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Glutathione Peroxidase, V. The kinetic mechanism
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“During the enzymatic reduction of hydrogen peroxide, Sec undergoes sequential oxidation by hydrogen peroxide to form a selenenic acid, Se-OH, followed by reaction with GSH to form a Se-SG intermediate before a second GSH combines to form GSSG, resolving the active site to Se-H (133, 278).”
From Redox regulation of mitochondrial function (2012) · cites this paper as [133]
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Chem in Biochem Gue - roult Enzymol / this Biochim
Boivin (1969) · BioL Clin BioL Chem Biochem J Biophys Acta
UnresolvedUsing the terminology of Clelandt this mecha - Klin Wochemchr Biochim / Lab Clin
Flohe (1971) · Biophys Acta
UnresolvedChim Brit
Necheles (1970) · Acta J Haematol
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[12] Assays of glutathione peroxidase
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“Enzymatic reduction of peroxides involves the formation of intermediate stable modifications to the Sec active site (127, 128, 204, 348, 369).”
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“Thus clearly one advantage of maintaining intracellular H O levels below 0.7 µ M is preventing the inadvertent oxidative inhibition of the numerous thiolate-dependent enzymes in the cell.”
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“Importantly, the Sec functionality in the mammalian GPxs is a redox-active site (Se-H), similar to Cys (S-H).”
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Metadata
- Authors
- Flohé, L., Loschen, G., Günzler, W. A., & Eichele, E.
- ORCID
- Flohé, Leopold
- Venue
- Hoppe-Seyler´s Zeitschrift für physiologische Chemie
- Publisher
- Walter de Gruyter GmbH
- Published
- 1972-01
- Volume
- 353
- Issue
- 1
- Pages
- 987-1000
- ISSN
- 0018-4888 (ISSN-L)
- OpenAlex
- W2002449018
- Cited by
- 340
- References
- 3
- Citations by year
- 2025: 4 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 5 · 2020: 11 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 4 · 2015: 10 · 2014: 13 · 2013: 11 · 2012: 6
- FWCI
- 4.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hoppe-Seyler´s Zeitschrift für physiologische Chemie (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Keywords
- glutathione peroxidase, enzyme kinetics, kinetic mechanism, hydrogen peroxide reduction, glutathione
- MeSH terms
- Animals, Cattle, Chemistry, Physical, Coumarins, Enzyme Activation, Glutathione, Hydrogen-Ion Concentration, Kinetics, Mathematics, Oxidation-Reduction, Peroxidases, Peroxides, Polarography, Spectrophotometry, Sulfates, Sulfhydryl Compounds, Titanium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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