The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity
Open accessAbstract
Epac-based FRET sensors have been widely used for the detection of cAMP concentrations in living cells. Originally developed by us as well as others, we have since then reported several important optimizations that make these sensors favourite among many cell biologists. We here report cloning and characterization of our fourth generation of cAMP sensors, which feature outstanding photostability, dynamic range and signal-to-noise ratio. The design is based on mTurquoise2, currently the brightest and most bleaching-resistant donor, and a new acceptor cassette that consists of a tandem of two cp173Venus fluorophores. We also report variants with a single point mutation, Q270E, in the Epac moiety, which decreases the dissociation constant of cAMP from 9.5 to 4 μM, and thus increases the affinity ~ 2.5-fold. Finally, we also prepared and characterized dedicated variants with non-emitting (dark) acceptors for single-wavelength FLIM acquisition that display an exceptional near-doubling of fluorescence lifetime upon saturation of cAMP levels. We believe this generation of cAMP outperforms all other sensors and therefore recommend these sensors for all future studies.
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Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., & Miyawaki, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0400417101 · Cited by 1,030
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
Griesbeck, O., Baird, G. S., Campbell, R. E., Zacharias, D. A., & Tsien, R. Y. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102815200 · Cited by 1,023
Circular permutation and receptor insertion within green fluorescent proteins
Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.20.11241 · Cited by 961
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
Goedhart, J., von Stetten, D., Noirclerc-Savoye, M., Lelimousin, M., Joosen, L., Hink, M. A., van Weeren, L., Gadella, T. W. J., & Royant, A. (2012) · Nature Communications · doi:10.1038/ncomms1738 · Cited by 787
Self-citationNovel Single Chain cAMP Sensors for Receptor-induced Signal Propagation
Nikolaev, V. O., Bünemann, M., Hein, L., Hannawacker, A., & Lohse, M. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.c400302200 · Cited by 688
DiPilato, L. M., Cheng, X., & Zhang, J. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405973101 · Cited by 464
Ponsioen, B., Zhao, J., Riedl, J., Zwartkruis, F., van der Krogt, G., Zaccolo, M., Moolenaar, W. H., Bos, J. L., & Jalink, K. (2004) · EMBO reports · doi:10.1038/sj.embor.7400290 · Cited by 444
Self-citationBright cyan fluorescent protein variants identified by fluorescence lifetime screening
Goedhart, J., van Weeren, L., Hink, M. A., Vischer, N. O. E., Jalink, K., & Gadella, T. W. J. (2010) · Nature Methods · doi:10.1038/nmeth.1415 · Cited by 283
Self-citationMarkwardt, M. L., Kremers, G.-J., Kraft, C. A., Ray, K., Cranfill, P. J. C., Wilson, K. A., Day, R. N., Wachter, R. M., Davidson, M. W., & Rizzo, M. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017896 · Cited by 267
Ganesan, S., Ameer-beg, S. M., Ng, T. T. C., Vojnovic, B., & Wouters, F. S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0509922103 · Cited by 232
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Wang, Z., Liu, D., Varin, A., Nicolas, V, Courilleau, D., Mateo, P., Caubere, C., Rouet, P., Gomez, A.-M., Vandecasteele, G., et al. (2016) · Cell Death & Disease · doi:10.1038/cddis.2016.106 · Cited by 106
Liu, B., Mosienko, V., Vaccari Cardoso, B., Prokudina, D., Huentelman, M., Teschemacher, A. G., & Kasparov, S. (2018) · Glia · doi:10.1002/glia.23480 · Cited by 96
Glucose controls glucagon secretion by directly modulating cAMP in alpha cells
Yu, Q., Shuai, H., Ahooghalandari, P., Gylfe, E., & Tengholm, A. (2019) · Diabetologia · doi:10.1007/s00125-019-4857-6 · Cited by 84
Overcoming limitations of FRET measurements
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Metadata
- Authors
- Klarenbeek, J., Goedhart, J., van Batenburg, A., Groenewald, D., & Jalink, K.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-04-14
- Volume
- 10
- Issue
- 4
- Pages
- e0122513
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2063557162
- Cited by
- 311
- References
- 18
- FWCI
- 13.81
- Type
- journal article
- Language
- en
- Open access
- gold
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- Klarenbeek, J., Goedhart, J., van Batenburg, A., Groenewald, D., & Jalink, K. (2015). Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity. PLOS ONE. https://doi.org/10.1371/journal.pone.0122513
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