Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress
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Summary: Pathways of electron transport to periplasmic nitrate (NapA) and nitrite (NrfA) reductases have been investigated in Campylobacter jejuni, a microaerophilic food‐borne pathogen. The nap operon is unusual in lacking napC (encoding a tetra‐haem c‐type cytochrome) and napF, but contains a novel gene of unknown function, napL. The iron‐sulphur protein NapG has a major role in electron transfer to the NapAB complex, but we show that slow nitrate‐dependent growth of a napG mutant can be sustained by electron transfer from NrfH, the electron donor to the nitrite reductase NrfA. A napL mutant possessed ∼50% lower NapA activity than the wild type but showed normal growth with nitrate as the electron acceptor. NrfA was constitutive and was shown to play a role in protection against nitrosative stress in addition to the previously identified NO‐inducible single domain globin, Cgb. However, nitrite also induced cgb expression in an NssR‐dependent manner, suggesting that growth of C. jejuni with nitrite causes nitrosative stress. This was confirmed by lack of growth of cgb and nssR mutants, and slow growth of the nrfA mutant, in media containing nitrite. Thus, NrfA and Cgb together provide C. jejuni with constitutive and inducible components of a robust defence against nitrosative stress.
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Mutation of napL in W. succinogenes and C. jejuni delays respiratory growth on nitrate media.
“While the function of NapL is unknown, mutation of napL in W. succinogenes and C. jejuni delays respiratory growth on nitrate media.105,276”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [276]
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Nitrate and periplasmic nitrate reductases
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- Authors
- Pittman, M. S., Elvers, K. T., Lee, L., Jones, M. A., Poole, R. K., Park, S. F., & Kelly, D. J.
- Affiliations
- University of Sheffield (GB)
- University of Surrey (GB)
- University of Nottingham (GB)
- Corresponding author
- Kelly, David J.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2006-11-21
- Volume
- 63
- Issue
- 2
- Pages
- 575-590
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1500585787
- Cited by
- 131
- References
- 50
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 10 · 2020: 4 · 2019: 8 · 2018: 2 · 2017: 5 · 2016: 6 · 2015: 4 · 2014: 13 · 2013: 11 · 2012: 6
- FWCI
- 5.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Open Research (University of Surrey) (submittedVersion)
- Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/E010504/1, D18189)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- Campylobacter jejuni, nitrate reductase, nitrite reductase, napA, nrfA, non-aqueous phase liquids, electron transport, nitrosative stress, nitrite, nitrate, iron-sulfur proteins
- MeSH terms
- Adaptation, Physiological, Animals, Anti-Bacterial Agents, Bacterial Proteins, beta-Galactosidase, Chickens, Electron Transport, Multigene Family, Hemoglobins, Microbial Sensitivity Tests, Models, Biological, Nitrates, Nitrites, Oxidoreductases, Blotting, Western, Gene Expression Regulation, Bacterial, Campylobacter jejuni, Mutagenesis, Insertional, Gene Deletion, Artificial Gene Fusion, Nitric Oxide Donors, Microbial Viability, Truncated Hemoglobins
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