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Lactic Acid Bacteria Isolated from Bovine Mammary Microbiota: Potential Allies against Bovine Mastitis
Open accessAbstract
Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
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“In this case, L. helveticus 34.9 can be considered to have a medium hydrophobic character.”
From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [33]
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Cites
R: A Language and Environment for Statistical Computing
R Core Team (2000) · doi:10.32614/r.manuals · Cited by 355,893
CDD: NCBI's conserved domain database
Marchler‐Bauer, A., Derbyshire, M. K., Gonzales, N. R., Lu, S., Chitsaz, F., Geer, L. Y., Geer, R. C., He, J., Gwadz, M., Hurwitz, D. I., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1221 · Cited by 3,468
Identifying bacterial genes and endosymbiont DNA with Glimmer
Delcher, A. L., Bratke, K. A., Powers, E. C., & Salzberg, S. L. (2007) · Bioinformatics · doi:10.1093/bioinformatics/btm009 · Cited by 3,157
Host interactions of probiotic bacterial surface molecules: comparison with commensals and pathogens
Lebeer, S., Vanderleyden, J., & De Keersmaecker, S. C. J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2297 · Cited by 1,062
Godon, J., Zumstein, E., Dabert, P., Habouzit, F., & Moletta, R. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.7.2802-2813.1997 · Cited by 924
Arroyo, R., Martín, V., Maldonado‐Barragán, A., Jiménez, E., Fernández, L., & Rodríguez, J. M. (2010) · Clinical Infectious Diseases · doi:10.1086/652763 · Cited by 365
[28] Purification, specific fragmentation, and separation of large DNA molecules
Smith, C. L., & Cantor, C. R. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)55030-3 · Cited by 348
Probiotics: Properties, Examples, and Specific ApplicationsPreprint
Behnsen, J., Deriu, E., Sassone‐Corsi, M., & Raffatellu, M. (2013) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a010074 · Cited by 305
Sengupta, R., Altermann, E., Anderson, R. C., McNabb, W. C., Moughan, P. J., & Roy, N. C. (2013) · Mediators of Inflammation · doi:10.1155/2013/237921 · Cited by 301
Mastitis: Comparative Etiology and Epidemiology
Contreras, G. A., & Rodríguez, J. M. (2011) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-011-9234-0 · Cited by 286
Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes
Bierne, H., Sabet, C., Personnic, N., & Cossart, P. (2007) · Microbes and Infection · doi:10.1016/j.micinf.2007.05.003 · Cited by 277
Riollet, C., Rainard, P., & Poutrel, B. (2000) · Clinical and Diagnostic Laboratory Immunology · doi:10.1128/cdli.7.2.161-167.2000 · Cited by 245
Molecular Correlates of Host Specialization in Staphylococcus aureus
Herron‐Olson, L., Fitzgerald, J. R., Musser, J. M., & Kapur, V. (2007) · PLoS ONE · doi:10.1371/journal.pone.0001120 · Cited by 245
Charlier, C., Cretenet, M., Even, S., & Le Loir, Y. (2009) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2008.06.032 · Cited by 215
Self-citationMastitis impact on technological properties of milk and quality of milk products—a review
Maréchal, C. L., Thiéry, R., Vautor, E., & Loir, Y. L. (2011) · Dairy Science and Technology · doi:10.1007/s13594-011-0009-6 · Cited by 197
Treatment for bovine E scherichia colimastitis – an evidence‐based approach
Suojala, L., Kaartinen, L., & Pyörälä, S. (2013) · Journal of Veterinary Pharmacology and Therapeutics · doi:10.1111/jvp.12057 · Cited by 171
Adherent and invasive Escherichia coli are associated with persistent bovine mastitis
Dogan, B., Klaessig, S., Rishniw, M., Almeida, R. A., Oliver, S. P., Simpson, K. W., & Schukken, Y. H. (2006) · Veterinary Microbiology · doi:10.1016/j.vetmic.2006.04.023 · Cited by 162
Complete Genome Sequence of the Probiotic Lactobacillus casei Strain BL23
