The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System
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Due to the high efficiency of Cas9 activity and the relatively higher efficiency of NHEJ when compared to HDR, three kinds of entities coexist in this process: wild-type sequences, NHEJ-repaired sequences, and a smaller population of the intended HDR-repaired sequence.
“Due to the high efficiency of Cas9 activity and the relatively higher efficiency of NHEJ when compared to HDR, three kinds of entities coexist in this process: wild-type sequences, NHEJ-repaired sequences, and a smaller population of the intended HDR-repaired sequence [73].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [73]
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Cites
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.04.025 · Cited by 3,495
Mali, P., Aach, J., Stranges, P. B., Esvelt, K. M., Moosburner, M., Kosuri, S., Yang, L., & Church, G. M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2675 · Cited by 1,752 · cited 1×
“It has been suggested that a ‘‘dual nick’’ strategy, in which two gRNAs direct the Cas9D10A single strand nickase activity to nearby sites to yield an effective double strand break, will be a effective means to minimize off-target mutagenesis [11,13], because the two gRNAs will rarely yield nearby nicks outside of the…”
BackgroundDe novo mutations in histone-modifying genes in congenital heart disease
Zaidi, S., Choi, M., Wakimoto, H., Ma, L., Jiang, J., Overton, J. D., Romano-Adesman, A., Bjornson, R. D., Breitbart, R. E., Brown, K. K., et al. (2013) · Nature · doi:10.1038/nature12141 · Cited by 934
Flagged first, then by citations
Research works printed but not linked: 14
- D Hockemeyer, 2011, Genetic engineering of human pluripotent cells using TALE nucleases, Nat Biotechnol, 29, 731, 10.1038/nbt.1927 · DOI
- T Cermak, 2011, Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting, Nucleic Acids Res, 39, e82, 10.1093/nar/gkr218 · DOI
- L Cong, 2013, Multiplex genome engineering using CRISPR/Cas systems, Science, 339, 819, 10.1126/science.1231143 · DOI · Paper page
- Mali P, Yang L, Esvelt KM, Aach J, Guell M, <etal>et al</etal>.. (2013) RNA-Guided Human Genome Engineering via Cas9. Science. · DOI · Paper page
- L Yang, 2013, Optimization of scarless human stem cell genome editing, Nucleic Acids Res, 41, 9049, 10.1093/nar/gkt555 · DOI
- Y Miyaoka, 2014, Isolation of single-base genome-edited human iPS cells without antibiotic selection, Nat Methods, 11, 291, 10.1038/nmeth.2840 · DOI
- P Liu, 2003, A highly efficient recombineering-based method for generating conditional knockout mutations, Genome Res, 13, 476, 10.1101/gr.749203 · DOI
- DG Gibson, 2009, Enzymatic assembly of DNA molecules up to several hundred kilobases, Nat Methods, 6, 343, 10.1038/nmeth.1318 · DOI
- P Zhou, 2013, Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification, Proc Natl Acad Sci U S A, 110, 15395, 10.1073/pnas.1304124110 · DOI
- FA Ran, 2013, Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity, Cell, 154, 1380, 10.1016/j.cell.2013.08.021 · DOI · Paper page
- Shen B, Zhang W, Zhang J, Zhou J, Wang J, <etal>et al</etal>.. (2014) Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. · DOI · Paper page
- E de Boer, 2003, Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice, Proc Natl Acad Sci U S A, 100, 7480, 10.1073/pnas.1332608100 · DOI
- I Zhidkov, 2011, CHILD: a new tool for detecting low-abundance insertions and deletions in standard sequence traces, Nucleic Acids Res, 39, e47, 10.1093/nar/gkq1354 · DOI
- A Hendel, 2014, Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing, Cell Rep, 7, 293, 10.1016/j.celrep.2014.02.040 · DOI
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Carlson-Stevermer, J., Abdeen, A. A., Kohlenberg, L., Goedland, M., Molugu, K., Lou, M., & Saha, K. (2017) · Nature Communications · doi:10.1038/s41467-017-01875-9 · Cited by 170
Loureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
Current Strategies for Increasing Knock-In Efficiency in CRISPR/Cas9-Based Approaches
Leal, A., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25052456 · Cited by 58
Metadata
- Authors
- Li, K., Wang, G., Andersen, T., Zhou, P., & Pu, W. T.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-08-28
- Volume
- 9
- Issue
- 8
- Pages
- e105779
- ISSN
- 1932-6203
- PMC
- PMC4148324
- Cited by
- 123
- References
- 4
- Language
- en
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