Cited by 314Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsAdvanced Biosensing Techniques and Applications

The DOI matches its Crossref record; 33 of the 34 references it cites were found in a registry or an index.

Quantification of Thyroglobulin, a Low-Abundance Serum Protein, by Immunoaffinity Peptide Enrichment and Tandem Mass Spectrometry

Open access

Hoofnagle, Andrew N, Becker, Jessica O, Wener, Mark H, Heinecke, Jay W·2008·Clinical Chemistry

DOI 10.1373/clinchem.2008.109652PMC PMC2739673

Open this paper in CoChat — free account Open PDF

Abstract

Background: Quantification of serum tumor markers plays an important role in determining whether patients treated for cancer require further therapy. Whereas large-scale proteomic efforts aim to identify novel tumor markers to facilitate early detection, optimization of methods for quantifying known tumor markers offers another approach to improving management of malignancies. For example, immunoassays used in clinical practice to measure established tumor markers suffer from potential interference from endogenous immunoglobulins and imperfect concordance across platforms—problems that also plague many other immunoassays. To address these important limitations, this study used peptide immunoaffinity enrichment in concert with liquid chromatography–tandem mass spectrometry (LC-MS/MS) to quantify thyroglobulin, a well-characterized tumor marker. Methods: We identified 3 peptides in tryptic digests of thyroglobulin that were detected at low concentrations by tandem mass spectrometry, raised polyclonal antibodies to those peptides, and used the antibodies to extract the 3 corresponding peptides from tryptic digests of human serum. We quantified each endogenous peptide using LC-MS/MS and multiple reaction monitoring with external calibrators. Results: The detection limit for endogenous thyroglobulin in serum was 2.6 μg/L (4 pmol/L). Direct comparison with immunoassay revealed good correlation (r2 = 0.81). Conclusions: Immunoaffinity peptide enrichment–tandem mass spectrometry can detect tryptic peptides of thyroglobulin at picomolar concentrations while also digesting the endogenous immunoglobulins that can potentially interfere with traditional immunoassays. Our observations suggest a general analytical strategy for using immunoaffinity isolation together with tandem mass spectrometry to quantify tumor antigens and other low-abundance proteins in human serum.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1373/clinchem.2008.109652

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Protein biomarker discovery and validation: the long and uncertain path to clinical utility
Rifai, N., Gillette, M. A., & Carr, S. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1235 · Cited by 1,867 · cited 1×
“There is intense interest in using proteomics to discovernovelbiomarkersforthediagnosisandprognosis ofdisease;however,validationhasprovedtobeamajor stumblingblock( 1 ).Althoughthefinalaimofbiomarker discovery efforts is the development of a novel quantitative clinical assay, current immunoassay methodologies suffer fr…”
Background
Laboratory Support for the Diagnosis and Monitoring of Thyroid Disease
(2003) · Thyroid · doi:10.1089/105072503321086962 · Cited by 1,212
Mass Spectrometric Quantitation of Peptides and Proteins Using Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA)
Anderson, N. L., Anderson, N. G., Haines, L. R., Hardie, D. B., Olafson, R. W., & Pearson, T. W. (2004) · Journal of Proteome Research · doi:10.1021/pr034086h · Cited by 806 · cited 2×
“We thereforetestedamethoddevelopedbyAndersonetal. ( 22 ), termed “stable isotope standards and capture by antipeptide antibodies,” that combines immunoaffinity peptide purification with tandem mass spectrometry.”
Background
Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution
Keshishian, H., Addona, T. A., Burgess, M. W., Kuhn, E. W., & Carr, S. A. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700354-mcp200 · Cited by 686 · cited 1×
“We therefore optimized the digestion of whole serum for the immunoaffinity enrichmentofTgpeptides.Oneimportantsourceofvariabilityinproteomicanalysesispartialproteolyticdigestion ofcomplexbiologicalmaterial.Highconcentrationsof chaotropic salts are commonly used to denature proteins and help facilitate more complete tr…”
Method
ELISA Guidebook, The
Crowther, J. R. (2000) · Humana Press eBooks · doi:10.1385/1592590497 · Cited by 517
Serum Thyroglobulin Autoantibodies: Prevalence, Influence on Serum Thyroglobulin Measurement, and Prognostic Significance in Patients with Differentiated Thyroid Carcinoma1
Spencer, C. A., Takeuchi, M., Kazarosyan, M., Wang, C. C., Guttler, R., SINGER, P. A., Fatemi, S., LoPresti, J. S., & Nicoloff, J. T. (1998) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.83.4.4683 · Cited by 476 · cited 1×
“Serum Tg has long been a thorn in the side of clinical tumor marker immunoassays because of its low concentration and common interferences (8, 10 , 11, 13, 26).”
Background
Laboratory medicine practice guidelines: laboratory support for the diagnosis and monitoring of thyroid disease
Demers, L. M., & Spencer, C. A. (2003) · Clinical Endocrinology · doi:10.1046/j.1365-2265.2003.01681.x · Cited by 460
Quantification of C‐reactive protein in the serum of patients with rheumatoid arthritis using multiple reaction monitoring mass spectrometry and 13C‐labeled peptide standards
Kuhn, E. W., Wu, J., Karl, J., Liao, H., Zolg, W., & Guild, B. (2004) · PROTEOMICS · doi:10.1002/pmic.200300670 · Cited by 402 · cited 2×
“To test a general approach to detecting low-abundance proteins in serum that avoids the limitations inherent to immunoassays, we took advantage of the power of mass spectrometry (MS) to detect specific peptidesincomplexmixtures.Previousattemptstouse MS to quantify low-abundance endogenous serum proteins include the di…”
Background
Isotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I
Barr, J. R., Maggio, V. L., Patterson, D. G., Cooper, G. R., Henderson, L. O., Turner, W. E., Smith, S. J., Hannon, W. H., Needham, L. L., & Sampson, E. J. (1996) · Clinical Chemistry · doi:10.1093/clinchem/42.10.1676 · Cited by 347
A Single Recombinant Human Thyrotropin-Stimulated Serum Thyroglobulin Measurement Predicts Differentiated Thyroid Carcinoma Metastases Three to Five Years Later
Kloos, R. T., & Mazzaferri, E. L. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-0492 · Cited by 254

