Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming
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Campylobacter jejuni is the major cause of bacterial food-borne illness in the USA and Europe. An important virulence attribute of this bacterial pathogen is its ability to enter and survive within host cells. Here we show through a quantitative proteomic analysis that upon entry into host cells, C. jejuni undergoes a significant metabolic downshift. Furthermore, our results indicate that intracellular C. jejuni reprograms its respiration, favoring the respiration of fumarate. These results explain the poor ability of C. jejuni obtained from infected cells to grow under standard laboratory conditions and provide the bases for the development of novel anti microbial strategies that would target relevant metabolic pathways.
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Nitrate reduction is an important pathway for Campylobacter during host colonization.
“It has recently been demonstrated that nitrate reduction is an important pathway for Campylobacter during host colonization.”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [74]
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- Authors
- Liu, X., Gao, B., Novik, V., & Galán, J. E.
- Affiliations
- Yale University (US)
- Harvard University (US)
- Corresponding author
- Galán, Jorge E.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-03-08
- Volume
- 8
- Issue
- 3
- Pages
- e1002562
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- PMC
- PMC3297583
- OpenAlex
- W2029470813
- Cited by
- 69
- References
- 34
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 2 · 2020: 5 · 2019: 4 · 2018: 7 · 2017: 8 · 2016: 4 · 2015: 6 · 2014: 13 · 2013: 8 · 2012: 1
- FWCI
- 13.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS Pathogens (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Ellison Medical Foundation
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Surgery › Helicobacter pylori-related gastroenterology studies
- Keywords
- Campylobacter jejuni, intracellular survival, host cell invasion, metabolic reprogramming, fumarate respiration, bacterial metabolism, food poisoning, antimicrobial drug targets, host-pathogen interaction
- MeSH terms
- Animals, Bacterial Proteins, Chlorocebus aethiops, Energy Metabolism, Food Contamination, Fumarates, Gene Expression Regulation, Campylobacter jejuni, Bacterial Physiological Phenomena, COS Cells, Proteomics, Host-Pathogen Interactions, Metabolome, Cellular Reprogramming
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