Mazé, A., Boël, G., Zúñiga, M., Bourand-Plantefol, A., Loux, V., Yebra, M. J., Monedero, V., Correia, K., Jacques, N., Beaufils, S., et al. (2010) · Journal of Bacteriology · doi:10.1128/jb.00076-10 · Cited by 149
Tomás, M. S. J., Ocaña, V. S., Wiese, B., & Nader-Macı́as, M. E. (2003) · Journal of Medical Microbiology · doi:10.1099/jmm.0.05155-0 · Cited by 138
Barinov, A., Loux, V., Hammani, A., Nicolas, P., Langella, P., Ehrlich, S. D., Maguin, E., & van de Guchte, M. (2008) · PROTEOMICS · doi:10.1002/pmic.200800195 · Cited by 136
Self-citationBouchard, D. S., Rault, L., Berkova, N., Loir, Y. L., & Even, S. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.03323-12 · Cited by 124
Self-citationLactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis
Espeche, M. C., Pellegrino, M., Frola, I. D., Larriestra, A. J., Bogni, C. I., & Nader-Macı́as, M. E. (2012) · Anaerobe · doi:10.1016/j.anaerobe.2012.01.002 · Cited by 119
Lactobacillaceae and Cell Adhesion: Genomic and Functional Screening
Turpin, W., Humblot, C., Noordine, M.-L., Thomas, M., & Guyot, J.-P. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038034 · Cited by 118
Klostermann, K., Crispie, F., Flynn, J., Ross, R. P., Hill, C., & Meaney, W. J. (2008) · Journal of Dairy Research · doi:10.1017/s0022029908003373 · Cited by 114
Kechaou, N., Chain, F., Gratadoux, J., Blugeon, S., Bertho, N., Chevalier, C., Goffic, R. L., Courau, S., Molimard, P., Chatel, J., et al. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.03075-12 · Cited by 113
Self-citationAdhesion Forces and Coaggregation between Vaginal Staphylococci and Lactobacilli
Younes, J. A., van der Mei, H. C., van den Heuvel, E. R., Busscher, H. J., & Reid, G. (2012) · PLoS ONE · doi:10.1371/journal.pone.0036917 · Cited by 106
Innocentin, S., Guimarães, V., Miyoshi, A., Azevedo, V., Langella, P., Chatel, J., & Lefèvre, F. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.00825-09 · Cited by 105
Self-citationEspeche, M. C., Otero, M. C., Sesma, F. J. M., & Nader-Macı́as, M. E. (2008) · Veterinary Microbiology · doi:10.1016/j.vetmic.2008.09.078 · Cited by 96
Probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle
Otero, M. C., Morelli, L., & Nader-Macı́as, M. E. (2006) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2006.01914.x · Cited by 89
Roussel, P., Cunha, P., Porcherie, A., Petzl, W., Gilbert, F. B., Riollet, C., Zerbe, H., Rainard, P., & Germon, P. (2015) · Veterinary Research · doi:10.1186/s13567-015-0201-4 · Cited by 88
Self-citationSelecting, testing and understanding probiotic microorganisms
Reid, G., Kim, S. O., & Koehler, G. (2005) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2005.00026.x · Cited by 82
Advances in the design of probiotic products for the prevention of major diseases in dairy cattle
Nader-Macı́as, M. E., Otero, M. C., Espeche, M. C., & Maldonado, N. C. (2008) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0438-2 · Cited by 76
Prevalence of coaggregation reactions among chicken lactobacilli
Vandevoorde, L., Christiaens, H., & Verstraete, W. (1992) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1992.tb01826.x · Cited by 72
Suojala, L., Simojoki, H., Mustonen, K., Kaartinen, L., & Pyörälä, S. (2010) · Journal of Dairy Science · doi:10.3168/jds.2009-2462 · Cited by 68
Effects of intramammary inoculation ofLactobacillus perolensCRL1724 in lactating cows’ udders
Frola, I. D., Pellegrino, M., Espeche, M. C., Giraudo, J., Nader-Macı́as, M. E., & Bogni, C. I. (2011) · Journal of Dairy Research · doi:10.1017/s0022029911000835 · Cited by 67
Lortal, S., Rouault, A., Guezenec, S. X. E. P., & Gautier, M. (1997) · Current Microbiology · doi:10.1007/s002849900165 · Cited by 47
Phenol‐soluble modulin α induces G2/M phase transition delay in eukaryotic HeLa cells
Deplanche, M., Filho, R. A. E.-A., Alekseeva, L. G., Ladier, E., Jardin, J., Henry, G., Azevedo, V., Miyoshi, A., Beraud, L., Laurent, F. J., et al. (2015) · The FASEB Journal · doi:10.1096/fj.14-260513 · Cited by 31
