Mass Spectrometric Analyses of Peptides and Proteins in Human Gingival Crevicular Fluid
Abstract
Gingival crevicular fluid (GCF) is a pathophysiological fluid that flows into the oral cavity. Human GCF was collected using sterile glass microcapillary tubes from inflamed periodontal sites in patients who had a history of periodontal disease and were in the maintenance phase of treatment. Samples from individual sites were analyzed using MS techniques both before and following HPLC. GCF samples were also pooled and subjected to SDS-PAGE, in-gel digestion and MS analyses using both MALDI-TOF/TOF MS and nanoLC-ESI-MS/MS. MS spectra were used to search human protein sequence databases for protein identification. With these approaches, 33 peptides and 66 proteins were positively identified in human GCF. All of the peptides discovered in this study are reported in GCF here for the first time. Forty-three of the identified proteins, such as actin and the actin binding proteins profilin, cofilin and gelsolin, have not been reported in GCF before.
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Culture-independent methods have improved our understanding of microbial diversity in the oral cavity.
“Culture‐independent methods have improved our understanding of microbial diversity in the oral cavity.”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [69]
Cites
Neutrophil Extracellular Traps Kill Bacteria
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y., & Zychlinsky, A. (2004) · Science · doi:10.1126/science.1092385 · Cited by 10,268 · see citing works
Periodontal Disease in Pregnancy II. Correlation Between Oral Hygiene and Periodontal Condition
Silness, J., & Löe, H. (1964) · Acta Odontologica Scandinavica · doi:10.3109/00016356408993968 · Cited by 7,932 · see citing works
Microbial complexes in subgingival plaque
Socransky, S. S., Haffajee, A. D., Cugini, M. A., Smith, C., & Kent, R. L. (1998) · Journal of Clinical Periodontology · doi:10.1111/j.1600-051x.1998.tb02419.x · Cited by 5,050 · see citing works
Pathogenesis of inflammatory periodontal disease. A summary of current work.
Rc, P., & Schroeder, H. E. (1976) · PubMed · Cited by 1,102 · see citing works
Characterization of the Low Molecular Weight Human Serum Proteome
Tirumalai, R. S., Chan, K. C., Prieto, D. A., Issaq, H. J., Conrads, T. P., & Veenstra, T. D. (2003) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m300031-mcp200 · Cited by 846 · see citing works
Activities of LL-37, a Cathelin-Associated Antimicrobial Peptide of Human Neutrophils
Turner, J., Cho, Y., Dinh, N.-N., Waring, A. J., & Lehrer, R. I. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.9.2206 · Cited by 800 · see citing works
Agerberth, B., Charo, J., Werr, J., Olsson, B., Idali, F., Lindbom, L., Kiessling, R., Jörnvall, H., Wigzell, H., & Gudmundsson, G. H. (2000) · Blood · doi:10.1182/blood.v96.9.3086 · Cited by 758 · see citing works
Absence of bleeding on probing An indicator of periodontal stability
Lang, N. P., Adler, R., Joss, A., & Nyman, S. (1990) · Journal of Clinical Periodontology · doi:10.1111/j.1600-051x.1990.tb01059.x · Cited by 671 · see citing works
Mørtz, E., Krogh, T. N., Vorum, H., & Görg, A. (2001) · PROTEOMICS · doi:10.1002/1615-9861(200111)1:11 · Cited by 519 · see citing works
Role of bacterial proteinases in matrix destruction and modulation of host responses
POTEMPA, J., BANBULA, A., & TRAVIS, J. (2000) · Periodontology 2000 · doi:10.1034/j.1600-0757.2000.2240108.x · Cited by 382 · see citing works
Hu, S., Xie, Y., Ramachandran, P., Loo, R. R. O., Li, Y., Loo, J. A., & Wong, D. T. (2005) · PROTEOMICS · doi:10.1002/pmic.200401037 · Cited by 361 · see citing works
Mäkelä, M., Salo, T. A., Uitto, V. J., & Larjava, H. (1994) · Journal of Dental Research · doi:10.1177/00220345940730080201 · Cited by 325 · see citing works
Identification of human whole saliva protein components using proteomics
Vitorino, R. M. P., Calheiros‐Lobo, M. J., Ferrer‐Correira, A. J., Dubin, J. R., Tomer, K. B., Domingues, P. M., & Amado, F. M. L. (2004) · PROTEOMICS · doi:10.1002/pmic.200300638 · Cited by 295 · see citing works
