The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Metagenomic Pyrosequencing and Microbial Identification
Open accessAbstract
Background: The Human Microbiome Project has ushered in a new era for human metagenomics and high-throughput next-generation sequencing strategies. Content: This review describes evolving strategies in metagenomics, with a special emphasis on the core technology of DNA pyrosequencing. The challenges of microbial identification in the context of microbial populations are discussed. The development of next-generation pyrosequencing strategies and the technical hurdles confronting these methodologies are addressed. Bioinformatics-related topics include taxonomic systems, sequence databases, sequence-alignment tools, and classifiers. DNA sequencing based on 16S rRNA genes or entire genomes is summarized with respect to potential pyrosequencing applications. Summary: Both the approach of 16S rDNA amplicon sequencing and the whole-genome sequencing approach may be useful for human metagenomics, and numerous bioinformatics tools are being deployed to tackle such vast amounts of microbiological sequence diversity. Metagenomics, or genetic studies of microbial communities, may ultimately contribute to a more comprehensive understanding of human health, disease susceptibilities, and the pathophysiology of infectious and immune-mediated diseases.
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Cites
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
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Host-Bacterial Mutualism in the Human Intestine
Bäckhed, F., Ley, R. E., Sonnenburg, J. L., Peterson, D. A., & Gordon, J. I. (2005) · Science · doi:10.1126/science.1104816 · Cited by 5,277
Ecological and Evolutionary Forces Shaping Microbial Diversity in the Human Intestine
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Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach
Manichanh, C., Rigottier-Gois, L., Bonnaud, E., Gloux, K., Pelletier, E., Frangeul, L., Nalin, R., Jarrin, C., Chardon, P., Marteau, P., et al. (2006) · Gut · doi:10.1136/gut.2005.073817 · Cited by 2,277
The complete genome of an individual by massively parallel DNA sequencing
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Self-citationFlanagan, J. L., Brodie, E. L., Weng, L., Lynch, S. V., Garcia, O., Brown, R., Hugenholtz, P., DeSantis, T. Z., Andersen, G. L., Wiener-Kronish, J. P., et al. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.02187-06 · Cited by 180
Cited by
Sequencing technologies – the next generation
Metzker, M. L. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2626 · Cited by 7,066
Romero, R., Hassan, S. S., Gajer, P., Tarca, A. L., Fadrosh, D. W., Nikita, L., Galuppi, M., Lamont, R. F., Chaemsaithong, P., Miranda, J., et al. (2014) · Microbiome · doi:10.1186/2049-2618-2-4 · Cited by 852
The gut microbiota, obesity and insulin resistance
Shen, J., Obin, M. S., & Zhao, L. (2013) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2012.11.001 · Cited by 599
DNA Methylation Analysis by Pyrosequencing
Busato, F., & Tost, J. (2014) · Encyclopedia of Metagenomics · doi:10.1007/978-1-4614-6418-1_799-1 · Cited by 499
Dysbiosis Signature of Fecal Microbiota in Colorectal Cancer Patients
Wu, N., Yang, X., Zhang, R., Li, J., Xiao, X., Hu, Y., Chen, Y., Yang, F., Lu, N., Wang, Z., et al. (2013) · Microbial Ecology · doi:10.1007/s00248-013-0245-9 · Cited by 454
Metadata
- Authors
- Petrosino, J. F., Highlander, S., Luna, R. A., Gibbs, R. A., & Versalovic, J.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-05-01
- Volume
- 55
- Issue
- 5
- Pages
- 856-866
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2158877985
- Cited by
- 531
- References
- 6
- FWCI
- 17.76
- Type
- journal article
- Language
- en
- Open access
- green
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- Petrosino, J. F., Highlander, S., Luna, R. A., Gibbs, R. A., & Versalovic, J. (2009). Metagenomic Pyrosequencing and Microbial Identification. Clinical Chemistry. https://doi.org/10.1373/clinchem.2008.107565
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