Flagged first, then by citations

Show all 34 references

1 of the 34 references couldn’t be matched to a known paper.

Research works printed but not linked: 4

1998, J Clin Endocrinol Metab, 83, 1121
1996, Ann Biol Clin (Paris), 54, 343
2006, Clin Lab Int, 8, 12
2001, The Elisa Guidebook, 421 p

Sorted by citations

Items 101–200 of 314

Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry for Peptide and Protein Quantification
Whiteaker, J. R., & Paulovich, A. G. (2011) · Clinics in Laboratory Medicine · doi:10.1016/j.cll.2011.07.004 · Cited by 48
Targeted human cerebrospinal fluid proteomics for the validation of multiple Alzheimer's disease biomarker candidates
Choi, Y. S., Hou, S., Choe, L. H., & Lee, K. H. (2013) · Journal of Chromatography B · doi:10.1016/j.jchromb.2013.05.003 · Cited by 47
Sample preparation strategies for targeted proteomics via proteotypic peptides in human blood using liquid chromatography tandem mass spectrometry
Dittrich, J., Becker, S., Hecht, M., & Ceglarek, U. (2014) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201400121 · Cited by 50
Six decades searching for meaning in the proteome
Anderson, L. (2014) · Journal of Proteomics · doi:10.1016/j.jprot.2014.03.005 · Cited by 47
Clinical translation of MS-based, quantitative plasma proteomics: status, challenges, requirements, and potential
Percy, A. J., Byrns, S., Pennington, S. R., Holmes, D. T., Anderson, N. L., Agreste, T. M., & Duffy, M. A. (2016) · Expert Review of Proteomics · doi:10.1080/14789450.2016.1205950 · Cited by 47
“date the peptide-capture approach has been successfully applied to the clinical measurement of thyroglobulin [70,71], a marker for thyroid cancer recurrence in which endogenous”
Method
Determination of the concentration range for 267 proteins from 21 lots of commercial human plasma using highly multiplexed multiple reaction monitoring mass spectrometry
Gaither, C., Popp, R., Mohammed, Y., & Borchers, C. H. (2020) · The Analyst · doi:10.1039/c9an01893j · Cited by 48
Multiplexing Determination of Small Cell Lung Cancer Biomarkers and Their Isovariants in Serum by Immunocapture LC-MS/MS
Torsetnes, S. B., Levernæs, M. C. S., Broughton, M. N., Paus, E., Halvorsen, T. G., & Reubsaet, L. (2014) · Analytical Chemistry · doi:10.1021/ac500986t · Cited by 46
Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation
Chen, L., Roos, D., & Philip, E. (2016) · Journal of Immunology Research · doi:10.1155/2016/7682472 · Cited by 46
“Immunopurifica-tion can be done either at the protein-level prior to digestion [40, 41] or at the peptide level after digestion [42].”
Immunocapture strategies in translational proteomics
Fredolini, C., Byström, S., Pin, E., Edfors, F., Tamburro, D., Iglesias, M. J., Månberg, A., Hong, M., Uhlén, M., Nilsson, P., et al. (2015) · Expert Review of Proteomics · doi:10.1586/14789450.2016.1111141 · Cited by 47
Quantification of biopharmaceuticals and biomarkers in complex biological matrices: a comparison of liquid chromatography coupled to tandem mass spectrometry and ligand binding assays
Bults, P., van de Merbel, N. C., & Bischoff, R. (2015) · Expert Review of Proteomics · doi:10.1586/14789450.2015.1050384 · Cited by 46
Photonic Technologies for Liquid Biopsies: Recent Advances and Open Research Challenges
Dell’Olio, F., Su, J., Huser, T. R., Sottile, V., Cortés‐Hernández, L. E., & Alix‐Panabières, C. (2020) · Laser & Photonics Review · doi:10.1002/lpor.202000255 · Cited by 44
Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis
Shi, T., Fillmore, T., Gao, Y., Zhao, R., He, J., Schepmoes, A., Nicora, C., Wu, C., Chambers, J., Moore, R., et al. (2013) · Analytical Chemistry · doi:10.1021/ac402105s · Cited by 43
Catch and measure–mass spectrometry‐based immunoassays in biomarker research
Weiß, F., van den Berg, B. H. J., Planatscher, H., Pynn, C. J., Joos, T. O., & Poetz, O. (2013) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2013.09.010 · Cited by 42
Development of a Protein Standard Absolute Quantification (PSAQ™) assay for the quantification of Staphylococcus aureus enterotoxin A in serum
Adrait, A., Lebert, D., Trauchessec, M., Dupuis, A., Louwagie, M., Masselon, C., Jaquinod, M., Chevalier, B., Vandenesch, F., Garin, J., et al. (2011) · Journal of Proteomics · doi:10.1016/j.jprot.2011.11.031 · Cited by 41
Simultaneous Phenotyping and Quantification of α-1-Antitrypsin by Liquid Chromatography–Tandem Mass Spectrometry
Chen, Y., Snyder, M. R., Zhu, Y., Tostrud, L. J., Benson, L. M., Katzmann, J. A., & Bergen, H. R. (2011) · Clinical Chemistry · doi:10.1373/clinchem.2011.163006 · Cited by 41
Quantitative Analysis of Prostate Specific Antigen Isoforms Using Immunoprecipitation and Stable Isotope Labeling Mass Spectrometry
