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Strategy for Sensitive and Specific Detection of Yersinia pestis in Skeletons of the Black Death Pandemic

Seifert, Lisa, Harbeck, Michaela, Thomas, Astrid, Hoke, Nadja, Zöller, Lothar, Wiechmann, Ingrid, Grupe, Gisela, Scholz, Holger C., Riehm, Julia M.·2013·PLoS ONE
DOI 10.1371/journal.pone.0075742PMC PMC3775804
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Figure 1 Schematic workflow for the proceeding through highly optimized and validated protocols for the detection of Y. pestis from ancient samples.
Figure 1 Schematic workflow for the proceeding through highly optimized and validated protocols for the detection of Y. pestis from ancient samples.CC BY · PubMed Central
Figure 2 Amino acid profile of specimen MP 59-I, channel 1 (570 nm) and 2 (440 nm). The profile represents a typical collagenous pattern with a high amino acid yield. However, aspartic and glutamic acid concentrations are significantly reduced. At 72 min an unidentified peak was detected that did not show up in the other samples.
Figure 2 Amino acid profile of specimen MP 59-I, channel 1 (570 nm) and 2 (440 nm). The profile represents a typical collagenous pattern with a high amino acid yield. However, aspartic and glutamic acid concentrations are significantly reduced. At 72 min an unidentified peak was detected that did not show up in the other samples.CC BY · PubMed Central
Figure 3 Conventional PCR targeting a 133 bp fragment of Y. pestis pla gene. A) Amplification products of the synthetic pla construct at concentrations of 10, 5 and 1 copy, respectively, are shown. Three samples were extracted from ancient bones originating from a modern time cemetery lacking any Y. pestis specific amplicons (bone matrix). B) Besides the synthetic pla construct, the gel shows products of PCR reactions set up, containing 1 ng DNA of each from the negative panel organisms (Table 2). C) Gel showing amplification products of aDNA extracted from teeth (Table 1). M: marker, PC: positive control, NTC: no template control, MMC: master mix control, WC: water control, MP: Manching-Pichl, Germany, origin of individuals, E1-8: extraction controls, Ba: Basel, Switzerland, origin of individuals, B: Brandenburg, Germany, origin of individuals.
Figure 3 Conventional PCR targeting a 133 bp fragment of Y. pestis pla gene. A) Amplification products of the synthetic pla construct at concentrations of 10, 5 and 1 copy, respectively, are shown. Three samples were extracted from ancient bones originating from a modern time cemetery lacking any Y. pestis specific amplicons (bone matrix). B) Besides the synthetic pla construct, the gel shows products of PCR reactions set up, containing 1 ng DNA of each from the negative panel organisms (Table 2). C) Gel showing amplification products of aDNA extracted from teeth (Table 1). M: marker, PC: positive control, NTC: no template control, MMC: master mix control, WC: water control, MP: Manching-Pichl, Germany, origin of individuals, E1-8: extraction controls, Ba: Basel, Switzerland, origin of individuals, B: Brandenburg, Germany, origin of individuals.CC BY · PubMed Central
Figure 4 qPCR targeting Y. pestis pla gene screening ancient samples.
Figure 4 qPCR targeting Y. pestis pla gene screening ancient samples.CC BY · PubMed Central

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Cited by

Metadata

Authors
Seifert, L., Harbeck, M., Thomas, A., Hoke, N., Zöller, L., Wiechmann, I., Grupe, G., Scholz, H. C., & Riehm, J. M.
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-09-17
Volume
8
Issue
9
Pages
e75742
ISSN
1932-6203
DOI
10.1371/journal.pone.0075742
PMC
PMC3775804
Cited by
18
References
4
Language
en
Update notices
correction (2013-10-21)
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)

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