Neutrophils Are the Predominant Infected Phagocytic Cells in the Airways of Patients With Active Pulmonary TB
Open accessAbstract
Background The exact role of neutrophils in the pathogenesis of TB is poorly understood. Recent evidence suggests that neutrophils are not simply scavenging phagocytes in Mycobacterium tuberculosis (Mtb) infection.Methods Three different types of clinical specimens from patients with active pulmonary TB who underwent lung surgery were examined: sputum, BAL fluid, and cavity contents. Differential cell separation and quantification were performed for intracellular and extracellular bacteria, and bacterial length was measured using microscopy.Results Neutrophils were more abundant than macrophages in sputum (86.6% +/- 2.2% vs 8.4% +/- 1.3%) and in BAL fluid (78.8% +/- 5.8% vs 11.8% +/- 4.1%). Inside the cavity, lymphocytes (41.3% +/- 11.2%) were the most abundant cell type, followed by neutrophils (38.8% +/- 9.4%) and macrophages (19.5% +/- 7.5%). More intracellular bacilli were found in neutrophils than macrophages in sputum (67.6% +/- 5.6% vs 25.2% +/- 6.5%), in BAL fluid (65.1% +/- 14.4% vs 28.3% +/- 11.6%), and in cavities (61.8% +/- 13.3% vs 23.9% +/- 9.3%). The lengths of Mtb were shortest in cavities (1.9+/- 0.1 microm), followed by in sputum (2.9 +/- 0.1 microm) and in BAL fluid (3.6 +/- 0.2 microm).Conclusions Our results show that neutrophils are the predominant cell types infected with Mtb in patients with TB and that these intracellular bacteria appear to replicate rapidly. These results are consistent with a role for neutrophils in providing a permissive site for a final burst of active replication of the bacilli prior to transmission.
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PMNs are important in containment of mycobacteria following their release from apoptotic/necrotic macrophages.
“PMNs are of importance in containment of mycobacteria following their release from apoptotic/necrotic macrophages (118, 119).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [118]
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- Authors
- Eum, S.-Y., Kong, J.-H., Hong, M.-S., Lee, Y.-J., Kim, J.-H., Hwang, S.-H., Cho, S.-N., Via, L. E., & Barry, C. E.
- Affiliations
- International Tuberculosis Research Center (KR)
- Masan National Tuberculosis Hospital (KR)
- Yonsei University (KR)
- National Institutes of Health (US)
- Corresponding author
- Eum, Seok-Yong
- Venue
- Chest
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 137
- Issue
- 1
- Pages
- 122-128
- ISSN
- 0012-3692 (ISSN-L)
- PMC
- PMC2803122
- OpenAlex
- W2081325862
- Cited by
- 548
- References
- 47
- Citations by year
- 2026: 24 · 2025: 34 · 2024: 30 · 2023: 37 · 2022: 37 · 2021: 35 · 2020: 33 · 2019: 42 · 2018: 46 · 2017: 33 · 2016: 30 · 2015: 42 · 2014: 46 · 2013: 24 · 2012: 29
- FWCI
- 12.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- CHEST Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- Funders
- National Institutes of Health (37882, Z01 AI000783-11)
- Wellcome Trust (37882, Z01 AI000783-11)
- Bill and Melinda Gates Foundation (37882, Z01 AI000783-11)
- National Institute of Allergy and Infectious Diseases (37882, Z01 AI000783-11)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- neutrophils, Mycobacterium tuberculosis, active pulmonary tuberculosis, intracellular bacteria, phagocytosis, macrophages, bronchoalveolar lavage fluid, sputum, pulmonary cavities, bacterial replication, tuberculosis pathogenesis
- MeSH terms
- Adult, Bronchoalveolar Lavage Fluid, Bronchoscopy, Cell Count, Follow-Up Studies, Humans, Middle Aged, Mycobacterium tuberculosis, Neutrophils, Phagocytes, Prognosis, Sputum, Tuberculosis, Pulmonary, Young Adult
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