Chen, Y., Tuan, L.-P., Chen, H., Wei, I.-A., Chou, M., Chen, H., Tyan, Y., & Chen, S. (2014) · Analytical Chemistry · doi:10.1021/ac5033066 · Cited by 41
Thyroglobulin in differentiated thyroid cancer
Evans, C., Tennant, S., & Perros, P. (2014) · Clinica Chimica Acta · doi:10.1016/j.cca.2014.10.035 · Cited by 40
HILIC–MRM–MS for Linkage-Specific Separation of Sialylated Glycopeptides to Quantify Prostate-Specific Antigen Proteoforms
van der Burgt, Y. E. M., Siliakus, K. M., Cobbaert, C. M., & Ruhaak, L. R. (2020) · Journal of Proteome Research · doi:10.1021/acs.jproteome.0c00050 · Cited by 41
Introduction: Advances in Protein Analysis for the Clinical Laboratory
Hortin, G. L., Carr, S. A., & Anderson, N. L. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2009.132803 · Cited by 40
Reconstructing the pipeline by introducing multiplexed multiple reaction monitoring mass spectrometry for cancer biomarker verification: An NCI‐CPTC initiative perspective
Rodriguez, H., Rivers, R. C., Kinsinger, C. R., Mesri, M., Hiltke, T., Rahbar, A. M., & Boja, E. S. (2010) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201000057 · Cited by 39
“Moreover, the capability for simultaneous measurement and characterization of targeted analytes allows three important advances in protein assays, on which the mock 510(k) PepCa10 test is based [29]: (i) it permits facile multiplexed measurement of many molecular species without significant interference; (ii) it facil…”
Background
Quantification of peptides from immunoglobulin constant and variable regions by LC‐MRM MS for assessment of multiple myeloma patients
Remily-Wood, E. R., Benson, K., Baz, R., Chen, Y. A., Hussein, M. A., Hartley-Brown, M. A., Sprung, R. W., Perez, B., Liu, R. Z., Yoder, S., et al. (2014) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201300077 · Cited by 39
Clinical applications of MS‐based protein quantification
Sabbagh, B., Mindt, S., Neumaier, M., & Findeisen, P. (2016) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201500116 · Cited by 41
Quantitation of Human Peptides and Proteins Via MS: Review of Analytically Validated Assays
Chappell, D. L., Lassman, M. E., McAvoy, T., Lin, M., Spellman, D. S., & Laterza, O. (2014) · Bioanalysis · doi:10.4155/bio.14.145 · Cited by 38
Automated Microchromatography Enables Multiplexing of Immunoaffinity Enrichment of Peptides to Greater than 150 for Targeted MS-Based Assays
Ippoliti, P. J., Kuhn, E. W., Mani, D. R., Fagbami, L., Keshishian, H., Burgess, M. W., Jaffe, J. D., & Carr, S. A. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b00946 · Cited by 41
Improved detection specificity for plasma proteins by targeting cysteine-containing peptides with photo-SRM
Enjalbert, Q., Girod, M., Simon, R., Jeudy, J., Chirot, F., Salvador, A., Antoine, R., Dugourd, P., & Lemoine, J. (2013) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-012-6603-5 · Cited by 37
“The value of SRM-based absolute quantification has been now widely illustrated for numerous relatively abundant plasma proteins or few weakly concentrated biomarkers and good correlations with the referent immunoassays have been demonstrated [5-10].”
Background
Advances in Clinical Mass Spectrometry
French, D. (2016) · Advances in clinical chemistry · doi:10.1016/bs.acc.2016.09.003 · Cited by 36
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Zhao, L., Whiteaker, J. R., Pope, M. E., Kuhn, E. W., Jackson, A., Anderson, N. L., Pearson, T. W., Carr, S. A., & Paulovich, A. G. (2011) · Journal of Visualized Experiments · doi:10.3791/2812 · Cited by 36
“The reagents are relatively less expensive to generate, the specificity for the analyte is excellent, the assays can be highly multiplexed, enrichment can be performed from neat plasma (no depletion required), and the technique is amenable to a wide array of proteins or modifications of interest.(8-13) In this video w…”
Method
Rapid Verification of Candidate Serological Biomarkers Using Gel-based, Label-free Multiple Reaction Monitoring
Tang, H., Beer, L. A., Barnhart, K. T., & Speicher, D. W. (2011) · Journal of Proteome Research · doi:10.1021/pr2002098 · Cited by 35
Rapid Multiplexed Proteomic Screening for Primary Immunodeficiency Disorders From Dried Blood Spots
Collins, C. J., Chang, I. J., Jung, S., Dayuha, R., Whiteaker, J. R., Segundo, G. R. S., Torgerson, T. R., Ochs, H. D., Paulovich, A. G., & Hahn, S. H. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02756 · Cited by 35
“Affinity-purified polyclonal antibodies (Pacific Immunology, Ramona, CA) were generated against all five peptides and pursued for use in human samples because of their ability to successfully capture their target sequences and the absence of background signals brought on by copurified peptide contaminants (26, 28, 39).”
Background
Exploring the Complementary Selectivity of Immunocapture and MS Detection for the Differentiation between hCG Isoforms in Clinically Relevant Samples
