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Evaluation of various methods of selection of B. subtilis strains capable of secreting surface-active compounds
Open accessAbstract
The aim of the study was the evaluation of a three-step method for the selection of bacterial strains capable of producing surfactin. The procedure consisted of the following steps: 1.blood agar test, 2. measurement of the surface tension (ST) of the medium using the du Nouy method before and after submerged culture, 3. qualitative and quantitative assessment of surfactin by HPLC. Forty five Bacillus subtilis natto strains producing haemolysis zones (≥3mm) were selected. Nineten of them reduced ST of the medium to ≤ 40 mN/m; in six cases, the reduction was as much as 50%. All indicated strains produced surfactin. Positive correlations (p <0.5) between the percentage reduction of ST of the medium and surfactin concentration (r = 0.44), indicate that this parameter is determinant of the ability to synthesize this compound. The blood agar test has been shown to be useful only as a pre-selection criterion for surfactin producers (18 strains selected by this method reduced ST by only ≤30%). The proposed selection strategy proved effective and made it possible to select the BS15 strain that reduced the ST of the medium to 30.56 ± 0.15 mN/m and simultaneously provided a high concentration of surfactin compared to other strains.
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![Fig 2 Surface tension (ST) [mN/m] of Cooper’s mineral medium measured before (Control) and after submerged cultivation (SmF) of selected Bacillus subtilis natto strains (BS15, BS19, BS21, BS25, BS35, BS36).](/paper-figures/21be766def6f4198bbcca25cdc43eef4/evaluation-of-various-methods-of-selection-of-b-subtilis-strains-capable-of-figure-2.jpg)
![Fig 3 Average concentration of surfactin [μg/mg] synthesized by selected strains (n = 3). Error bars represent standard deviation (SD).](/paper-figures/24fd1e332c8245b586d404e47c9c6e09/evaluation-of-various-methods-of-selection-of-b-subtilis-strains-capable-of-figure-3.jpg)
![Fig 6 Relationship between the decrease in surface tension [%] and the concentration of surfactin and its B isoform [μg/mg] in post-culture media of Bacillus subtilis natto strains (n = 63).](/paper-figures/d445472bc1ff4eda831fa72143829276/evaluation-of-various-methods-of-selection-of-b-subtilis-strains-capable-of-figure-6.jpg)
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“The biomass was dried to a constant mass using a weighting dryer (RADWAG WPS-30S, Radwag, Radom, Poland) at 105 ◦C and 20 s sampling time [60].”
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Metadata
- Authors
- Koim-Puchowska, B., Kłosowski, G., Mikulski, D., & Menka, A.
- Affiliations
- Kazimierz Wielki University in Bydgoszcz (PL)
- Corresponding author
- Kłosowski, Grzegorz
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2019-11-12
- Volume
- 14
- Issue
- 11
- Pages
- e0225108
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC6850892
- OpenAlex
- W2986642697
- Cited by
- 23
- References
- 67
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 2
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministerstwo Nauki i Szkolnictwa Wyższego (Grant No. 008/RID/2018/19)
- Topics
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Surfactants and Colloidal Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- Bacillus natto, surfactin, bacterial strain selection, surface tension reduction, hemolysis, submerged culture, biosurfactant production
- MeSH terms
- Bacillus subtilis, Hemolysis, Phenotype, Surface-Active Agents
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