CoChat
Sign Up
Cited by 160Clinical Laboratory Practices and Quality ControlDiabetes Management and ResearchBacterial Identification and Susceptibility Testing

The DOI matches its Crossref record; 8 of the 9 references it cites were found in a registry or an index.

Acidification of Blood Is Superior to Sodium Fluoride Alone as an Inhibitor of Glycolysis

Gambino, Raymond, Piscitelli, Janet, Ackattupathil, Tomy A, Theriault, Judy L, Andrin, Reynaldo D, Sanfilippo, Michael L, Etienne, Monina·2009·Clinical Chemistry
DOI 10.1373/clinchem.2008.121707
Open this paper in CoChat — free account

Abstract

Background: Sodium fluoride is the preferred agent to inhibit glycolysis. Its action is not immediate, however, and complete inhibition is delayed for up to 4 hours. A more effective method is needed. Acidification of blood combined with the addition of NaF and EDTA appears to be such a method. We studied whether acidification was indeed more effective than NaF. Methods: We conducted 6 independent studies over a 10-month period at 3 Quest Diagnostics laboratory sites. In each study, we drew venous blood from 6–24 nonfasting employee volunteers into 3 or 4 different serum- or plasma-collection tubes, which were stored under different conditions and aliquoted at different times. We analyzed the aliquots in duplicate by means of a hexokinase-based enzymatic method. Results: The mean glucose concentration decreased by 0.3% at 2 h and by 1.2% at 24 h when blood was drawn into tubes containing citrate buffer, NaF, and EDTA. In contrast, the mean glucose concentration decreased by 4.6% at 2 h and by 7.0% at 24 h when blood was drawn into tubes containing NaF and sodium oxalate. Conclusions: Acidification should replace NaF alone as the recommended method for obtaining an accurate glucose concentration. Diagnostic cut points based on blood samples collected into tubes containing NaF as the only inhibitor of glycolysis are likely to be too low.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1373/clinchem.2008.121707

Cites

8 of 8 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 9 references

Cited by

Sorted by citations

Items 101–160 of 160

Related

See all

Metadata

Authors
Gambino, R., Piscitelli, J., Ackattupathil, T. A., Theriault, J. L., Andrin, R. D., Sanfilippo, M. L., & Etienne, M.
Affiliations
  • Quest Diagnostics (United States) (US)
  • Teterboro, NJ
  • West Hills, CA, and
  • Miramar, FL
Corresponding author
Gambino, Raymond
Venue
Clinical Chemistry
Publisher
Oxford University Press (OUP)
Published
2009-05-01
Volume
55
Issue
5
Pages
1019-1021
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
DOI
10.1373/clinchem.2008.121707
OpenAlex
W2156782964
Cited by
160
References
9
Citations by year
2026: 2 · 2025: 5 · 2024: 8 · 2023: 4 · 2022: 5 · 2021: 9 · 2020: 10 · 2019: 12 · 2018: 13 · 2017: 10 · 2016: 14 · 2015: 11 · 2014: 9 · 2013: 12 · 2012: 16
FWCI
7.98
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Clinical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords
sodium fluoride, glycolysis inhibition, EDTA, sodium oxalate, blood collection tubes, serum glucose, plasma glucose, blood sample storage, diagnostic thresholds
MeSH terms
Blood, Blood Glucose, Blood Preservation, Citrates, Diabetes Mellitus, Glycolysis, Humans, Hydrogen-Ion Concentration, Sodium Fluoride, Specimen Handling

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review