Lund, H., Torsetnes, S. B., Paus, E., Nustad, K., Reubsaet, L., & Halvorsen, T. G. (2009) · Journal of Proteome Research · doi:10.1021/pr900580n · Cited by 35
Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G., & Paulovich, A. G. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.o114.043133 · Cited by 34
Clinical peptide and protein quantification by mass spectrometry (MS)
Grebe, S. K. G., & Singh, R. J. (2016) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2016.01.026 · Cited by 35
High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays
Whiteaker, J. R., Zhao, L., Frisch, C., Ylera, F., Harth, S., Knappik, A., & Paulovich, A. G. (2014) · Journal of Proteome Research · doi:10.1021/pr4009404 · Cited by 34
Calibration Using a Single-Point External Reference Material Harmonizes Quantitative Mass Spectrometry Proteomics Data between Platforms and Laboratories
Pino, L. K., Searle, B. C., Huang, E. L., Noble, W. S., Hoofnagle, A. N., & MacCoss, M. J. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b04581 · Cited by 34
Self-citation
Methods for Identification of CA125 from Ovarian Cancer Ascites by High Resolution Mass Spectrometry
Weiland, F., Fritz, K., Oehler, M. K., & Hoffmann, P. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13089942 · Cited by 34
“As a way to overcome potential interferences of CA125 ELISAs with other proteins, an MRM mass spectrometric approach could be applied [30].”
Laboratory Thyroid Tests: A Historical Perspective
Spencer, C. A. (2023) · Thyroid · doi:10.1089/thy.2022.0397 · Cited by 34
An Inflection Point in Cancer Protein Biomarkers: What was and What's Next
Barker, A. D., Alba, M. M., Mallick, P., Agus, D. B., & Lee, J. S. H. (2023) · Molecular & Cellular Proteomics · doi:10.1016/j.mcpro.2023.100569 · Cited by 34
Clinical Protein Science and Bioanalytical Mass Spectrometry with an Emphasis on Lung Cancer
Végvári, Á., & Marko‐Varga, G. (2010) · Chemical Reviews · doi:10.1021/cr100011x · Cited by 33
Multiplexed Proteomic Analysis for Diagnosis and Screening of Five Primary Immunodeficiency Disorders From Dried Blood Spots
Collins, C. J., Yi, F., Dayuha, R., Whiteaker, J. R., Ochs, H. D., Freeman, A. F., Su, H. C., Paulovich, A. G., Segundo, G. R. S., Torgerson, T. R., et al. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.00464 · Cited by 33
Usefulness of a Thyroglobulin Liquid Chromatography–Tandem Mass Spectrometry Assay for Evaluation of Suspected Heterophile Interference
Netzel, B. C., Grebe, S. K. G., & Algeciras‐Schimnich, A. (2014) · Clinical Chemistry · doi:10.1373/clinchem.2014.224816 · Cited by 33
Manipulating trypsin digestion conditions to accelerate proteolysis and simplify digestion workflows in development of protein mass spectrometric assays for the clinical laboratory
Zheng, Y. Z., & DeMarco, M. L. (2017) · Clinical mass spectrometry · doi:10.1016/j.clinms.2017.10.001 · Cited by 32
Development and Validation of Apolipoprotein AI-Associated Lipoprotein Proteome Panel for the Prediction of Cholesterol Efflux Capacity and Coronary Artery Disease
Jin, Z., Collier, T. S., Dai, D. L., Chen, V., Hollander, Z., Ng, R. T., McManus, B. M., Balshaw, R. F., Apostolidou, S., Penn, M. S., et al. (2018) · Clinical Chemistry · doi:10.1373/clinchem.2018.291922 · Cited by 33
Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation
Bischoff, R., Bronsema, K. J., van de Merbel, N. C., Bronsema, K. J., & van de Merbel, N. C. (2013) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2012.11.015 · Cited by 32
Measurement of Lipoprotein-Associated Phospholipase A2 by Use of 3 Different Methods: Exploration of Discordance between ELISA and Activity Assays
Topbas, C., Swick, A. R., Razavi, M., Anderson, N. L., Pearson, T. W., & Bystrom, C. E. (2018) · Clinical Chemistry · doi:10.1373/clinchem.2017.279752 · Cited by 32
Immunoproteomics Using Polyclonal Antibodies and Stable Isotope–labeled Affinity-purified Recombinant Proteins
Edfors, F., Boström, T., Forsström, B., Zeiler, M., Johansson, H. J., Lundberg, E. K., Hober, S., Lehtiö, J., Mann, M., & Uhlén, M. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.034140 · Cited by 31
Concordance between thyroglobulin antibody assays
Taylor, K., Parkington, D. A., Bradbury, S., Simpson, H., Jefferies, S., & Halsall, D. J. (2011) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1258/acb.2011.010248 · Cited by 29
Mass-Linked Immuno-Selective Assays in Targeted Proteomics
Madian, A. G., Rochelle, N. S., & Regnier, F. E. (2012) · Analytical Chemistry · doi:10.1021/ac302071k · Cited by 29
Development and Multiple Validation of the Protein Multi-marker Panel for Diagnosis of Pancreatic Cancer
Kim, Y., Yeo, I., Huh, I., Kim, J., Han, D., Jang, J., & Kim, Y. (2021) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-20-3929 · Cited by 28
