Cited by 83Oral microbiology and periodontitis researchAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and Applications

Mass Spectrometric Analyses of Peptides and Proteins in Human Gingival Crevicular Fluid

Ngo, Luan H., Veith, Paul D., Chen, Yu-Yen, Chen, Dina, Darby, Ivan B., Reynolds, Eric C.·2010·Journal of Proteome Research

Abstract

Gingival crevicular fluid (GCF) is a pathophysiological fluid that flows into the oral cavity. Human GCF was collected using sterile glass microcapillary tubes from inflamed periodontal sites in patients who had a history of periodontal disease and were in the maintenance phase of treatment. Samples from individual sites were analyzed using MS techniques both before and following HPLC. GCF samples were also pooled and subjected to SDS-PAGE, in-gel digestion and MS analyses using both MALDI-TOF/TOF MS and nanoLC-ESI-MS/MS. MS spectra were used to search human protein sequence databases for protein identification. With these approaches, 33 peptides and 66 proteins were positively identified in human GCF. All of the peptides discovered in this study are reported in GCF here for the first time. Forty-three of the identified proteins, such as actin and the actin binding proteins profilin, cofilin and gelsolin, have not been reported in GCF before.

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Crossref · 10.1021/pr900775s

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Authors
Ngo, L. H., Veith, P. D., Chen, Y.-Y., Chen, D., Darby, I. B., & Reynolds, E. C.
Affiliations
The University of Melbourne (AU)
Corresponding author
Reynolds, Eric C.
Venue
Journal of Proteome Research
Published
2010-01-22
Volume
9
Issue
4
Pages
1683-1693
ISSN
  • 1535-3893 (ISSN-L)
  • 1535-3907
OpenAlex
W2010350235
Cited by
82
References
75
Citations by year
2025: 1 · 2024: 7 · 2023: 5 · 2022: 4 · 2021: 2 · 2020: 8 · 2019: 5 · 2018: 8 · 2017: 7 · 2016: 2 · 2015: 3 · 2014: 8 · 2013: 5 · 2012: 5
FWCI
5.61
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteome Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Keywords
gingival crevicular fluid, periodontal disease, periodontitis, proteome, peptide identification, actin, actin-binding proteins, profilin, cofilin, gelsolin, periodontal inflammation
MeSH terms
Adult, Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Gingival Crevicular Fluid, Humans, Molecular Sequence Data, Peptide Fragments, Periodontal Diseases, Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, alpha-Defensins, Tandem Mass Spectrometry

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