Head and neck veins of the mouse. A magnetic resonance, micro computed tomography and high frequency color Doppler ultrasound study
Open accessAbstract
To characterize the anatomy of the venous outflow of the mouse brain using different imaging techniques. Ten C57/black male mice (age range: 7-8 weeks) were imaged with high-frequency Ultrasound, Magnetic Resonance Angiography and ex-vivo Microcomputed tomography of the head and neck. Under general anesthesia, Ultrasound of neck veins was performed with a 20 MHz transducer; head and neck Magnetic Resonance Angiography data were collected on 9.4 T or 7 T scanners, and ex-vivo Microcomputed tomography angiography was obtained by filling the vessels with a radiopaque inert silicone rubber compound. All procedures were approved by the local ethical committee. The dorsal intracranial venous system is quite similar in mice and humans. Instead, the mouse Internal Jugular Veins are tiny vessels receiving the sigmoid sinuses and tributaries from cerebellum, occipital lobe and midbrain, while the majority of the cerebral blood, i.e. from the olfactory bulbs and fronto-parietal lobes, is apparently drained through skull base connections into the External Jugular Vein. Three main intra-extracranial anastomoses, absent in humans, are: 1) the petrosquamous sinus, draining into the posterior facial vein, 2) the veins of the olfactory bulb, draining into the superficial temporal vein through a foramen of the frontal bone 3) the cavernous sinus, draining in the External Jugular Vein through a foramen of the sphenoid bone. The anatomical structure of the mouse cranial venous outflow as depicted by Ultrasound, Microcomputed tomography and Magnetic Resonance Angiography is different from humans, with multiple connections between intra- and extra-cranial veins.
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While no overt regionalization of endothelial cell programs was observed, venous markers were enriched in the 3V ChP across all ages.
“While we did not observe overt regionalization of endothelial cell programs, venous markers were enriched in the 3V ChP across all ages (Figure 6D, S6F,G), likely resulting from capture of the proximal vein of Galen (Mancini et al., 2015) (Figure S6H).”
From A cellular and spatial map of the choroid plexus across brain ventricles and ages (2021) · cites this paper as Mancini et al., 2015
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- Authors
- Mancini, M., Greco, A., Tedeschi, E., Palma, G., Ragucci, M., Bruzzone, M. G., Coda, A. R. D., Torino, E., Scotti, A., Zucca, I., & Salvatore, M.
- Affiliations
- Institute of Biostructure and Bioimaging (IT)
- CEINGE Biotecnologie Avanzate Franco Salvatore (Italy) (IT)
- University of Naples Federico II (IT)
- Fondazione IRCCS Istituto Neurologico Carlo Besta (IT)
- Italian Institute of Technology (IT)
- SDN Istituto di Ricerca Diagnostica e Nucleare (IT)
- Corresponding author
- Mancini, Marcello
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-06-11
- Volume
- 10
- Issue
- 6
- Pages
- e0129912
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4466257
- OpenAlex
- W1409809438
- Cited by
- 36
- References
- 25
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 7 · 2023: 5 · 2022: 1 · 2021: 6 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 1
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Neurology › Cerebral Venous Sinus Thrombosis
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Anatomy and Medical Technology
- Keywords
- internal jugular vein, external jugular vein, cavernous sinus, magnetic resonance angiography, high-frequency color Doppler ultrasound
- MeSH terms
- Animals, Cerebral Veins, Head, Jugular Veins, Male, Mice, Inbred C57BL, Neck, Ultrasonics, Echocardiography, Doppler, Color, Magnetic Resonance Angiography, Mice, X-Ray Microtomography
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