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The Bradford Method for Protein Quantitation
Abstract
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford ( 1 ) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples ( see Note 1 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
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Metadata
- Authors
- Kruger, N. J.
- Venue
- Basic Protein and Peptide Protocols
- Publisher
- Humana Press
- Published
- 2003-11-14
- Volume
- 32
- Pages
- 9-16
- OpenAlex
- W2244276231
- Cited by
- 1,264
- References
- 1
- FWCI
- 23.24
- Type
- book chapter
- Language
- en
- Open access
- closed
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