“One particular MRM-MS assay that measures thyroglobulin, a biomarker for monitoring thyroid carcinoma, is being used in six clinical laboratories throughout North America (31).”
Background
Quantitation of the Dynamic Profiles of the Innate Immune Response Using Multiplex Selected Reaction Monitoring–Mass Spectrometry
Zhao, Y., Tian, B., Edeh, C. B., & Brasier, A. R. (2013) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m112.023465 · Cited by 28
A distributable LC-MS/MS method for the measurement of serum thyroglobulin
Shi, J., Phipps, W. S., Owusu, B. Y., Henderson, C. M., Laha, T. J., Becker, J. O., Razavi, M., Emrick, M. A., & Hoofnagle, A. N. (2022) · Journal of Mass Spectrometry and Advances in the Clinical Lab · doi:10.1016/j.jmsacl.2022.09.005 · Cited by 28
Self-citation
Insights into the posttranslational structural heterogeneity of thyroglobulin and its role in the development, diagnosis, and management of benign and malignant thyroid diseases
Xavier, A. C. W., Maciel, R. M. B., Vieira, J. G. H., Dias-da-Silva, M. R., & Martins, J. R. M. (2016) · Archives of Endocrinology and Metabolism · doi:10.1590/2359-3997000000103 · Cited by 28
“Analysis of the polypeptide chain at the N-terminal end (1-2170) reveals an organized structure (Figure 1).”
Background
LC–MS/MS in Endocrinology: What is The Profit of The Last 5 Years?
Ackermans, M. T., & Endert, E. (2013) · Bioanalysis · doi:10.4155/bio.13.300 · Cited by 27
Acoustic ejection mass spectrometry empowers ultra-fast protein biomarker quantification
Puyvelde, B. V., Hunter, C. L., Zhgamadze, M., Savant, S. S., Wang, Y., Hoedt, E., Raedschelders, K., Pope, M., Huynh, C. A., Ramanujan, V. K., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-48563-z · Cited by 26
Mass spectrometry-based chemical mapping and profiling toward molecular understanding of diseases in precision medicine
Hu, Y., Wang, Z., Liu, L., Zhu, J., Zhang, D., Xu, M., Zhang, Y., Xu, F., & Chen, Y. (2021) · Chemical Science · doi:10.1039/d1sc00271f · Cited by 27
Mass Spectrometry in High-Throughput Clinical Biomarker Assays: Multiple Reaction Monitoring
Parker, C. E., Domański, D., Percy, A. J., Chambers, A. G., Camenzind, A. G., SMITH, D. S. H., & Borchers, C. H. (2012) · Topics in current chemistry · doi:10.1007/128_2012_353 · Cited by 26
Quantification of Alefacept, an immunosuppressive fusion protein in human plasma using a protein analogue internal standard, trypsin cleaved signature peptides and liquid chromatography tandem mass spectrometry
Halquist, M. S., & Karnes, H. T. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.02.034 · Cited by 26
Restructuring Proteomics Through Verification
Boja, E. S., Rivers, R. C., Kinsinger, C. R., Mesri, M., Hiltke, T., Rahbar, A. M., & Rodriguez, H. (2010) · Biomarkers in Medicine · doi:10.2217/bmm.10.92 · Cited by 26
Diffusing protein binders to intrinsically disordered proteins
Liu, C., Wu, K., Choi, H., Han, H., Zhang, X., Watson, J. L., Shijo, S., Bera, A. K., Kang, A., Brackenbrough, E., et al. (2024) · doi:10.1101/2024.07.16.603789 · Cited by 24
Self-citation
Proteomics beyond proteomics: toward clinical applications
Plymoth, A., & Hainaut, P. L. (2010) · Current Opinion in Oncology · doi:10.1097/cco.0b013e32834179c1 · Cited by 25
The Case for Laboratory Developed Procedures
Kaul, K. L., Sabatini, L. M., Tsongalis, G. J., Caliendo, A. M., Olsen, R. J., Ashwood, E. R., Bale, S. J., Benirschke, R. C., Carlow, D. C., Funke, B., et al. (2017) · Academic Pathology · doi:10.1177/2374289517708309 · Cited by 27
“Tg-IA methods had more false negatives and Tg-RIA had more false positives.(168) Although Tg-MS has not yet been incorporated into the current guidelines, the ATA guideline mentions it as a promising new technique.”
Method
Quantitative liquid chromatography tandem mass spectrometry analysis of macromolecules using signature peptides in biological fluids
Halquist, M. S., & Karnes, H. T. (2010) · Biomedical Chromatography · doi:10.1002/bmc.1545 · Cited by 25
Qualification and Verification of Protein Biomarker Candidates
Zhao, Y., & Brasier, A. R. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-41448-5_23 · Cited by 25
More sensitivity is always better: Measuring sub-clinical levels of serum thyroglobulin on a µLC–MS/MS system
Shuford, C. M., Johnson, J. S., Thompson, J. W., Holland, P. L., Hoofnagle, A. N., & Grant, R. P. (2020) · Clinical mass spectrometry · doi:10.1016/j.clinms.2020.01.001 · Cited by 24
Self-citation
Antibody based affinity capture LC-MS/MS in quantitative determination of proteins in biological matrices
Halvorsen, T. G., & Reubsaet, L. (2017) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2017.08.009 · Cited by 24