Nagaoka, I., Tamura, H., & Hirata, M. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.5.3044 · Cited by 273 · see citing works
<b>Apatite-mediated Actin Dynamics in Resorbing Osteoclasts</b>
Saltel, F., Destaing, O., Bard, F., Eichert, D., & Jurdic, P. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-06-0522 · Cited by 267 · see citing works
Antimicrobial and protease inhibitory functions of the human cathelicidin (hCAP18/LL-37) prosequence
Zaiou, M., Nizet, V., & Gallo, R. L. (2003) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2003.12132.x · Cited by 256 · see citing works
Claffey, N. M., Nylund, K., Kiger, R., Garrett, S., & Egelberg, J. H. (1990) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1990.tb01071.x · Cited by 231 · see citing works
Badersten, A., Nilvéus, R., & Egelberg, J. H. (1990) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1990.tb01070.x · Cited by 218 · see citing works
Koomen, J. M., Li, D., Xiao, L.-C., Liu, T. C., Coombes, K. R., Abbruzzese, J. L., & Kobayashi, R. (2005) · Journal of Proteome Research · doi:10.1021/pr050046x · Cited by 216 · see citing works
Antihypertensive peptides derived from food proteins
Yamamoto, N. (1997) · Biopolymers · doi:10.1002/(sici)1097-0282(1997)43:2 · Cited by 210 · see citing works
Salivary Antimicrobial Peptide Expression and Dental Caries Experience in Children
Tao, R., Jurevic, R. J., Coulton, K. M., Tsutsui, M. T., Roberts, M. C., Kimball, J. R., Wells, N. J., Berndt, J., & Dale, B. A. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.9.3883-3888.2005 · Cited by 208 · see citing works
Comparative proteomic analysis of human whole saliva
Huang, C. (2004) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2004.06.003 · Cited by 193 · see citing works
Composition of the peptide fraction in human blood plasma: database of circulating human peptides
Richter, R., Schulz‐Knappe, P., Schrader, M., Ständker, L., Jürgens, M., Tammen, H., & Forssmann, W. (1999) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(99)00012-2 · Cited by 188 · see citing works
Hardt, M., Thomas, L. R., Dixon, S. E., Newport, G., Agabian, N. M., Prakobphol, A., Hall, S. C., Witkowska, H. E., & Fisher, S. J. (2005) · Biochemistry · doi:10.1021/bi048176r · Cited by 167 · see citing works
Indicators of periodontal disease activity: an evaluation
Fine, D. H., & Mandel, I. D. (1986) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1986.tb01502.x · Cited by 167 · see citing works
Extracellular neutrophil traps in periodontitis
Vitkov, L., Klappacher, M., Hannig, M., & Krautgartner, W. D. (2009) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.2008.01175.x · Cited by 154 · see citing works
Walz, A., Stühler, K., Wattenberg, A., Hawranke, E., Meyer, H. E., Schmalz, G., Blüggel, M., & Rühl, S. (2006) · PROTEOMICS · doi:10.1002/pmic.200500125 · Cited by 146 · see citing works
Human hemoglobin-derived peptides exhibit antimicrobial activity: a class of host defense peptides
Liepke, C., Baxmann, S., Heine, C., Breithaupt, N., Ständker, L., & Forssmann, W. (2003) · Journal of Chromatography B · doi:10.1016/s1570-0232(03)00245-9 · Cited by 145 · see citing works
Nisapakultorn, K., Ross, K. F., & Herzberg, M. C. (2001) · Infection and Immunity · doi:10.1128/iai.69.7.4242-4247.2001 · Cited by 136 · see citing works
The Correlation of Gingivitis Pocket Depth and Exudate from the Gingival Crevice
Mann, W. V. (1963) · Journal of Periodontology · doi:10.1902/jop.1963.34.4.379 · Cited by 125 · see citing works
New antibacterial peptide derived from bovine hemoglobin
Daoud, R., Dubois, V., Bors-Dodita, L., Nedjar‐Arroume, N., Krier, F., Chihib, N., Mary, P., Kouach, M., Briand, G., & Guillochon, D. (2005) · Peptides · doi:10.1016/j.peptides.2004.12.008 · Cited by 124 · see citing works
Kinane, D. F., Darby, I. B., Said, S., Luoto, H., Sorsa, T. A., Tikanoja, S., & Mäntylä, P. (2003) · Journal of Periodontal Research · doi:10.1034/j.1600-0765.2003.00663.x · Cited by 117 · see citing works
