The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Functional Characterization of Bacteria Isolated from Ancient Arctic Soil Exposes Diverse Resistance Mechanisms to Modern Antibiotics
Open accessAbstract
Using functional metagenomics to study the resistomes of bacterial communities isolated from different layers of the Canadian high Arctic permafrost, we show that microbial communities harbored diverse resistance mechanisms at least 5,000 years ago. Among bacteria sampled from the ancient layers of a permafrost core, we isolated eight genes conferring clinical levels of resistance against aminoglycoside, β-lactam and tetracycline antibiotics that are naturally produced by microorganisms. Among these resistance genes, four also conferred resistance against amikacin, a modern semi-synthetic antibiotic that does not naturally occur in microorganisms. In bacteria sampled from the overlaying active layer, we isolated ten different genes conferring resistance to all six antibiotics tested in this study, including aminoglycoside, β-lactam and tetracycline variants that are naturally produced by microorganisms as well as semi-synthetic variants produced in the laboratory. On average, we found that resistance genes found in permafrost bacteria conferred lower levels of resistance against clinically relevant antibiotics than resistance genes sampled from the active layer. Our results demonstrate that antibiotic resistance genes were functionally diverse prior to the anthropogenic use of antibiotics, contributing to the evolution of natural reservoirs of resistance genes.
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Cites
Wiegand, I., Hilpert, K., & Hancock, R. E. W. (2008) · Nature Protocols · doi:10.1038/nprot.2007.521 · Cited by 6,156
Identification of acquired antimicrobial resistance genes
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Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Salter, S. J., Cox, M. J., Turek, E. M., Calus, S. T., Cookson, W. O., Moffatt, M. F., Turner, P., Parkhill, J., Loman, N. J., & Walker, A. W. (2014) · BMC Biology · doi:10.1186/s12915-014-0087-z · Cited by 3,463
Antibiotic resistance is ancient
D’Costa, V. M., King, C. E., Kalan, L., Morar, M., Sung, W. W. L., Schwarz, C., Froese, D., Zazula, G., Calmels, F., Debruyne, R., et al. (2011) · Nature · doi:10.1038/nature10388 · Cited by 2,720
The shared antibiotic resistome of soil bacteria and human pathogens
Forsberg, K. J., Reyes, A., Wang, B., Selleck, E. M., Sommer, M. O. A., & Dantas, G. (2012) · Science · doi:10.1126/science.1220761 · Cited by 1,650
Self-citationSampling the Antibiotic Resistome
D'Costa, V. M., McGrann, K. M., Hughes, D. W., & Wright, G. D. (2006) · Science · doi:10.1126/science.1120800 · Cited by 1,594
Functional characterization of the antibiotic resistance reservoir in the human microflora
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Self-citationAntibiotic resistance is prevalent in an isolated cave microbiome
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Cited by
Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer
von Wintersdorff, C. J. H., Penders, J., van Niekerk, J. M., Mills, N. D., Majumder, S., van Alphen, L. B., Savelkoul, P. H. M., & Wolffs, P. F. G. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00173 · Cited by 1,707
Permafrost carbon emissions in a changing Arctic
Miner, K. R., Turetsky, M. R., Malina, E., Bartsch, A., Tamminen, J., McGuire, A. D., Fix, A., Sweeney, C., Elder, C. D., & Miller, C. E. (2022) · Nature Reviews Earth & Environment · doi:10.1038/s43017-021-00230-3 · Cited by 538
Roberts, M. C., & Schwarz, S. (2016) · Journal of Environmental Quality · doi:10.2134/jeq2015.04.0207 · Cited by 192
Hamilton, K. A., Garner, E., Joshi, S., Ahmed, W., Ashbolt, N., Medema, G., & Pruden, A. (2020) · Current Opinion in Environmental Science & Health · doi:10.1016/j.coesh.2020.02.012 · Cited by 32
Bell, G. (2016) · Proceedings of the Royal Society B: Biological Sciences · doi:10.1098/rspb.2015.2547 · Cited by 15
Metadata
- Authors
- Perron, G. G., Whyte, L., Turnbaugh, P. J., Goordial, J., Hanage, W. P., Dantas, G., & Desai, M. M.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-03-25
- Volume
- 10
- Issue
- 3
- Pages
- e0069533
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2142304411
- Cited by
- 229
- References
- 8
- FWCI
- 6.64
- Type
- journal article
- Language
- en
- Open access
- gold
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- Perron, G. G., Whyte, L., Turnbaugh, P. J., Goordial, J., Hanage, W. P., Dantas, G., & Desai, M. M. (2015). Functional Characterization of Bacteria Isolated from Ancient Arctic Soil Exposes Diverse Resistance Mechanisms to Modern Antibiotics. PLOS ONE. https://doi.org/10.1371/journal.pone.0069533
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