“(VIFDANAPVAVR) of thyroglobulin the challenge of auto-antibodies is circumvented as the capture step is placed, in time, after the digestion step [17, 48, 49].”
Background
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Kramer, G., Woolerton, Y., van Straalen, J. P., Vissers, J. P. C., Dekker, N., Langridge, J., Beynon, R. J., Speijer, D., Sturk, A., & Aerts, J. M. F. G. (2015) · PLoS ONE · doi:10.1371/journal.pone.0140097 · Cited by 26
CSF neurofilament light chain profiling and quantitation in neurological diseases
Leckey, C. A., Coulton, J. B., Giovannucci, T. A., He, Y., Aslanyan, A., Laban, R., Heslegrave, A., Doykov, I., Ammoscato, F., Chataway, J., et al. (2024) · Brain Communications · doi:10.1093/braincomms/fcae132 · Cited by 21
“There are few studies that directly compare protein and peptide antibody approaches for the same protein, but it has been suggested that peptide antibodies are more sensitive 43 and specific, 44 but with antibody efficiency being dependent on the structure of the protein or peptide in question.”
Discordance in thyroglobulin measurements by radioimmunoassay and immunometric assay: a useful means of identifying thyroglobulin assay interference
Crane, M. S., Strachan, M. W. J., Toft, A. D., & Beckett, G. J. (2013) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/0004563213480492 · Cited by 23
Liquid chromatography-tandem mass spectrometry in clinical laboratory protein measurement
Ma, Y., Wang, D., Li, H., Ma, X., Zou, Y., Mu, D., Yu, S., Cheng, X., & Qiu, L. (2024) · Clinica Chimica Acta · doi:10.1016/j.cca.2024.119846 · Cited by 22
Early post-treatment risk stratification of differentiated thyroid cancer: comparison of three high-sensitive Tg assays
Giovanella, L., Imperiali, M., Verburg, F. A., & Trimboli, P. (2017) · European Journal of Endocrinology · doi:10.1530/eje-17-0663 · Cited by 22
Mass spectrometry
Thomas, S. N. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-815499-1.00010-7 · Cited by 23
Quantification of Circulating D-dimer by Peptide Immunoaffinity Enrichment and Tandem Mass Spectrometry
Wang, W., Walker, N. D., Zhu, L.-J., Wu, W., Ge, L., Gutstein, D. E., Yates, N. A., Hendrickson, R. C., Ogletree, M. L., Cleary, M. A., et al. (2012) · Analytical Chemistry · doi:10.1021/ac301494d · Cited by 22
Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?
Arora, A., & Somasundaram, K. (2019) · BioEssays · doi:10.1002/bies.201800042 · Cited by 22
De novo design of high-affinity protein binders to bioactive helical peptides
Vázquez Torres, S., Leung, P. J. Y., Lutz, I. D., Venkatesh, P., Watson, J. L., Hink, F., Huynh, H.-H., Yeh, A. H.-W., Juergens, D., Bennett, N. R., et al. (2022) · doi:10.1101/2022.12.10.519862 · Cited by 22
Self-citation
Immunoaffinity techniques coupled to mass spectrometry for the analysis of human peptide hormones: advances and applications
Thomas, A., Schänzer, W., & Thevis, M. (2017) · Expert Review of Proteomics · doi:10.1080/14789450.2017.1362338 · Cited by 21
“also for large peptides and proteins [19,20,30,40].”
An Evaluation of an Aptamer for Use as an Affinity Reagent with MS: PCSK9 as an Example Protein
Gupta, V., Lassman, M. E., McAvoy, T., Lee, A. Y., Chappell, D. L., & Laterza, O. (2016) · Bioanalysis · doi:10.4155/bio-2016-0046 · Cited by 21
Strategies for improving sensitivity and selectivity for the quantitation of biotherapeutics in biological matrix using LC-MS/MS
Shen, J. X., Liu, G., & Zhao, Y. (2015) · Expert Review of Proteomics · doi:10.1586/14789450.2015.1024225 · Cited by 20
“Chaotropic agents such as urea and GnHCl have been traditionally used [14]; and other alternatives, such as heat denature [15] and the addition of surfactant [16] or organic solvents [17] have also been reported.”
Method
Differentiation and quantification of endogenous and recombinant-methionyl human leptin in clinical plasma samples by immunocapture/mass spectrometry
Wang, Y., & Heilig, J. S. (2012) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2012.06.018 · Cited by 19
Integration of proteomics in the molecular tumor board
Thiery, J., & Fahrner, M. (2023) · PROTEOMICS · doi:10.1002/pmic.202300002 · Cited by 21
“Measurementofserumthyroglobulin[96,97].”
Amino Acids, Peptides, and Proteins
Hortin, G. L. (2012) · Elsevier eBooks · doi:10.1016/b978-1-4160-6164-9.00021-4 · Cited by 18
Recent Progress in Selected Reaction Monitoring MS-Driven Plasma Protein Biomarker Analysis
Chiu, C., Randall, S. A., & Molloy, M. P. (2009) · Bioanalysis · doi:10.4155/bio.09.56 · Cited by 17
Laboratory services for thyroglobulin and implications for monitoring of differentiated thyroid cancer
CLARK, P. M. S. (2009) · Journal of Clinical Pathology · doi:10.1136/jcp.2008.058024 · Cited by 17
Serum thyroglobulin in the monitoring of differentiated thyroid cancer
Evans, C., Tennant, S., & Perros, P. (2016) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.1080/00365513.2016.1210339 · Cited by 17