Self-citationPardi, A., Zhang, X. L., Selsted, M. E., Skalicky, J. J., & Yip, P. F. (1992) · Biochemistry · doi:10.1021/bi00161a013 · Cited by 115 · see citing works
Experimental gingivitis in young dogs
Lindhe, J., & Rylander, H. (1975) · European Journal Of Oral Sciences · doi:10.1111/j.1600-0722.1975.tb00444.x · Cited by 114 · see citing works
Nakashima, K., Giannopoulou, C., Andersen, E., Roehrich, N., Brochut, P., Dubrez, B., & Cimasoni, G. (1996) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1996.tb00620.x · Cited by 108 · see citing works
Lamster, I. B., Hartley, L. J., & Vogel, R. I. (1985) · Journal of Periodontology · doi:10.1902/jop.1985.56.11s.13 · Cited by 108 · see citing works
Kojima, T., Andersen, E., Sanchez, J., Wilkins, M. R., Hochstrasser, D. F., Pralong, W. F., & Cimasoni, G. (2000) · Journal of Dental Research · doi:10.1177/00220345000790020701 · Cited by 105 · see citing works
Alpagot, T., Bell, C., Lundergan, W. P., Chambers, D. W., & Rudin, R. (2001) · Journal Of Clinical Periodontology · doi:10.1034/j.1600-051x.2001.028004353.x · Cited by 105 · see citing works
Gingival crevicular fluid gelatinase and its relationship to periodontal disease in human subjects
Teng, Y. T., Sodek, J., & McCulloch, C. A. (1992) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1992.tb01830.x · Cited by 100 · see citing works
Giannobile, W. V., Lynch, S. E., Denmark, R. G., Paquette, D. W., Fiorellini, J. P., & Williams, R. C. (1995) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1995.tb01793.x · Cited by 97 · see citing works
Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS.
Pisano, E., Cabras, T., Montaldo, C., Piras, V., Inzitari, R., Olmi, C., Castagnola, M., & Messana, I. (2005) · European Journal of Oral Sciences · doi:10.1111/j.1600-0722.2005.00246.x · Cited by 95 · see citing works
Gingival Crevicular Fluid: Biomarkers of Periodontal Tissue Activity
Embery, G., & Waddington, R. J. (1994) · Advances in Dental Research · doi:10.1177/08959374940080022901 · Cited by 82 · see citing works
Chapple, I., Garner, I., Saxby, M. S., Moscrop, H., & Matthews, J. B. (1999) · Journal Of Clinical Periodontology · doi:10.1034/j.1600-051x.1999.260310.x · Cited by 74 · see citing works
Diagnosis of periodontal disease based on analysis of the host response
Lamster, I. B., & Grbic, J. T. (1995) · Periodontology 2000 · doi:10.1111/j.1600-0757.1995.tb00038.x · Cited by 74 · see citing works
Antibacterial hemoglobin peptides in human menstrual blood
Mak, P., Wójcik, K., Wicherek, Ł., Suder, P., & Dubin, A. (2004) · Peptides · doi:10.1016/j.peptides.2004.06.015 · Cited by 66 · see citing works
Petropoulos, G., McKay, I. J., & Hughes, F. J. (2004) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2004.00489.x · Cited by 61 · see citing works
Curtis, M. A., Sterne, J. A. C., Price, S. J., Griffiths, G. S., Coulthurst, S. K., Wilton, J. M. A., & Johnson, N. W. (1990) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1990.tb01202.x · Cited by 58 · see citing works
Palys, M. D., Haffajee, A. D., Socransky, S. S., & Giannobile, W. V. (1998) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1998.tb02383.x · Cited by 55 · see citing works
Antimicrobial peptides derived from heme-containing proteins: Hemocidins
Mak, P., Wójcik, K., Silberring, J., & Dubin, A. (2000) · Antonie van Leeuwenhoek · doi:10.1023/a:1002081605784 · Cited by 54 · see citing works
Talonpoika, J. T., & Hämäläinen, M. M. (1994) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1994.tb00720.x · Cited by 53 · see citing works
Lundy, F. T., ORR, D., Shaw, C., Lamey, P. ‐., & Linden, G. J. (2004) · Molecular Immunology · doi:10.1016/j.molimm.2004.09.008 · Cited by 51 · see citing works
Proteolytic degradation of hemoglobin in erythrocytes leads to biologically active peptides
Karelin, A. A., Philippova, M. M., & Ivanov, V. T. (1995) · Peptides · doi:10.1016/0196-9781(95)00029-j · Cited by 50 · see citing works
Calcitonin gene‐related peptide in gingival crevicular fluid in periodontal health and disease
Lundy, F. T., Shaw, C., McKinnell, J., Lamey, P. J., & Linden, G. J. (1999) · Journal Of Clinical Periodontology · doi:10.1034/j.1600-051x.1999.260403.x · Cited by 50 · see citing works
