The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat
Open accessAbstract
The development of isothermal amplification platforms for nucleic acid detection has the potential to increase access to molecular diagnostics in low resource settings; however, simple, low-cost methods for heating samples are required to perform reactions. In this study, we demonstrated that human body heat may be harnessed to incubate recombinase polymerase amplification (RPA) reactions for isothermal amplification of HIV-1 DNA. After measuring the temperature of mock reactions at 4 body locations, the axilla was chosen as the ideal site for comfortable, convenient incubation. Using commonly available materials, 3 methods for securing RPA reactions to the body were characterized. Finally, RPA reactions were incubated using body heat while control RPA reactions were incubated in a heat block. At room temperature, all reactions with 10 copies of HIV-1 DNA and 90% of reactions with 100 copies of HIV-1 DNA tested positive when incubated with body heat. In a cold room with an ambient temperature of 10 degrees Celsius, all reactions containing 10 copies or 100 copies of HIV-1 DNA tested positive when incubated with body heat. These results suggest that human body heat may provide an extremely low-cost solution for incubating RPA reactions in low resource settings.
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Cites
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
DNA Detection Using Recombination Proteins
Piepenburg, O., Williams, C. H., Stemple, D. L., & Armes, N. A. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040204 · Cited by 2,482
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Genomic Insights to Control the Emergence of Vancomycin-Resistant Enterococci
Howden, B. P., Holt, K. E., Lam, M. M. C., Seemann, T., Ballard, S., Coombs, G. W., Tong, S. Y. C., Grayson, M. L., Johnson, P. D. R., & Stinear, T. P. (2013) · mBio · doi:10.1128/mbio.00412-13 · Cited by 227
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A Lateral Flow Assay for Quantitative Detection of Amplified HIV-1 RNA
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Self-citationLillis, L., Lehman, D., Singhal, M. C., Cantera, J., Singleton, J., Labarre, P., Toyama, A., Piepenburg, O., Parker, M., Wood, R., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0108189 · Cited by 162
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Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
Pardee, K., Green, A. A., Takahashi, M. K., Braff, D., Lambert, G., Lee, J. W., Ferrante, T., Ma, D., Donghia, N., Fan, M., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.059 · Cited by 1,439
Metadata
- Authors
- Crannell, Z. A., Rohrman, B., & Richards-Kortum, R.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-11-05
- Volume
- 9
- Issue
- 11
- Pages
- e112146
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2001193282
- Cited by
- 276
- References
- 15
- FWCI
- 6.79
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
