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Cited by 104Oral microbiology and periodontitis researchPeriodontal Regeneration and TreatmentsOral and gingival health research

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Quantitative Proteomic Analysis of Gingival Crevicular Fluid in Different Periodontal Conditions

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Silva-Boghossian, Carina M., Colombo, Ana Paula V., Tanaka, Marcia, Rayo, Carolina, Xiao, Yizhi, Siqueira, Walter L.·2013·PLoS ONE
DOI 10.1371/journal.pone.0075898PMC PMC3787961
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Abstract

AIM: To quantify the proteome composition of the GCF in periodontal health (HH) and in sites with different clinical conditions in chronic periodontitis (CP) subjects. MATERIAL AND METHODS: 5 subjects with HH and 5 with CP were submitted to full-mouth periodontal examination, and GCF sampling. Sites in the CP group were classified and sampled as periodontitis (P, probing depth, PD>4 mm), gingivitis (G, PD≤3 mm with bleeding on probing, BOP), and healthy sites (H, PD≤3 mm without BOP). GCF proteins were subjected to liquid chromatography electrospray ionization mass spectrometry for identification, characterization and quantification. RESULTS: 230 proteins were identified; 145 proteins were detected in HH, 214 in P, 154 in G, and 133 in H. Four proteins were exclusively detected at HH, 43 proteins at P, 7 proteins at G, and 1 protein at H. Compared to HH group, 35 and 6 proteins were more abundant in P and G (p<0.001), respectively; and 4, 15 and 37 proteins were less abundant in P, G and H (p≤0.01), respectively. CONCLUSIONS: There are marked differences in the GCF proteome according to disease profile. Comprehension of the role of the identified proteins in the etiopathogenesis of periodontal disease may lead to biomarkers definition.

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Figure 1 Examples of base-peak chromatograms of the clinical groups. Peptide separation was achieved using a nano-flow reverse-phase HPLC column, with gradient elution ranging from 5 to 55% solvent B in 100 min. P: sites with probing depth >4 mm; G: sites with probing depth ≤3 mm and bleeding on probing; H: sites with probing depth ≤3 mm without bleeding on probing in periodontitis subjects; HH: sites with probing depth ≤3 mm without bleeding on probing in healthy subjects.
Figure 1 Examples of base-peak chromatograms of the clinical groups. Peptide separation was achieved using a nano-flow reverse-phase HPLC column, with gradient elution ranging from 5 to 55% solvent B in 100 min. P: sites with probing depth >4 mm; G: sites with probing depth ≤3 mm and bleeding on probing; H: sites with probing depth ≤3 mm without bleeding on probing in periodontitis subjects; HH: sites with probing depth ≤3 mm without bleeding on probing in healthy subjects.CC BY · PubMed Central
Figure 2 Venn diagram summarizing the absolute number of proteins detected in gingival crevicular fluid samples from periodontally healthy (145 proteins), and chronic periodontitis subjects in three categories P (deep probing depth sites, 214 proteins), G (shallow probing depth sites with bleeding on probing, 154 proteins) and H (shallow sites without bleeding on probing, 133 proteins).
Figure 2 Venn diagram summarizing the absolute number of proteins detected in gingival crevicular fluid samples from periodontally healthy (145 proteins), and chronic periodontitis subjects in three categories P (deep probing depth sites, 214 proteins), G (shallow probing depth sites with bleeding on probing, 154 proteins) and H (shallow sites without bleeding on probing, 133 proteins).CC BY · PubMed Central
Figure 3 ELISA experiment with 10 μg of GCF material for each group and anti-lysozyme antibody. Bars represent standard deviation of the mean, calculated from three independent experiments. Different lower case letters denote statistical difference according to Analysis of variance and Student-Newman-Keels’ test. P: sites with probing depth >4 mm; G: sites with probing depth ≤3 mm and bleeding on probing; H: sites with probing depth ≤3 mm without bleeding on probing in periodontitis subjects; HH: sites with probing depth ≤3 mm without bleeding on probing in healthy subjects.
Figure 3 ELISA experiment with 10 μg of GCF material for each group and anti-lysozyme antibody. Bars represent standard deviation of the mean, calculated from three independent experiments. Different lower case letters denote statistical difference according to Analysis of variance and Student-Newman-Keels’ test. P: sites with probing depth >4 mm; G: sites with probing depth ≤3 mm and bleeding on probing; H: sites with probing depth ≤3 mm without bleeding on probing in periodontitis subjects; HH: sites with probing depth ≤3 mm without bleeding on probing in healthy subjects.CC BY · PubMed Central

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Research works printed but not linked: 1

  1. WL Siqueira, 2012, Quantitative proteomic analysis of the effect of fluoride on the acquired enamel pellicle, PLOS ONE, 7, e42204, 10.1371/journal.pone.0042204 · DOI

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Metadata

Authors
Silva-Boghossian, C. M., Colombo, A. P. V., Tanaka, M., Rayo, C., Xiao, Y., & Siqueira, W. L.
Affiliations
  • Universidade Federal do Rio de Janeiro (BR)
  • Western University (CA)
Corresponding author
Siqueira, Walter L.
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-10-01
Volume
8
Issue
10
Pages
e75898
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0075898
PMC
PMC3787961
OpenAlex
W1965316932
Cited by
104
References
31
Citations by year
2026: 4 · 2025: 9 · 2024: 6 · 2023: 10 · 2022: 5 · 2021: 16 · 2020: 22 · 2019: 4 · 2018: 6 · 2017: 10 · 2016: 4 · 2015: 3 · 2014: 4 · 2013: 1
FWCI
4.85
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Canadian Institutes of Health Research (25116, 113166, 106657, 97577)
  • Natural Sciences and Engineering Research Council of Canada (#371813, 113166)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
  • Health Sciences › Dentistry › Periodontics › Oral and gingival health research
Keywords
gingival crevicular fluid, proteome, chronic periodontitis, gingivitis, periodontal health, periodontal biomarkers, protein abundance
MeSH terms
Female, Gingival Crevicular Fluid, Humans, Male, Middle Aged, Case-Control Studies, Proteomics, Chronic Periodontitis, Young Adult

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