Adonogianaki, E., Mooney, J. J., & Kinane, D. F. (1996) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1996.tb00475.x · Cited by 44 · see citing works
Nakashima, K., Demeurisse, C., & Cimasoni, G. (1994) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1994.tb00411.x · Cited by 41 · see citing works
Chang, K.-M., Lehrhaupt, N. B., Lin, L. M., Feng, J., Wu-Wang, C.-Y., & Wang, S.-L. (1996) · Archives of Oral Biology · doi:10.1016/s0003-9969(96)00024-6 · Cited by 39 · see citing works
Hemoglobin hydrolysis and heme acquisition by Porphyromonas gingivalis
Dashper, S. G., Cross, K. J., Slakeski, N., Lissel, P., Aulakh, P., Moore, C., & Reynolds, E. C. (2003) · Oral Microbiology and Immunology · doi:10.1046/j.0902-0055.2003.00113.x · Cited by 39 · see citing works
Self-citationEley, B. M., & Cox, S. W. (1996) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1996.tb00507.x · Cited by 39 · see citing works
Antibacterial peptides in stimulated human granulocytes
Wang, Y., Griffiths, W. J., Jörnvall, H., Agerberth, B., & Johansson, J. (2002) · European Journal of Biochemistry · doi:10.1046/j.0014-2956.2001.02675.x · Cited by 37 · see citing works
Variability of histologic criteria in clinically healthy human gingiva
Brecx, M. C., Gautschi, M., Gehr, P., & Lang, N. P. (1987) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1987.tb02057.x · Cited by 35 · see citing works
Eley, B. M., & Cox, S. W. (1996) · Journal of Periodontology · doi:10.1902/jop.1996.67.7.703 · Cited by 33 · see citing works
Glycosaminoglycans and Periodontal Disease: Analysis of GCF by Safranin O
Giannobile, W. V., Riviere, G. R., Gorski, J. P., Tira, D. E., & Cobb, C. M. (1993) · Journal of Periodontology · doi:10.1902/jop.1993.64.3.186 · Cited by 33 · see citing works
Induction of calprotectin release by Porphyromonasgingivalis lipopolysaccharide in human neutrophils
Kido, J., Kido, R., Suryono, S., Kataoka, M., Fagerhol, M. K., & Nagata, T. (2004) · Oral Microbiology and Immunology · doi:10.1111/j.0902-0055.2004.00139.x · Cited by 33 · see citing works
Hanioka, T., Matsuse, R., Shigemoto, Y., Ojima, M., & Shizukuishi, S. (2005) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.2005.00807.x · Cited by 33 · see citing works
Hirose, K., Isogai, E., Miura, H., & 上田, 五. (1997) · Microbiology and Immunology · doi:10.1111/j.1348-0421.1997.tb01168.x · Cited by 26 · see citing works
Analysis in gingival crevicular fluid of two oligopeptides derived from human hemoglobin β‐chain
Mäkinen, K. K., Sewón, L., & Mäkinen, P. ‐. (1996) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1996.tb00462.x · Cited by 24 · see citing works
Protein recovery from several paper types used to collect gingival crevicular fluid
Johnson, R. B., Streckfus, C. F., Dai, X., & Tucci, M. A. (1999) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1999.tb02255.x · Cited by 23 · see citing works
Low molecular-weight proteins in human gingival crevicular fluid
Estreicher, A., Broggiato, A., Duroux, P., Andersen, E., & Cimasoni, G. (1996) · Archives of Oral Biology · doi:10.1016/s0003-9969(96)00076-3 · Cited by 16 · see citing works
Jepsen, S. V., Springer, I. N. G., Buschmann, A., Hedderich, J., & Açil, Y. (2003) · European Journal Of Oral Sciences · doi:10.1034/j.1600-0722.2003.00019.x · Cited by 15 · see citing works
McLaughlin, W. S., Kirkham, J., Kowolik, M. J., & Robinson, C. (1996) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1996.tb00554.x · Cited by 15 · see citing works
Analysis of Free Hemoglobin Level and Hemoglobin Peptides from Human Puerperal Uterine Secretions
Mak, P., Wicherek, Ł., Suder, P., Dubin, A., Banaś, T., Kaim, I., & Klimek, M. (2006) · Journal of the Society for Gynecologic Investigation · doi:10.1016/j.jsgi.2006.02.009 · Cited by 10 · see citing works
Salivary amylase in crevicular fluid
Smith, Q. T., & Camp, S. J. (1993) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1993.tb00382.x · Cited by 9 · see citing works
