 210
- References
- 3
- FWCI
- 6.64
- Type
- journal article
- Language
- en
- Open access
- gold
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