Analytical and clinical performance of thyroglobulin autoantibody assays in thyroid cancer follow-up
Katrangi, W., Grebe, S. K. G., & Algeciras‐Schimnich, A. (2017) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2017-0034 · Cited by 17
Influence of Thyroglobulin Autoantibodies on Thyroglobulin Levels Measured by Different Methodologies: IMA, LC-MS/MS, and RIA
Petrovic, I., LoPresti, J. S., Fatemi, S., Gianoukakis, A. G., Burman, K. D., Gomes-Lima, C. J., Nguyen, C. T., & Spencer, C. A. (2024) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/clinem/dgae286 · Cited by 16
Protein Target Quantification Decision Tree
Kim, J. W., & You, J. (2013) · International Journal of Proteomics · doi:10.1155/2013/701247 · Cited by 16
Antibody-Coupled Magnetic Beads Can Be Reused in Immuno-MRM Assays To Reduce Cost and Extend Antibody Supply
Zhao, L., Whiteaker, J. R., Voytovich, U. J., Ivey, R. G., & Paulovich, A. G. (2015) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00290 · Cited by 16
Cancer Proteomics and the Elusive Diagnostic Biomarkers
Bradshaw, R. A., Hondermarck, H., & Rodriguez, H. (2019) · PROTEOMICS · doi:10.1002/pmic.201800445 · Cited by 16
Tutorial review for peptide assays: An ounce of pre-analytics is worth a pound of cure
Maurer, J., Grouzmann, E., & Eugster, P. J. (2023) · Journal of Chromatography B · doi:10.1016/j.jchromb.2023.123904 · Cited by 15
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Lei, Z., Whiteaker, J. R., Pope, M. E., Kuhn, E. W., Jackson, A., Anderson, N. L., Pearson, T. W., Carr, S. A., & Paulovich, A. G. (2011) · Journal of Visualized Experiments · doi:10.3791/2812-v · Cited by 15
Thyroglobulin Assay Interferences: Clinical Usefulness of Mass-Spectrometry Methods
Barbesino, G., Algeciras‐Schimnich, A., & Bornhorst, J. A. (2022) · Journal of the Endocrine Society · doi:10.1210/jendso/bvac169 · Cited by 14
“All TgMS assays were run with the Mayo Clinic Laboratories assay as previously described [9, 22].”
BackgroundMethod
Antibodies as means for selective mass spectrometry
Boström, T., Ottosson, J. E. A., & Hober, S. (2015) · Journal of Chromatography B · doi:10.1016/j.jchromb.2015.10.042 · Cited by 14
Applications of Low-Flow LC–SRM for The Analysis of Large Molecules in Pharmaceutical R&D
Lassman, M. E., & Fernández‐Metzler, C. (2014) · Bioanalysis · doi:10.4155/bio.14.141 · Cited by 14
The pros and cons of increased trypsin-to-protein ratio in targeted protein analysis
Egeland, S. V., Reubsaet, L., & Halvorsen, T. G. (2016) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2016.02.011 · Cited by 14
Multiplexed Immunoaffinity Enrichment of Peptides with Anti-peptide Antibodies and Quantification by Stable Isotope Dilution Multiple Reaction Monitoring Mass Spectrometry
Kuhn, E. W., & Carr, S. A. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3524-6_9 · Cited by 14
Serum Insulin-like Growth Factor I Quantitation by Mass Spectrometry: Insights for Protein Quantitation with this Technology.
Kam, R. K. T., Ho, C. S., & Chan, M. H. M. (2016) · PubMed Central · Cited by 14
High-Throughput, Sensitive LC-MS Quantification of Biotherapeutics and Biomarkers Using Antibody-Free, Peptide-Level, Multiple-Mechanism Enrichment via Strategic Regulation of pH and Ionic and Solvent Strengths
An, B., Zhang, M., Pu, J., Shen, S., Qu, Y., Chen, Y.-J., Huo, S., Wang, X., Polli, J. R., Balthasar, J. P., et al. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.8b05046 · Cited by 14
Well differentiated follicular thyroid neoplasia: Impact of molecular and technological advances on detection, monitoring and treatment
Gianoukakis, A. G., Giannelli, S. M., Salameh, W. A., & McPhaul, L. (2010) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2010.11.009 · Cited by 14
“Due to the poor reliability of Tg measurements by IMA or RIA, ecent attempts have been made to measure Tg by mass spectromtry (Hoofnagle et al., 2008; Clarke et al., 2010).”
Method
To elute or not to elute in immunocapture bottom-up LC–MS
Levernæs, M. C. S., Broughton, M. N., Reubsaet, L., & Halvorsen, T. G. (2017) · Journal of Chromatography B · doi:10.1016/j.jchromb.2017.03.044 · Cited by 14
Clinical laboratory verification of thyroglobulin concentrations in the presence of autoantibodies to thyroglobulin: comparison of EIA, radioimmunoassay and LC MS/MS measurements in an Urban Hospital
Wheeler, S., Liu, L., Blair, H. C., Sivak, R. A., Longo, N., Tischler, J., Mulvey, K., & Palmer, O. M. P. (2017) · BMC Research Notes · doi:10.1186/s13104-017-3050-6 · Cited by 14
Critical Considerations for Immunocapture Enrichment Lc–Ms Bioanalysis of Protein Therapeutics and Biomarkers
Zhao, Y., Gu, H., Zheng, N., & Zeng, J. (2018) · Bioanalysis · doi:10.4155/bio-2018-0062 · Cited by 14