John, H., Schulz, S., & Forssmann, W. (2006) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-006-0624-x · Cited by 5 · see citing works
Soluble Fcγ receptors in periodontal lesions
Yuan, Z. ‐., Tolo, K., & Helgeland, K. (1998) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1998.tb00712.x · Cited by 3 · see citing works
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Sedghi, L., DiMassa, V., Harrington, A., Lynch, S. V., & Kapila, Y. L. (2021) · Periodontology 2000 · doi:10.1111/prd.12393 · Cited by 755 · see citing works
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Gorr, S., & Abdolhosseini, M. (2011) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2010.01664.x · Cited by 183 · see citing works
Amado, F. M. L., Calheiros‐Lobo, M. J., Domingues, P. M., Duarte, J. A., & Vitorino, R. M. P. (2010) · Expert Review of Proteomics · doi:10.1586/epr.10.48 · Cited by 119 · see citing works
Gingival crevicular fluid and its immune mediators in the proteomic era
Bostanci, N., & Belibasakis, G. N. (2018) · Periodontology 2000 · doi:10.1111/prd.12154 · Cited by 109 · see citing works
Quantitative Proteomic Analysis of Gingival Crevicular Fluid in Different Periodontal Conditions
Silva-Boghossian, C. M., Colombo, A. P. V., Tanaka, M., Rayo, C., Xiao, Y., & Siqueira, W. L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0075898 · Cited by 104 · see citing works
Salivary metabolomics in the diagnosis of oral cancer and periodontal diseases
Mikkonen, J. J. W., Singh, S. P., Herrala, M., Lappalainen, R., Myllymaa, S., & Kullaa, A. M. (2015) · Journal of Periodontal Research · doi:10.1111/jre.12327 · Cited by 99 · see citing works
Baliban, R. C., Sakellari, D., Li, Z., DiMaggio, P. A., Garcia, B. A., & Floudas, C. A. (2011) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2011.01805.x · Cited by 99 · see citing works
Grant, M. M., Creese, A. J., Barr, G., Ling, M. R., Scott, A. E., Matthews, J. B., Griffiths, H. R., Cooper, H. J., & Chapple, I. (2010) · Journal of Proteome Research · doi:10.1021/pr100446f · Cited by 86 · see citing works
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Choi, Y.-J., Heo, S.-H., Lee, J.-M., & Cho, J.-Y. (2011) · Proteome Science · doi:10.1186/1477-5956-9-42 · Cited by 71 · see citing works
Gingival crevicular fluid proteomes in health, gingivitis and chronic periodontitis
Huynh, A. H. S., Veith, P. D., McGregor, N., Adams, G. G., Chen, D., Reynolds, E. C., Ngo, L., & Darby, I. B. (2014) · Journal of Periodontal Research · doi:10.1111/jre.12244 · Cited by 69 · see citing works
Self-citationJiang, R., Rong, C., Ke, R., Meng, S., Yan, X., Ke, H., & Wu, S. (2019) · Medicine · doi:10.1097/md.0000000000017478 · Cited by 68 · see citing works
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Rody, W. J., Holliday, L. S., McHugh, K. P., Wallet, S. M., Spicer, V., & Krokhin, O. V. (2014) · American Journal of Orthodontics and Dentofacial Orthopedics · doi:10.1016/j.ajodo.2014.03.013 · Cited by 46 · see citing works
Human infant saliva peptidome is modified with age and diet transition
Morzel, M., Jeannin, A., Lucchi, G., Truntzer, C., Pecqueur, D., Nicklaus, S., Chambon, C., & Ducoroy, P. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.04.028 · Cited by 42 · see citing works
Cationic antimicrobial peptides and periodontal physiopathology: A systematic review
Jourdain, M., Velard, F., Pierrard, L., Sergheraert, J., Gangloff, S. C., & Braux, J. (2019) · Journal of Periodontal Research · doi:10.1111/jre.12676 · Cited by 41 · see citing works
Morzel, M., Palicki, O., Chabanet, C., Lucchi, G., Ducoroy, P., Chambon, C., & Nicklaus, S. (2011) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2010.12.015 · Cited by 41 · see citing works
Baliban, R. C., Sakellari, D., Li, Z., Guzman, Y. A., Garcia, B. A., & Floudas, C. A. (2012) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12037 · Cited by 40 · see citing works
Camerlingo, C., d’Apuzzo, F., Grassia, V., Perillo, L., & Lepore, M. A. (2014) · Sensors · doi:10.3390/s141222552 · Cited by 40 · see citing works
Oral fluid-based biomarkers in periodontal disease - part 2. Gingival crevicular fluid.