Genome Sequence of Staphylococcus aureus Newbould 305, a Strain Associated with Mild Bovine Mastitis
Bouchard, D. S., Péton, V., de Almeida, S. S., Maréchal, C. L., Miyoshi, A., Azevedo, V., Berkova, N., Rault, L., François, P., Schrenzel, J., et al. (2012) · Journal of Bacteriology · doi:10.1128/jb.01188-12 · Cited by 29
Self-citationLactococcus garvieae endocarditis
Zuily, S., Mami, Z., & Meune, C. (2010) · Archives of cardiovascular diseases · doi:10.1016/j.acvd.2010.05.005 · Cited by 23
Frola, I. D., Pellegrino, M., Magnano, G., Giraudo, J., Espeche, M. C., Nader-Macı́as, M. E., & Bogni, C. I. (2012) · Journal of Dairy Research · doi:10.1017/s0022029912000581 · Cited by 22
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Cheng, W. N., & Han, S. G. (2020) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.20.0156 · Cited by 637
Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review
Sharun, K., Dhama, K., Tiwari, R., Gugjoo, M. B., Yatoo, M. I., Patel, S. K., Pathak, M., Karthik, K., Khurana, S. K., Singh, R., et al. (2021) · Veterinary Quarterly · doi:10.1080/01652176.2021.1882713 · Cited by 395
Derakhshani, H., Fehr, K. B., Sepehri, S., Francoz, D., Buck, J. D., Barkema, H. W., Plaizier, J. C., & Khafipour, E. (2018) · Journal of Dairy Science · doi:10.3168/jds.2018-14860 · Cited by 289
Mastitis treatment—Reduction in antibiotic usage in dairy cows
Krömker, V., & Leimbach, S. (2017) · Reproduction in Domestic Animals · doi:10.1111/rda.13032 · Cited by 264
Falentin, H., Rault, L., Nicolas, A., Bouchard, D. S., Lassalas, J., Lamberton, P., Aubry, J. M., Marnet, P., Loir, Y. L., & Even, S. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00480 · Cited by 198
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Metadata
- Authors
- Bouchard, D. S., Seridan, B., Saraoui, T., Rault, L., Germon, P., Gonzalez-Moreno, C., Nader-Macias, F. M. E., Baud, D., François, P., Chuat, V., Chain, F., Langella, P., Nicoli, J., Le Loir, Y., & Even, S.
- Affiliations
- Institut Agro Rennes-Angers (FR)
- Infectiologie et santé publique (FR)
- University Hospital of Geneva (CH)
- Microbiologie de l’alimentation au service de la santé (FR)
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Even, Sergine
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-12-29
- Volume
- 10
- Issue
- 12
- Pages
- e0144831
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4694705
- OpenAlex
- W2219521950
- Cited by
- 144
- References
- 40
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 23 · 2023: 15 · 2022: 15 · 2021: 20 · 2020: 16 · 2019: 6 · 2018: 12 · 2017: 15 · 2016: 7
- FWCI
- 24.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- HAL (submittedVersion, cc-by)
- Americanae (AECID Library) (submittedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-sa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Greater South Information System
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Institut National de la Recherche Agronomique
- Campus France
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Milk Quality and Mastitis in Dairy Cows
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Keywords
- bovine mastitis, lactic acid bacteria, Lactobacillus brevis, Lactobacillus plantarum, Lactococcus, mastitis prevention, competitive exclusion, anti-inflammatory activity, IL-8 secretion, Staphylococcus aureus, Escherichia coli, Streptococcus uberis, hydrophobicity, self-aggregation, antibiotic resistance genes, dairy cattle
- MeSH terms
- Animals, Bacterial Adhesion, Bacterial Infections, Cattle, Female, Humans, Lactobacillus, Mammary Glands, Animal, Mastitis, Bovine, Milk, Molecular Sequence Data, RNA, Bacterial, RNA, Ribosomal, 16S, Genome, Bacterial, Lactococcus, Sequence Analysis, DNA, HT29 Cells, Molecular Typing, Microbiota
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