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Elevated Levels of G-Quadruplex Formation in Human Stomach and Liver Cancer Tissues
Open accessAbstract
Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.
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“Additionally, G-rich sequences, like G4 motifs, are prone to oxidative DNA damage, which can lead to mutations if not repaired properly (76).”
From G-quadruplex-mediated genomic instability drives SNVs in cancer (2024) · cites this paper as [76]
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“This suggests that elevated G-quadruplex formation might be a characteristic of some cancers and supports the hypothesis that G4s could contribute to genomic instability in cancer patients (76).”
From G-quadruplex-mediated genomic instability drives SNVs in cancer (2024) · cites this paper as [76]
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“recent studies have shown that G4s can be detected in patient-derived tissues using immunohistochemistry, with a significantly elevated number of G4-positive nuclei observed in human cancers of the liver and stomach compared to non-neoplastic tissue (76).”
From G-quadruplex-mediated genomic instability drives SNVs in cancer (2024) · cites this paper as [76]
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Background
Cited by
Sorted by citations
High-throughput sequencing of DNA G-quadruplex structures in the human genome
Chambers, V. S., Marsico, G., Boutell, J. M., Antonio, M. D., Smith, G. P., & Balasubramanian, S. (2015) · Nature Biotechnology · doi:10.1038/nbt.3295 · Cited by 1,261
Self-citationThe regulation and functions of DNA and RNA G-quadruplexes
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Self-citationG-quadruplex structures mark human regulatory chromatin
Hänsel-Hertsch, R., Beraldi, D., Lensing, S. V., Marsico, G., Zyner, K., Parry, A., Di Antonio, M., Pike, J., Kimura, H., Narita, M., et al. (2016) · Nature Genetics · doi:10.1038/ng.3662 · Cited by 964
Self-citationThe Structure and Function of DNA G-Quadruplexes
Spiegel, J., Adhikari, S., & Balasubramanian, S. (2020) · Trends in Chemistry · doi:10.1016/j.trechm.2019.07.002 · Cited by 920
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Hänsel‐Hertsch, R., Antonio, M. D., & Balasubramanian, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.3 · Cited by 882
Self-citation
Metadata
- Authors
- Biffi, G., Tannahill, D., Miller, J., Howat, W. J., & Balasubramanian, S.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Balasubramanian, Shankar
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-07-17
- Volume
- 9
- Issue
- 7
- Pages
- e102711
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4102534
- OpenAlex
- W2100966371
- Cited by
- 223
- References
- 34
- Citations by year
- 2026: 8 · 2025: 17 · 2024: 27 · 2023: 31 · 2022: 20 · 2021: 30 · 2020: 25 · 2019: 15 · 2018: 14 · 2017: 17 · 2016: 7 · 2015: 10 · 2014: 2
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Figshare (submittedVersion, cc-by)
- Funders
- Cancer Research UK (11961, A11961, 12488, 22310, C9681/A11961)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Keywords
- G-quadruplex, G-quadruplex formation, immunohistochemistry, gastric cancer, liver cancer, tumor markers, DNA secondary structure, patient-derived tissue
- MeSH terms
- DNA, Gastric Mucosa, Humans, Immunohistochemistry, Liver, Liver Neoplasms, Stomach Neoplasms, Cell Line, Tumor, G-Quadruplexes
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