AlRowis, R., AlMoharib, H. S., AlMubarak, A. M., Bhaskardoss, J., Preethanath, R. S., & Anil, S. (2014) · PubMed Central · Cited by 39 · see citing works
Gingimaps: Protein Localization in the Oral Pathogen Porphyromonas gingivalis
Gabarrini, G., Grasso, S., Winkelhoff, A. J. V., & van Dijl, J. M. (2020) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00032-19 · Cited by 39 · see citing works
Salivary concentration of free LL-37 in edentulism, chronic periodontitis and healthy periodontium
Davidopoulou, S., Diza, E., Sakellari, D., Menexes, G. C., & Kalfas, S. (2013) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2013.01.003 · Cited by 35 · see citing works
Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases
Preianò, M., Savino, R., Villella, C., Pelaia, C., & Terracciano, R. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21155270 · Cited by 34 · see citing works
Monitoring early phases of orthodontic treatment by means of Raman spectroscopies
d’Apuzzo, F., Perillo, L., Delfino, I., Portaccio, M., Lepore, M. A., & Camerlingo, C. (2017) · Journal of Biomedical Optics · doi:10.1117/1.jbo.22.11.115001 · Cited by 32 · see citing works
Ngo, L., Darby, I. B., Veith, P. D., Locke, A. G., & Reynolds, E. C. (2012) · Journal of Periodontal Research · doi:10.1111/jre.12012 · Cited by 32 · see citing works
Self-citationWhat do ’omic technologies have to offer periodontal clinical practice in the future?
Grant, M. M. (2011) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.2011.01387.x · Cited by 31 · see citing works
Proteomics approach for biomarkers and diagnosis of periodontitis: systematic review
Rizal, M. I., Soeroso, Y., Sulijaya, B., Assiddiq, B. F., Bachtiar, E. W., & Bachtiar, B. M. (2020) · Heliyon · doi:10.1016/j.heliyon.2020.e04022 · Cited by 30 · see citing works
Proteomics for the discovery of biomarkers and diagnosis of periodontitis: a critical review
Guzman, Y. A., Sakellari, D., Arsenakis, M., & Floudas, C. A. (2013) · Expert Review of Proteomics · doi:10.1586/14789450.2014.864953 · Cited by 30 · see citing works
Guzman, Y. A., Sakellari, D., Papadimitriou, K., & Floudas, C. A. (2018) · Journal of Periodontal Research · doi:10.1111/jre.12575 · Cited by 29 · see citing works
Ahmad, P., Escalante‐Herrera, A., Marin, L. M., & Siqueira, W. L. (2024) · Journal of Periodontal Research · doi:10.1111/jre.13313 · Cited by 29 · see citing works
Rapid diagnosis of periodontitis, a feasibility study using MALDI-TOF mass spectrometry
Antezack, A., Chaudet, H., Tissot‐Dupont, H., Brouqui, P., & Monnet‐Corti, V. (2020) · PLoS ONE · doi:10.1371/journal.pone.0230334 · Cited by 28 · see citing works
Salivaomics - A promising future in early diagnosis of dental diseases.
Koneru, S., & Tanikonda, R. (2014) · Europe PMC (PubMed Central) · doi:10.4103/1735-3327.127178 · Cited by 28 · see citing works
Preianò, M., Maggisano, G., Lombardo, N., Montalcini, T., Paduano, S., Pelaia, G., Savino, R., & Terracciano, R. (2015) · PROTEOMICS · doi:10.1002/pmic.201500328 · Cited by 26 · see citing works
The prominent proteins expressed in healthy gingiva: a pilot exploratory tissue proteomics study
Yaprak, E., Kasap, M., Akpınar, G., Kayaaltı-Yüksek, S., Sinanoğlu, A., Güzel, N., & Kocasaraç, H. D. (2017) · Odontology · doi:10.1007/s10266-017-0302-9 · Cited by 21 · see citing works
Marinho, M. C., Pacheco, A. B. F., Costa, G., Ortiz, N. D., Zajdenverg, L., & Sansone, C. (2018) · Oral Diseases · doi:10.1111/odi.12996 · Cited by 21 · see citing works
Tsuchida, S. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21155184 · Cited by 21 · see citing works
Preianò, M., Falcone, D., Maggisano, G., Montalcini, T., Navarra, M., Paduano, S., Savino, R., & Terracciano, R. (2014) · Clinica Chimica Acta · doi:10.1016/j.cca.2014.07.022 · Cited by 21 · see citing works
