The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)
Abstract
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Immunoassay of endogenous plasma insulin in man
Yalow, R. S., & Berson, S. A. (1960) · Journal of Clinical Investigation · doi:10.1172/jci104130 · Cited by 3,041
Cited by
Wudy, S. A., Schuler, G., Sánchez-Guijo, A., & Hartmann, M. F. (2018) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2017.09.003 · Cited by 236
FRET Based Biosensor: Principle Applications Recent Advances and Challenges
Verma, A. K., Noumani, A., Yadav, A. K., & Solanki, P. R. (2023) · Diagnostics · doi:10.3390/diagnostics13081375 · Cited by 202
Ghallab, N. A. (2018) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2017.12.022 · Cited by 174
Henrique, A. J., Gabrielloni, M. C., Rodney, P., & Barbieri, M. (2018) · International Journal of Nursing Practice · doi:10.1111/ijn.12642 · Cited by 69
Bao, R.-M., Yang, H.-M., Yu, C.-M., Zhang, W.-F., & Tang, J.-B. (2016) · Protein Expression and Purification · doi:10.1016/j.pep.2016.05.007 · Cited by 31
Metadata
- Authors
- Lequin, R. M.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-12-01
- Volume
- 51
- Issue
- 12
- Pages
- 2415-2418
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2161863487
- Cited by
- 1,548
- References
- 1
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). https://app.cochat.ai/papers/10.1373/clinchem.2005.051532
- The paper
- Lequin, R. M. (2005). Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). Clinical Chemistry. https://doi.org/10.1373/clinchem.2005.051532
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
