CoChat
Sign Up
Cited by 218Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchMalaria Research and Control

The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.

A Novel Family of Toxoplasma IMC Proteins Displays a Hierarchical Organization and Functions in Coordinating Parasite Division

Open access
Beck, Josh R., Rodriguez-Fernandez, Imilce A., Cruz de Leon, Jessica, Huynh, My-Hang, Carruthers, Vern B., Morrissette, Naomi S., Bradley, Peter J.·2010·PLoS Pathogens
DOI 10.1371/journal.ppat.1001094PMC PMC2936552
Open this paper in CoChat — free accountOpen PDF

Abstract

Apicomplexans employ a peripheral membrane system called the inner membrane complex (IMC) for critical processes such as host cell invasion and daughter cell formation. We have identified a family of proteins that define novel sub-compartments of the Toxoplasma gondii IMC. These IMC Sub-compartment Proteins, ISP1, 2 and 3, are conserved throughout the Apicomplexa, but do not appear to be present outside the phylum. ISP1 localizes to the apical cap portion of the IMC, while ISP2 localizes to a central IMC region and ISP3 localizes to a central plus basal region of the complex. Targeting of all three ISPs is dependent upon N-terminal residues predicted for coordinated myristoylation and palmitoylation. Surprisingly, we show that disruption of ISP1 results in a dramatic relocalization of ISP2 and ISP3 to the apical cap. Although the N-terminal region of ISP1 is necessary and sufficient for apical cap targeting, exclusion of other family members requires the remaining C-terminal region of the protein. This gate-keeping function of ISP1 reveals an unprecedented mechanism of interactive and hierarchical targeting of proteins to establish these unique sub-compartments in the Toxoplasma IMC. Finally, we show that loss of ISP2 results in severe defects in daughter cell formation during endodyogeny, indicating a role for the ISP proteins in coordinating this unique process of Toxoplasma replication.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.ppat.1001094

Cites

61 of 61 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Show all 61 references

Research works printed but not linked: 1

  1. E Porchet, 1977, [Freeze fracture study of Toxoplasma and Sarcocystis infective stages (author's transl)]., Z Parasitenkd, 54, 101

Cited by

Sorted by citations

Items 201–218 of 218

Related

See all

Metadata

Authors
Beck, J. R., Rodriguez-Fernandez, I. A., Cruz de Leon, J., Huynh, M.-H., Carruthers, V. B., Morrissette, N. S., & Bradley, P. J.
Affiliations
  • University of California, Los Angeles (US)
  • University of Michigan (US)
  • University of California, Irvine (US)
Corresponding author
Bradley, Peter J.
ORCID
Venue
PLoS Pathogens
Publisher
Public Library of Science (PLoS)
Published
2010-09-09
Volume
6
Issue
9
Pages
e1001094
ISSN
  • 1553-7366 (ISSN-L)
  • 1553-7374
DOI
10.1371/journal.ppat.1001094
PMC
PMC2936552
OpenAlex
W1996756350
Cited by
217
References
61
Citations by year
2026: 4 · 2025: 17 · 2024: 15 · 2023: 13 · 2022: 10 · 2021: 22 · 2020: 15 · 2019: 20 · 2018: 8 · 2017: 14 · 2016: 17 · 2015: 15 · 2014: 12 · 2013: 12 · 2012: 13
FWCI
8.62
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Pathogens (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • Figshare (submittedVersion, cc-by)
Funders
  • National Institutes of Health (4r01ai064616-10, NIH-R01)
  • U.S. Public Health Service (R01 #A121423)
  • National Institute of Allergy and Infectious Diseases (R01 #AI064616, T32AI07323, R01AI067981, R01AI046675, T32AI007323)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Keywords
Toxoplasma gondii, inner membrane complex, apical cap, protein subcellular targeting, myristoylation, palmitoylation, endodyogeny, cell division, Apicomplexa, parasite replication
MeSH terms
Amino Acid Sequence, Animals, Cell Division, Cell Membrane, Cells, Cultured, Fibroblasts, Humans, Immunoglobulin G, Immunization, Male, Membrane Proteins, Mice, Inbred BALB C, Molecular Sequence Data, RNA, Messenger, Toxoplasma, Toxoplasmosis, Blotting, Western, Protozoan Proteins, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, Reverse Transcriptase Polymerase Chain Reaction, Mice, Foreskin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review