Quantitative proteomic analysis of gingival crevicular fluids from deciduous and permanent teeth
Moriya, Y., Obama, T., Aiuchi, T., Sugiyama, T., Endo, Y., Koide, Y., Noguchi, E., Ishizuka, M., Inoue, M., Itabe, H., et al. (2017) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12696 · Cited by 20 · see citing works
Tong, P., Yuan, C., Sun, X., Qi, Y., Wang, X., & Zheng, S. (2018) · Clinica Chimica Acta · doi:10.1016/j.cca.2018.12.003 · Cited by 20 · see citing works
Liu, L. Y., McGregor, N., Wong, B. K. J., Butt, H., & Darby, I. B. (2015) · Journal of Periodontal Research · doi:10.1111/jre.12286 · Cited by 18 · see citing works
Self-citationKajiura, Y., Bando, M., Inagaki, Y., Nagata, T., & Kido, J. (2014) · Journal of Periodontology · doi:10.1902/jop.2014.140241 · Cited by 17 · see citing works
Antimicrobial peptide gene expression in periodontitis patients: A pilot study
Jourdain, M., Pierrard, L., Kanagaratnam, L., Velard, F., Sergheraert, J., Lefèvre, B., Gangloff, S. C., & Braux, J. (2018) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12879 · Cited by 16 · see citing works
Preianò, M., Maggisano, G., Murfuni, M. S., Villella, C., Pelaia, C., Montalcini, T., Lombardo, N., Pelaia, G., Savino, R., & Terracciano, R. (2018) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201800005 · Cited by 16 · see citing works
Preianò, M., Maggisano, G., Murfuni, M. S., Villella, C., Colica, C., Fregola, A., Pelaia, C., Lombardo, N., Pelaia, G., Savino, R., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19124005 · Cited by 15 · see citing works
Hartenbach, F. A. R. R., Silva‐Boghossian, C. M., & Colombo, A. P. V. (2017) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2017.10.007 · Cited by 14 · see citing works
On the characterisation of the porcine gland-specific salivary proteome
Prims, S., Raemdonck, G. V., Hole, C. V., Cruchten, S. J. M. V., Ginneken, C. J. V., Ostade, X. V., & Casteleyn, C. R. (2019) · Journal of Proteomics · doi:10.1016/j.jprot.2019.01.016 · Cited by 12 · see citing works
Camerlingo, C., Portaccio, M., d’Apuzzo, F., Nucci, L., Perillo, L., & Lepore, M. A. (2022) · Sensors · doi:10.3390/s22207874 · Cited by 12 · see citing works
Xiao, X., Song, T., Xiao, X., Liu, Y., Sun, H., Guo, Z., Liu, X., Shao, C., Li, Q., & Sun, W. (2021) · PROTEOMICS · doi:10.1002/pmic.202000321 · Cited by 11 · see citing works
Porphyromonas gingivalis Gingipains Display Transpeptidation Activity
Zhang, L., Veith, P. D., Huq, N. L., Chen, Y., Seers, C. A., Cross, K. J., Gorasia, D. G., & Reynolds, E. C. (2018) · Journal of Proteome Research · doi:10.1021/acs.jproteome.8b00286 · Cited by 11 · see citing works
Self-citationToczewska, J., Baczyńska, D., Zalewska, A., Maciejczyk, M., & Konopka, T. P. (2023) · Dental and Medical Problems · doi:10.17219/dmp/150888 · Cited by 10 · see citing works
Tsuchida, S., Satoh, M., Sogawa, K., Kawashima, Y., Kado, S., Ishige, T., Beppu, M., Sawai, S., Nishimura, M., Kodera, Y., et al. (2014) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201300122 · Cited by 10 · see citing works
Liu, W., Qiu, W., Huang, Z.-D., Zhang, K., Wu, K., Deng, K., Chen, Y., Guo, R., Wu, B., Chen, T., et al. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.963123 · Cited by 9 · see citing works
Self-citationApplications of Mass Spectrometry in Dentistry
Kallianta, M., Pappa, E., Vastardis, H., & Rahiotis, C. (2023) · Biomedicines · doi:10.3390/biomedicines11020286 · Cited by 7 · see citing works
Bellei, E., Monari, E., Bertoldi, C., & Bergamini, S. (2024) · Sci · doi:10.3390/sci6040057 · Cited by 6 · see citing works
Rapid Acquisition of Gingival Crevicular Fluid FT-IR Spectra for Monitoring Orthodontic Treatments
Lepore, M. A., Portaccio, M., d’Apuzzo, F., Nucci, L., Grassia, V., Annunziata, M., & Camerlingo, C. (2024) · IEEE Sensors Applications Symposium (SAS) · doi:10.1109/sas60918.2024.10636387 · Cited by 5 · see citing works
