The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Stability of circulating microRNAs in serum
Abstract
There is a strong body of evidence by several translational studies which demonstrate the potential of circulating miRNAs as a potential biomarker in oncology. However, recent reports documented varying stability of these small RNA molecules in serum samples. The aim of our pilot study was to evaluate the stability of miRNAs in serum in relation to food intake and sample storage. Serum miRNA expression levels of 16 different miRNAs from 8 healthy volunteers were quantified by real-time PCR. 4 samples from each donor were analysed—2 samples (fasting, in the morning and after food intake, at noon) were analysed within 24h and 2 samples (fasting and after food intake, at noon) were stored at -80°C for 14 days and subsequently analysed. Student´s t-test was used to determine significant differences. The detectability of the distinct miRNA as a surrogate for the stability of these small RNA molecules was slightly altered by the storage conditions, but only a miRNA 22-3p, out of the analysed 16 miRNAs, shows significant lower dCq expression (3.821 vs. 4.530; p<0,01) by qPCR dependent on storage conditions (-80°C vs. 4°C). However, miRNA levels were not affected by food intake. The difference between samples taken in the morning (fasting) and at noon (after a normal meal) did not show any significant differences. MiRNAs can be considered to be a relatively stable tool in laboratory diagnostics, but clearly every new assay needs thorough evaluation. The stability of miRNAs documented here in healthy volunteers shows their potential in the search for innovative biomarkers in oncology.
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“Unlike other RNA species, they are extracellularly stable and circulate in body fluids, including plasma and cerebrospinal fluid (CSF), and therefore, are discussed as biomarkers for various diseases [16–18].”
From Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability (2025) · cites this paper as [17]
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“miRNA is known to be exceptionally stable to degradation in the extracellular space, withstanding even harsh treatments such as numerous freeze-thaw cycles [17, 18].”
From Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability (2025) · cites this paper as [17]
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Cites
Circulating microRNAs as stable blood-based markers for cancer detection
Mitchell, P. S., Parkin, R. K., Kroh, E. M., Fritz, B. R., Wyman, S. K., Pogosova-Agadjanyan, E. L., Peterson, A., Noteboom, J., O'Briant, K. C., Allen, A., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0804549105 · Cited by 7,969
Kroh, E. M., Parkin, R. K., Mitchell, P. S., & Tewari, M. (2010) · Methods · doi:10.1016/j.ymeth.2010.01.032 · Cited by 1,184
A Brief Review on the Mechanisms of miRNA Regulation
Cai, Y., Yu, X., Hu, S., & Yu, J. M. (2009) · Genomics Proteomics & Bioinformatics · doi:10.1016/s1672-0229(08)60044-3 · Cited by 1,050
Liquid Biopsy: Current Status and Future Perspectives
Mader, S., & Pantel, K. (2017) · Oncology Research and Treatment · doi:10.1159/000478018 · Cited by 234
Köberle, V., Pleli, T., Schmithals, C., Alonso, E. A., Haupenthal, J., Bonig, H. B., Peveling‐Oberhag, J., Biondi, R. M., Zeuzem, S., Kronenberger, B., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0075184 · Cited by 194
Pre-profiling factors influencing serum microRNA levels
MacLellan, S. A., MacAulay, C. E., Lam, S. C., & Garnis, C. (2014) · BMC Clinical Pathology · doi:10.1186/1472-6890-14-27 · Cited by 106
Zhu, J., Zou, Z., Nie, P., Kou, X., Wu, B., Wang, S., Song, Z., & He, J. (2016) · Cell Death and Disease · doi:10.1038/cddis.2016.361 · Cited by 106
Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples
Matías‐García, P. R., Wilson, R., Mussack, V., Reischl, E., Waldenberger, M., Gieger, C., Anton, G., Peters, A., & Kühn-Steven, A. (2020) · PLoS ONE · doi:10.1371/journal.pone.0227648 · Cited by 106
Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer
Tsai, K.-W., Leung, C.-M., Lo, Y.-H., Chen, T.-W., Chan, W.-C., Yu, S.-Y., Tu, Y.-T., Lam, H.-C., Li, S.-C., Ger, L.-P., et al. (2016) · Scientific Reports · doi:10.1038/srep28176 · Cited by 84
Plasma MicroRNA Pair Panels as Novel Biomarkers for Detection of Early Stage Breast Cancer
Fang, R., Zhu, Y., Hu, L., Khadka, V. S., Ai, J., Zou, H., Ju, D., Jiang, B., Deng, Y., & Hu, X.-M. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2018.01879 · Cited by 62
Research works printed but not linked: 1
- J. Li, 2020, miR-28-3p promotes the malignant biological behaviors of triple negative breast cancer MDA-MB-468 cells via inhibiting BIN1, Chinese Journal of Cancer Biotherapy, 27, 55
Cited by
Kumbol, V., Ivanov, A., McGurran, H., Schüler, J., Zhai, Y., Ludwik, K., Hinkelmann, L., Brehm, M., Krüger, C., Küchler, J., et al. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02199-8 · Cited by 8
Maqueda, J. J., De Feo, A., & Scotlandi, K. (2024) · Biomolecules · doi:10.3390/biom14101306 · Cited by 4
Metadata
- Authors
- Kupec, T., Bleilevens, A., Iborra, S., Najjari, L., Wittenborn, J., Maurer, J., & Stickeler, E.
- ORCID
- Kupec, Tomas
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2022-08-31
- Volume
- 17
- Issue
- 8
- Pages
- e0268958
- ISSN
- 1932-6203
- PMC
- PMC9432728
- Cited by
- 60
- References
- 22
- Language
- en
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