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Cited by 44Heavy Metal Exposure and ToxicityHeavy metals in environmentEnvironmental Toxicology and Ecotoxicology

Water spinach, Ipomoea aquatica (Convolvulaceae), ameliorates lead toxicity by inhibiting oxidative stress and apoptosis

Open access
Dewanjee, Saikat, Dua, Tarun K., Khanra, Ritu, Das, Shilpa, Barma, Sujata, Joardar, Swarnalata, Bhattacharjee, Niloy, Zia-Ul-Haq, M. +1 more·2015·PLOS ONE
DOI 10.1371/journal.pone.0139831PMC PMC4608788
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Abstract

BACKGROUND: Ipomoea aquatica (Convolvulaceae), an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous) extract of I. aquatica (AEIA) against experimentally induced Pb-intoxication. METHODS: The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM) along with AEIA (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg) against Pb-acetate (5 mg/kg) intoxication. RESULTS: Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01) the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored significantly the evaluated-parameters to the near-normal position. CONCLUSION: The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating. The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

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Crossref · 10.1371/journal.pone.0139831

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  • Lead poisoning causes oxidative stress mediated by ROS, which causes protein oxidation, lipid and nucleic acid peroxidation, and eventually death.

    “Lead poisoning causes oxidative stress-mediated by ROS, which causes protein oxidation, lipid, nucleic acid peroxidation, and eventually death (Nagajyoti et al., 2010; Dewanjee et al., 2015; Li et al., 2016).”

    From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Dewanjee et al., 2015

    The Discussion (page unavailable) states that lead-induced ROS damage lipids, proteins and nucleic acids, and describes lipid peroxidation and protein carbonylation. It also links ROS to apoptosis in lead-exposed cells. The paper does not establish nucleic-acid peroxidation specifically or death of the organism; its death-related finding is cellular apoptosis. (LLM, conf=0.85)

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Research works printed but not linked: 2

  1. [5]Bressler J, Kim KA, Chakraborti T, Goldstein G. Mechanism of lead neurotoxicity. Neurochem Res. 1999; 24: 595– 600. 10227691
  2. [28]Rangan GK, Tesch GH. Quantification of renal pathology by image analysis. Nephrol. 2007; 12: 553– 8. 17995580

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Authors
Dewanjee, S., Dua, T. K., Khanra, R., Das, S., Barma, S., Joardar, S., Bhattacharjee, N., Zia-Ul-Haq, M., & Jaafar, H. Z. E.
Affiliations
  • Jadavpur University (IN)
  • Universiti Putra Malaysia (MY)
Corresponding author
  • Dewanjee, Saikat
  • Jaafar, Hawa Z. E.
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2015-10-16
Volume
10
Issue
10
Pages
e0139831
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0139831
PMC
PMC4608788
OpenAlex
W2210431284
Cited by
44
References
39
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 4 · 2015: 1
FWCI
3.13
Type
journal article
Language
en
Update notices
correction (2015-11-24)
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Greater South Information System
Funders
Jadavpur University
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
  • Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
Keywords
water spinach, lead toxicity, lead acetate, oxidative stress, apoptosis, hepatoprotection, antioxidant enzymes, lipid peroxidation, protein carbonylation, glutathione, caspases, Bcl-2 family proteins, lead bioaccumulation, heavy metal detoxification, medicinal plants, hepatocytes, mice, serum biochemistry
MeSH terms
Animals, Cells, Cultured, Lead Poisoning, Organometallic Compounds, Plant Extracts, Apoptosis, Oxidative Stress, Cytoprotection, Hepatocytes, Ipomoea, Mice

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