The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Direct Visualization by Cryo-EM of the Mycobacterial Capsular Layer: A Labile Structure Containing ESX-1-Secreted Proteins
Open accessAbstract
The cell envelope of mycobacteria, a group of Gram positive bacteria, is composed of a plasma membrane and a Gram-negative-like outer membrane containing mycolic acids. In addition, the surface of the mycobacteria is coated with an ill-characterized layer of extractable, non-covalently linked glycans, lipids and proteins, collectively known as the capsule, whose occurrence is a matter of debate. By using plunge freezing cryo-electron microscopy technique, we were able to show that pathogenic mycobacteria produce a thick capsule, only present when the cells were grown under unperturbed conditions and easily removed by mild detergents. This detergent-labile capsule layer contains arabinomannan, alpha-glucan and oligomannosyl-capped glycolipids. Further immunogenic and proteomic analyses revealed that Mycobacterium marinum capsule contains high amounts of proteins that are secreted via the ESX-1 pathway. Finally, cell infection experiments demonstrated the importance of the capsule for binding to cells and dampening of pro-inflammatory cytokine response. Together, these results show a direct visualization of the mycobacterial capsular layer as a labile structure that contains ESX-1-secreted proteins.
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M. tuberculosis and M. leprae Translocate from the Phagolysosome to the Cytosol in Myeloid Cells
van der Wel, N., Hava, D., Houben, D., Fluitsma, D., van Zon, M., Pierson, J., Brenner, M., & Peters, P. J. (2007) · Cell · doi:10.1016/j.cell.2007.05.059 · Cited by 960
Self-citationPym, A. S., Brodin, P., Brosch, R., Huerre, M., & Cole, S. T. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03237.x · Cited by 729
Hoffmann, C., Leis, A., Niederweis, M., Plitzko, J. M., & Engelhardt, H. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709530105 · Cited by 596
Direct Visualization of the Outer Membrane of Mycobacteria and Corynebacteria in Their Native State
Zuber, B., Chami, M., Houssin, C., Dubochet, J., Griffiths, G., & Daffé, M. (2008) · Journal of Bacteriology · doi:10.1128/jb.01919-07 · Cited by 458
Systematic Genetic Nomenclature for Type VII Secretion Systems
Bitter, W., Houben, E. N. G., Bottai, D., Brodin, P., Brown, E. J., Cox, J. S., Derbyshire, K., Fortune, S. M., Gao, L.-Y., Liu, J., et al. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000507 · Cited by 307
Self-citationA mycobacterium ESX-1-secreted virulence factor with unique requirements for export.
McLaughlin, B., Chon, J. S., MacGurn, J. A., Carlsson, F., Cheng, T. L., Cox, J. S., & Brown, E. J. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030105 · Cited by 196
Cited by
Mycolic Acids: Structures, Biosynthesis, and Beyond
Marrakchi, H., Lanéelle, M.-A., & Daffé, M. (2014) · Chemistry & Biology · doi:10.1016/j.chembiol.2013.11.011 · Cited by 623
Self-citationBeckwith, K. S., Beckwith, M. S., Ullmann, S., Sætra, R. S., Kim, H., Marstad, A., Åsberg, S. E., Strand, T. A., Haug, M., Niederweis, M., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16143-6 · Cited by 282
Lindestam Arlehamn, C. S., Gerasimova, A., Mele, F., Henderson, R., Swann, J., Greenbaum, J. A., Kim, Y., Sidney, J., James, E. A., Taplitz, R., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003130 · Cited by 275
Pathogenesis, Immunology, and Diagnosis of Latent Mycobacterium tuberculosis Infection
Ahmad, S. (2011) · Clinical and Developmental Immunology · doi:10.1155/2011/814943 · Cited by 271
How sisters grow apart: mycobacterial growth and division
Kieser, K. J., & Rubin, E. J. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3299 · Cited by 268
Metadata
- Authors
- Sani, M., Houben, E. N. G., Geurtsen, J., Pierson, J., de Punder, K., van Zon, M., Wever, B., Piersma, S. R., Jiménez, C. R., Daffé, M., Appelmelk, B. J., Bitter, W., van der Wel, N., & Peters, P. J.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-03-05
- Volume
- 6
- Issue
- 3
- Pages
- e1000794
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- OpenAlex
- W2169882798
- Cited by
- 311
- References
- 6
- FWCI
- 11.34
- Type
- journal article
- Language
- en
- Open access
- gold
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- The paper
- Sani, M., Houben, E. N. G., Geurtsen, J., Pierson, J., de Punder, K., van Zon, M., Wever, B., Piersma, S. R., Jiménez, C. R., Daffé, M., Appelmelk, B. J., Bitter, W., van der Wel, N., & Peters, P. J. (2010). Direct Visualization by Cryo-EM of the Mycobacterial Capsular Layer: A Labile Structure Containing ESX-1-Secreted Proteins. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1000794
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