Authors: Hoofnagle, A. N., Becker, J. O., Wener, M. H., & Heinecke, J. W.
Affiliations: Departments of Laboratory Medicine and
University of Washington (US)
Corresponding author: Hoofnagle, Andrew N
ORCID: Hoofnagle, Andrew N
Becker, Jessica O
Wener, Mark H
Heinecke, Jay W
Venue: Clinical Chemistry
Publisher: Oxford University Press (OUP)
Published: 2008-11-01
Volume: 54
Issue: 11
Pages: 1796-1804
ISSN: 0009-9147 (ISSN-L)
1530-8561
DOI: 10.1373/clinchem.2008.109652
PMC: PMC2739673
OpenAlex: W2167033601
Cited by: 314
References: 34
Citations by year: 2026: 2 · 2025: 9 · 2024: 12 · 2023: 13 · 2022: 9 · 2021: 10 · 2020: 16 · 2019: 9 · 2018: 16 · 2017: 21 · 2016: 25 · 2015: 15 · 2014: 28 · 2013: 26 · 2012: 31
FWCI: 10.09
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations: Clinical Chemistry (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: University of Washington
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
Keywords: thyroglobulin, tumor markers, immunoassay interference, polyclonal antibodies, tryptic peptides, multiple reaction monitoring, detection limit
MeSH terms: Amino Acid Sequence, Chromatography, Affinity, Chromatography, High Pressure Liquid, Humans, Immunoassay, Peptide Fragments, Peptide Mapping, Reference Standards, Sensitivity and Specificity, Thyroglobulin, Reproducibility of Results, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Quantification of Thyroglobulin, a Low-Abundance Serum Protein, by Immunoaffinity Peptide Enrichment and Tandem Mass Spectrometry

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks