Regulation of pituitary growth hormone-secretagogue and growth hormone-releasing hormone receptor RNA expression in young Dwarf rats
Open accessAbstract
Growth hormone-secretagogue receptor (GHSR) RNA is known to be expressed in the hypothalamus and pituitary. Since endogenous GH secretagogue (GHS) is still unknown, the physiological role of GHS and GHSR in growth is not well understood. In this study, we have determined the effects of growth hormone in GH-releasing hormone receptor (GHRHR) and GHSR RNA expression in spontaneous Dwarf rats (SDRs) which are deficient in GH secretion, with or without GH replacement. Twenty-five-day-old SDRs received daily s.c. injection of human GH (40 microg/kg BW x 2/day) or control solution for two weeks. On day 40, the rats were sacrificed by decapitation and the pituitaries were immediately removed and quickly frozen. Total RNA was extracted from the pituitary, and mRNA coding GHSR was detected and semi-quantitated by competitive RT-PCR. Pituitaries from control SDRs showed strong GHSR RNA expression and the expression level was 5 to 10 times higher in females than in males. When GH was replaced, GHSR RNA expression greatly decreased. Pituitary GHRHR RNA expression, determined by RNase Protection Assay, was similar in male and female control animals; and was also greatly reduced in rats treated with GH when compared to the control. These results suggest that the expression of both GHSR and GHRHR is regulated by growth hormone, presumably via changes in hypothalamic GHRH and/or endogenous GHS. The apparent sexual dimorphism in GHSR indicates different regulatory effects of sex steroid in young growing SDRs.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
E2 may upregulate receptor-dependent actions of GHRP.
“Laboratory data are consistent with a unifying notion that E 2 may upregulate receptor-dependent actions of GHRP.”
From Determinants of dual secretagogue drive of burst-like growth hormone secretion in premenopausal women studied under a selective estradiol clamp (2005) · cites this paper as [54]
The Abstract and RNA analysis section report substantially higher pituitary GHSR RNA in female than male dwarf rats and suggest sex-steroid regulation. They do not test E2 or show that it upregulates receptor-dependent responses to GHRP; the measured outcome was receptor RNA expression, not GHRP action. (LLM, conf=0.78)
Experimental — accuracy not yet measuredGene transcripts encoding the pituitary GHRP receptor are 2- to 10-fold more abundant in the adult female than male rodent pituitary.
“gene transcripts encoding the pituitary GHRP receptor are 2to 10-fold more abundant in the adult female than male rodent pituitary”
From Determinants of dual secretagogue drive of burst-like growth hormone secretion in premenopausal women studied under a selective estradiol clamp (2005) · cites this paper as [54]
The RNA analysis reports about tenfold higher pituitary GHSR RNA in female than male spontaneous dwarf rats; the study examined young rats at day 40, not adults. The Discussion also reports that GHSR expression became higher in males in normal rats at day 45. The source therefore does not support the claim’s generalization to adult rodents or its 2-to-10-fold range. (LLM, conf=0.88)
Experimental — accuracy not yet measuredE2 induces transcription of the human GHRP receptor gene in vitro.
“and E 2 induces transcription of the human GHRP receptor gene in vitro (23,54,55).”
From Determinants of dual secretagogue drive of burst-like growth hormone secretion in premenopausal women studied under a selective estradiol clamp (2005) · cites this paper as [54]
The Abstract and RNA analysis discuss GHSR RNA expression in dwarf-rat pituitaries, including differences between males and females and suppression after GH treatment. A search for E2 or estradiol-induced transcription of the human GHRP receptor gene in vitro found no passage addressing that claim; the rat findings do not establish or contradict it. Page numbers were not available in our copy. (LLM, conf=0.75)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A Receptor in Pituitary and Hypothalamus That Functions in Growth Hormone Release
Howard, A., Feighner, S., Cully, D., Arena, J. P., Liberator, P., Rosenblum, C., Hamelin, M., Hreniuk, D. L., Palyha, O., Anderson, J., et al. (1996) · Science · doi:10.1126/science.273.5277.974 · Cited by 2,106 · see citing works
Bowers, C. Y., Reynolds, G. A., DURHAM, D., Barrera, C. M., Pezzoli, S. S., Thorner, M. O., Forloni, F., Pinchera, A., & Faglia, G. (1990) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-70-4-975 · Cited by 316 · see citing works
Takeuchi, T., SUZUKI, H., Sakurai, S., et al. (1990) · Endocrinology · doi:10.1210/endo-126-1-31 · Cited by 101 · see citing works
Kamegai, J., Unterman, T. G., Frohman, L. A., & Kineman, R. D. (1998) · Endocrinology · doi:10.1210/endo.139.8.6136 · Cited by 70 · see citing works
Kamegai, J., Wakabayashi, I., Miyamoto, K., Unterman, T. G., Kineman, R. D., & Frohman, L. A. (1998) · Neuroendocrinology · doi:10.1159/000054379 · Cited by 51 · see citing works
Sakuma, S., Ishikawa, H., & OKUMA, S. (1990) · Brain Research · doi:10.1016/0006-8993(90)91268-l · Cited by 4 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Erickson, D., Keenan, D. M., Farhy, L., Mielke, K., Bowers, C. Y., & Veldhuis, J. D. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-1621 · Cited by 21 · see citing works
- Authors
- HORIKAWA, R., TACHIBANA, T., KATSUMATA, N., ISHIKAWA, H., & TANAKA, T.
- Affiliations
- Endocrine Research Laboratoy, National Children';s Medical Research Center
- Jikei University School of Medicine (JP)
- Venue
- Endocrine Journal
- Publisher
- Japan Endocrine Society
- Published
- 2000
- Volume
- 47
- Issue
- SupplMarch
- Pages
- S53-S56
- ISSN
- 0918-8959 (ISSN-L)
- 1348-4540
- OpenAlex
- W2134884098
- Semantic Scholar
- 9fe21436d30639efb2096eca1f0a7bea07f8f191
- Cited by
- 16
- References
- 6
- Citations by year
- 2025: 1 · 2018: 1 · 2012: 2
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Endocrine Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pituitary Gland Disorders and Treatments
- Keywords
- growth hormone secretagogue receptor, growth hormone-releasing hormone receptor, pituitary gland, dwarf rats, growth hormone replacement therapy, growth hormone regulation, sex differences, gene expression regulation
- MeSH terms
- Animals, Animals, Newborn, Dwarfism, Female, Humans, Injections, Subcutaneous, Male, Pituitary Gland, Rats, Mutant Strains, Receptors, Cell Surface, RNA, Messenger, Sex Characteristics, Receptors, Neuropeptide, Receptors, Pituitary Hormone-Regulating Hormone, Human Growth Hormone, Receptors, G-Protein-Coupled, Rats, Receptors, Ghrelin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