Salih, E. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6685-1_3 · Cited by 5 · see citing works
Microbes in human oral cavity: a review
Sarmah, R., Khan, R. A., & Devi, K. R. (2020) · Reviews in Medical Microbiology · doi:10.1097/mrm.0000000000000227 · Cited by 5 · see citing works
Kim, J.-P., Yun, H., Kim, E.-J., Kim, Y., Lee, C., Ko, B. J., Kim, B., & Jeong, H. (2023) · Biotechnology Letters · doi:10.1007/s10529-023-03364-0 · Cited by 4 · see citing works
Xi, W., Gu, Y., & Chen, F. (2016) · Diagnostics · doi:10.3390/diagnostics6040041 · Cited by 4 · see citing works
Proteomics of Periodontal Pocket
Sakellari, D. (2017) · Current Oral Health Reports · doi:10.1007/s40496-017-0160-0 · Cited by 3 · see citing works
Lepore, M. A., Portaccio, M., d’Apuzzo, F., Nucci, L., Grassia, V., Annunziata, M., & Camerlingo, C. (2025) · IEEE Transactions on Instrumentation and Measurement · doi:10.1109/tim.2025.3565240 · Cited by 2 · see citing works
Pietro, L. D., Boroumand, M., Lattanzi, W., Manconi, B., Salvati, M., Cabras, T., Olianas, A., Flore, L., Serrao, S., Calò, C. M., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241915010 · Cited by 2 · see citing works
Yang, X., Wang, J., Liao, R., & Yan, C. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342342 · Cited by 2 · see citing works
Biomarkers in Gingival Diseases: Current Insights and Future Perspectives
George, A. K., Malaiappan, S., Joseph, B., & Anil, S. (2024) · Dentistry · doi:10.5772/intechopen.114267 · Cited by 2 · see citing works
Omics’ in oral cancer: A comprehensive review
Sachdev, S. S., Sardar, M. A., Tupkari, J. V., Chettiankandy, T. J., Dalvi, S. M., & D’souza, Z. I. (2022) · International Journal of Orofacial Research · doi:10.56501/intjorofacres.v6i2.152 · Cited by 1 · see citing works
Proteomic Analysis of Gingival Crevicular Fluid During Tooth Eruption
ALBAT, S. Y., Akyüz, S., Yarat, A., Şahi̇n, F., & Çimen, H. (2023) · Clinical and Experimental Health Sciences · doi:10.33808/clinexphealthsci.1214974 · Cited by 1 · see citing works
The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study
Badovinac, A., Razdorov, G., Grgurević, L., Puhar, I., Plančak, D., & Božić, D. (2013) · Acta Stomatologica Croatica · doi:10.15644/asc47/1/2 · Cited by 1 · see citing works
Ecological Therapeutic Opportunities for Oral Diseases
Hoare, A., Marsh, P. D., & Diaz, P. I. (2018) · ASM Press eBooks · doi:10.1128/9781555819705.ch10
Current literature in mass spectrometry
(2010) · Journal of Mass Spectrometry · doi:10.1002/jms.1659
Zhou, F., Wu, Y., Peng, X., Li, Y., Nie, W., Wang, X., Ren, B., & Li, J. (2026) · Nature Communications · doi:10.1038/s41467-026-77383-6
- Authors
- Ngo, L. H., Veith, P. D., Chen, Y.-Y., Chen, D., Darby, I. B., & Reynolds, E. C.
- Affiliations
- The University of Melbourne (AU)
- Corresponding author
- Reynolds, Eric C.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-01-22
- Volume
- 9
- Issue
- 4
- Pages
- 1683-1693
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2010350235
- Cited by
- 82
- References
- 75
- Citations by year
- 2025: 1 · 2024: 7 · 2023: 5 · 2022: 4 · 2021: 2 · 2020: 8 · 2019: 5 · 2018: 8 · 2017: 7 · 2016: 2 · 2015: 3 · 2014: 8 · 2013: 5 · 2012: 5
- FWCI
- 5.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- gingival crevicular fluid, periodontal disease, periodontitis, proteome, peptide identification, actin, actin-binding proteins, profilin, cofilin, gelsolin, periodontal inflammation
- MeSH terms
- Adult, Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Gingival Crevicular Fluid, Humans, Molecular Sequence Data, Peptide Fragments, Periodontal Diseases, Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, alpha-Defensins, Tandem Mass Spectrometry
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