The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
Open accessAbstract
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. PAPERCLIP.
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Citations
Cites
Zika Virus Associated with Microcephaly
Mlakar, J., Korva, M., Tul, N., Popović, M., Poljšak-Prijatelj, M., Mraz, J., Kolenc, M., Resman Rus, K., Vesnaver Vipotnik, T., Fabjan Vodušek, V., et al. (2016) · New England Journal of Medicine · doi:10.1056/nejmoa1600651 · Cited by 2,884
Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Self-citationCRISPR/Cas Systems towards Next-Generation Biosensing
Li, Y., Li, S., Wang, J., & Liu, G. (2019) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.12.005 · Cited by 917
Xiong, Y., Zhang, J., Yang, Z., Mou, Q., Ma, Y., Xiong, Y., & Lu, Y. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b09211 · Cited by 689
Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
Liu, Y., Zhan, L., Qin, Z., Sackrison, J., & Bischof, J. C. (2021) · ACS Nano · doi:10.1021/acsnano.0c10035 · Cited by 626
Design and regulation of engineered bacteria for environmental release
Chemla, Y., Sweeney, C. J., Wozniak, C. A., & Voigt, C. A. (2025) · Nature Microbiology · doi:10.1038/s41564-024-01918-0 · Cited by 142
Metadata
- Authors
- Pardee, K., Green, A. A., Takahashi, M. K., Braff, D., Lambert, G., Lee, J. W., Ferrante, T., Ma, D., Donghia, N., Fan, M., Daringer, N. M., Bosch, I., Dudley, D. M., O’Connor, D. H., Gehrke, L., & Collins, J. J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 165
- Issue
- 5
- Pages
- 1255-1266
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2346028445
- Cited by
- 1,439
- References
- 1
- FWCI
- 48.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Pardee, K., Green, A. A., Takahashi, M. K., Braff, D., Lambert, G., Lee, J. W., Ferrante, T., Ma, D., Donghia, N., Fan, M., Daringer, N. M., Bosch, I., Dudley, D. M., O’Connor, D. H., Gehrke, L., & Collins, J. J. (2016). Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. https://doi.org/10.1016/j.cell.2016.04.059
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